IGVFDS2157LEAI

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS2157LEAI/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS2157LEAI",
    "aliases": [
        "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep1-BinD_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS2157LEAI](/measurement-sets/IGVFDS2157LEAI/) has sequence file(s) [IGVFFI0801ZZJK](/sequence-files/IGVFFI0801ZZJK/), [IGVFFI5025QRHM](/sequence-files/IGVFFI5025QRHM/), [IGVFFI1318ZQGJ](/sequence-files/IGVFFI1318ZQGJ/), [IGVFFI4963SYZF](/sequence-files/IGVFFI4963SYZF/), [IGVFFI0613CTPO](/sequence-files/IGVFFI0613CTPO/), [IGVFFI7627CVDO](/sequence-files/IGVFFI7627CVDO/), [IGVFFI9401SXDQ](/sequence-files/IGVFFI9401SXDQ/), [IGVFFI6750AEZS](/sequence-files/IGVFFI6750AEZS/), [IGVFFI3552NDJN](/sequence-files/IGVFFI3552NDJN/), [IGVFFI6347GLHK](/sequence-files/IGVFFI6347GLHK/), [IGVFFI5575ORYG](/sequence-files/IGVFFI5575ORYG/), [IGVFFI0934FSRE](/sequence-files/IGVFFI0934FSRE/), [IGVFFI3796VKYW](/sequence-files/IGVFFI3796VKYW/), [IGVFFI2885BUJM](/sequence-files/IGVFFI2885BUJM/), [IGVFFI5173QYPB](/sequence-files/IGVFFI5173QYPB/), [IGVFFI1029VVVW](/sequence-files/IGVFFI1029VVVW/), [IGVFFI1947JVYI](/sequence-files/IGVFFI1947JVYI/), [IGVFFI8953NBQX](/sequence-files/IGVFFI8953NBQX/), [IGVFFI9578QJAG](/sequence-files/IGVFFI9578QJAG/), [IGVFFI5232WRME](/sequence-files/IGVFFI5232WRME/), [IGVFFI0119WTXW](/sequence-files/IGVFFI0119WTXW/), [IGVFFI6126JLYO](/sequence-files/IGVFFI6126JLYO/), [IGVFFI2868VSMP](/sequence-files/IGVFFI2868VSMP/), [IGVFFI1497EGKW](/sequence-files/IGVFFI1497EGKW/), [IGVFFI0324IXMO](/sequence-files/IGVFFI0324IXMO/), [IGVFFI6770HRPL](/sequence-files/IGVFFI6770HRPL/), [IGVFFI5185VCMP](/sequence-files/IGVFFI5185VCMP/), [IGVFFI0983VSOC](/sequence-files/IGVFFI0983VSOC/), [IGVFFI0475TIQY](/sequence-files/IGVFFI0475TIQY/), [IGVFFI6825EIKJ](/sequence-files/IGVFFI6825EIKJ/), [IGVFFI3311TZLP](/sequence-files/IGVFFI3311TZLP/), [IGVFFI3741WODG](/sequence-files/IGVFFI3741WODG/), [IGVFFI6556RTWH](/sequence-files/IGVFFI6556RTWH/), [IGVFFI1565SEBA](/sequence-files/IGVFFI1565SEBA/), [IGVFFI2935CSSX](/sequence-files/IGVFFI2935CSSX/), [IGVFFI6284SCCX](/sequence-files/IGVFFI6284SCCX/), [IGVFFI0984OMZQ](/sequence-files/IGVFFI0984OMZQ/), [IGVFFI5041XJHH](/sequence-files/IGVFFI5041XJHH/), [IGVFFI2163HOMT](/sequence-files/IGVFFI2163HOMT/), [IGVFFI2431YNJV](/sequence-files/IGVFFI2431YNJV/), [IGVFFI2638KMFA](/sequence-files/IGVFFI2638KMFA/), [IGVFFI6615BFNS](/sequence-files/IGVFFI6615BFNS/), [IGVFFI7002BXQJ](/sequence-files/IGVFFI7002BXQJ/), [IGVFFI7104EGZQ](/sequence-files/IGVFFI7104EGZQ/), [IGVFFI6324HSQX](/sequence-files/IGVFFI6324HSQX/), [IGVFFI0233SWQQ](/sequence-files/IGVFFI0233SWQQ/), [IGVFFI0911GMRB](/sequence-files/IGVFFI0911GMRB/), [IGVFFI7698MFBM](/sequence-files/IGVFFI7698MFBM/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS2157LEAI/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS2157LEAI](/measurement-sets/IGVFDS2157LEAI/) has sequence