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IGVFDS1817YVNX

released
File Set Type
experimental data
Summary
mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Protocols
Aliases
jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinB-PCR1_S62
jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinB-PCR2_S68
jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinB-PCR3_S158
jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinB-PCR4_S164

Sequencing Results

4 items
Read Names
fastq
reads
1
TGTAATCA:ATTG
20 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
CGCCTTCC:CTCT
16 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
CGCCTTCC:ATTG
26 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TGTAATCA:CTCT
12 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting accessible genome regions in many genomic loci

Auxiliary Datasets

1 item
Accession
Type
Summary
Aliases
Lab
AuxiliarySet
gRNA sequencing for mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinB-PCR1_S62
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinB-PCR2_S68
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinB-PCR3_S158
  • jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinB-PCR4_S164
Jesse Engreitz, Stanford

Documents

1 item
Page
Description
Type
Download
Preview
overview of experimental design
experimental protocol

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford