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IGVFDS2133JAFB

released
File Set Type
experimental data
Summary
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
  • GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane4), male, Homo sapiens modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library
Construct Library Sets
  • IGVFDS7581DNXF guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
Protocols
Aliases
jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane4_exprs
Publications

Sequencing Results (Illumina)

8 items
fastq
reads
R2
1
AAAMCTVHV
1
7.5 GB
Jay Shendure, UW
validated
fastq
reads
R1
1
AAAMCTVHV
1
3.3 GB
Jay Shendure, UW
validated
fastq
reads
R2
1
AAAMCTVHV
2
7.3 GB
Jay Shendure, UW
validated
fastq
reads
I2
1
AAAMCTVHV
2
843 MB
Jay Shendure, UW
validated
fastq
reads
I2
1
AAAMCTVHV
1
907 MB
Jay Shendure, UW
validated
fastq
reads
I1
1
AAAMCTVHV
1
922 MB
Jay Shendure, UW
validated
fastq
reads
I1
1
AAAMCTVHV
2
867 MB
Jay Shendure, UW
validated
fastq
reads
R1
1
AAAMCTVHV
2
3.2 GB
Jay Shendure, UW
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane4), male, Homo sapiens modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_WTC11
male
Homo sapiens

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
scCRISPR activation screen: differential gene expression quantifications, sparse gene count matrix
  • jay-shendure:analysis_set_CRISPRa_ipscNeurons
Jay Shendure, UW

Auxiliary Datasets

1 item
Accession
Type
Summary
Files
Aliases
Lab
AuxiliarySet
gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlecell_iPSC-derived-neurons_VPH_10Xlane4_CS1
Jay Shendure, UW

Attribution

Award
HG011966
Principal Investigator(s)
Contact P.I.
Jay Shendure
Lab
Jay Shendure, UW