file(s): [IGVFFI0801ZZJK](/sequence-files/IGVFFI0801ZZJK/), [IGVFFI5025QRHM](/sequence-files/IGVFFI5025QRHM/), [IGVFFI1318ZQGJ](/sequence-files/IGVFFI1318ZQGJ/), [IGVFFI4963SYZF](/sequence-files/IGVFFI4963SYZF/), [IGVFFI0613CTPO](/sequence-files/IGVFFI0613CTPO/), [IGVFFI7627CVDO](/sequence-files/IGVFFI7627CVDO/), [IGVFFI9401SXDQ](/sequence-files/IGVFFI9401SXDQ/), [IGVFFI6750AEZS](/sequence-files/IGVFFI6750AEZS/), [IGVFFI3552NDJN](/sequence-files/IGVFFI3552NDJN/), [IGVFFI6347GLHK](/sequence-files/IGVFFI6347GLHK/), [IGVFFI5575ORYG](/sequence-files/IGVFFI5575ORYG/), [IGVFFI0934FSRE](/sequence-files/IGVFFI0934FSRE/), [IGVFFI3796VKYW](/sequence-files/IGVFFI3796VKYW/), [IGVFFI2885BUJM](/sequence-files/IGVFFI2885BUJM/), [IGVFFI5173QYPB](/sequence-files/IGVFFI5173QYPB/), [IGVFFI1029VVVW](/sequence-files/IGVFFI1029VVVW/), [IGVFFI1947JVYI](/sequence-files/IGVFFI1947JVYI/), [IGVFFI8953NBQX](/sequence-files/IGVFFI8953NBQX/), [IGVFFI9578QJAG](/sequence-files/IGVFFI9578QJAG/), [IGVFFI5232WRME](/sequence-files/IGVFFI5232WRME/), [IGVFFI0119WTXW](/sequence-files/IGVFFI0119WTXW/), [IGVFFI6126JLYO](/sequence-files/IGVFFI6126JLYO/), [IGVFFI2868VSMP](/sequence-files/IGVFFI2868VSMP/), [IGVFFI1497EGKW](/sequence-files/IGVFFI1497EGKW/), [IGVFFI0324IXMO](/sequence-files/IGVFFI0324IXMO/), [IGVFFI6770HRPL](/sequence-files/IGVFFI6770HRPL/), [IGVFFI5185VCMP](/sequence-files/IGVFFI5185VCMP/), [IGVFFI0983VSOC](/sequence-files/IGVFFI0983VSOC/), [IGVFFI0475TIQY](/sequence-files/IGVFFI0475TIQY/), [IGVFFI6825EIKJ](/sequence-files/IGVFFI6825EIKJ/), [IGVFFI3311TZLP](/sequence-files/IGVFFI3311TZLP/), [IGVFFI3741WODG](/sequence-files/IGVFFI3741WODG/), [IGVFFI6556RTWH](/sequence-files/IGVFFI6556RTWH/), [IGVFFI1565SEBA](/sequence-files/IGVFFI1565SEBA/), [IGVFFI2935CSSX](/sequence-files/IGVFFI2935CSSX/), [IGVFFI6284SCCX](/sequence-files/IGVFFI6284SCCX/), [IGVFFI0984OMZQ](/sequence-files/IGVFFI0984OMZQ/), [IGVFFI5041XJHH](/sequence-files/IGVFFI5041XJHH/), [IGVFFI2163HOMT](/sequence-files/IGVFFI2163HOMT/), [IGVFFI2431YNJV](/sequence-files/IGVFFI2431YNJV/), [IGVFFI2638KMFA](/sequence-files/IGVFFI2638KMFA/), [IGVFFI6615BFNS](/sequence-files/IGVFFI6615BFNS/), [IGVFFI7002BXQJ](/sequence-files/IGVFFI7002BXQJ/), [IGVFFI7104EGZQ](/sequence-files/IGVFFI7104EGZQ/), [IGVFFI6324HSQX](/sequence-files/IGVFFI6324HSQX/), [IGVFFI0233SWQQ](/sequence-files/IGVFFI0233SWQQ/), [IGVFFI0911GMRB](/sequence-files/IGVFFI0911GMRB/), [IGVFFI7698MFBM](/sequence-files/IGVFFI7698MFBM/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS2157LEAI/"
            },
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM2364KNYW](/in-vitro-systems/IGVFSM2364KNYW/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM2364KNYW/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS2157LEAI](/measurement-sets/IGVFDS2157LEAI/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS2157LEAI/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
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            "@id": "/measurement-sets/IGVFDS4869FXYY/",
            "accession": "IGVFDS4869FXYY",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:48:10.868980+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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            "s3_uri": "s3://igvf-files/2023/12/13/8d7d0f68-9fcf-4fc2-b90b-bbb299dc409e/IGVFFI7698MFBM.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep2-FFRep1_BinD-PCRrep4_S232_I1_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS6299IJHG/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:09:03.423660+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM2364KNYW/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM2364KNYW",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep1-BinD"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "released",
            "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T10:00:53.638119+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "60ebb812-6529-42de-8be6-b9bbe8fd9474"
}