IGVFDS1769XASS

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS1769XASS/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS1769XASS",
    "aliases": [
        "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep4-BinC_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS1769XASS](/measurement-sets/IGVFDS1769XASS/) has sequence file(s) [IGVFFI0449QEQC](/sequence-files/IGVFFI0449QEQC/), [IGVFFI6063DALC](/sequence-files/IGVFFI6063DALC/), [IGVFFI0314COCE](/sequence-files/IGVFFI0314COCE/), [IGVFFI5138AXYH](/sequence-files/IGVFFI5138AXYH/), [IGVFFI2712KRCM](/sequence-files/IGVFFI2712KRCM/), [IGVFFI6124INLY](/sequence-files/IGVFFI6124INLY/), [IGVFFI1462ZGBE](/sequence-files/IGVFFI1462ZGBE/), [IGVFFI5365XJLZ](/sequence-files/IGVFFI5365XJLZ/), [IGVFFI4246EPZW](/sequence-files/IGVFFI4246EPZW/), [IGVFFI4861XSVP](/sequence-files/IGVFFI4861XSVP/), [IGVFFI6126TQLZ](/sequence-files/IGVFFI6126TQLZ/), [IGVFFI5649PBDW](/sequence-files/IGVFFI5649PBDW/), [IGVFFI4627ZLDD](/sequence-files/IGVFFI4627ZLDD/), [IGVFFI8143HBMT](/sequence-files/IGVFFI8143HBMT/), [IGVFFI6910FIXP](/sequence-files/IGVFFI6910FIXP/), [IGVFFI8140VKJV](/sequence-files/IGVFFI8140VKJV/), [IGVFFI7017SNEL](/sequence-files/IGVFFI7017SNEL/), [IGVFFI3452KFFK](/sequence-files/IGVFFI3452KFFK/), [IGVFFI8639PCWG](/sequence-files/IGVFFI8639PCWG/), [IGVFFI3652ZRQC](/sequence-files/IGVFFI3652ZRQC/), [IGVFFI6004FALS](/sequence-files/IGVFFI6004FALS/), [IGVFFI3323SYLP](/sequence-files/IGVFFI3323SYLP/), [IGVFFI2857NZTI](/sequence-files/IGVFFI2857NZTI/), [IGVFFI6928SLKD](/sequence-files/IGVFFI6928SLKD/), [IGVFFI9892HLLP](/sequence-files/IGVFFI9892HLLP/), [IGVFFI7986YMMD](/sequence-files/IGVFFI7986YMMD/), [IGVFFI6949EUDY](/sequence-files/IGVFFI6949EUDY/), [IGVFFI6353YGMX](/sequence-files/IGVFFI6353YGMX/), [IGVFFI7751APGF](/sequence-files/IGVFFI7751APGF/), [IGVFFI2518KVBV](/sequence-files/IGVFFI2518KVBV/), [IGVFFI4168VZKE](/sequence-files/IGVFFI4168VZKE/), [IGVFFI5931PPLE](/sequence-files/IGVFFI5931PPLE/), [IGVFFI5447NVNB](/sequence-files/IGVFFI5447NVNB/), [IGVFFI6299BGIJ](/sequence-files/IGVFFI6299BGIJ/), [IGVFFI8602GTIC](/sequence-files/IGVFFI8602GTIC/), [IGVFFI3873TNLY](/sequence-files/IGVFFI3873TNLY/), [IGVFFI0012ASYH](/sequence-files/IGVFFI0012ASYH/), [IGVFFI1740UBUG](/sequence-files/IGVFFI1740UBUG/), [IGVFFI3588NQYI](/sequence-files/IGVFFI3588NQYI/), [IGVFFI4159MCTJ](/sequence-files/IGVFFI4159MCTJ/), [IGVFFI9959BPYD](/sequence-files/IGVFFI9959BPYD/), [IGVFFI6508ZJZV](/sequence-files/IGVFFI6508ZJZV/), [IGVFFI8705TDCF](/sequence-files/IGVFFI8705TDCF/), [IGVFFI2982ALMP](/sequence-files/IGVFFI2982ALMP/), [IGVFFI1964GDMU](/sequence-files/IGVFFI1964GDMU/), [IGVFFI5311ZOMV](/sequence-files/IGVFFI5311ZOMV/), [IGVFFI9447BCCQ](/sequence-files/IGVFFI9447BCCQ/), [IGVFFI4719ZPHD](/sequence-files/IGVFFI4719ZPHD/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS1769XASS/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS1769XASS](/measurement-sets/IGVFDS1769XASS/) has sequence file(s): [IGVFFI0449QEQC](/sequence-files/IGVFFI0449QEQC/), [IGVFFI6063DALC](/sequence-files/IGVFFI6063DALC/), [IGVFFI0314COCE](/sequence-files/IGVFFI0314COCE/), [IGVFFI5138AXYH](/sequence-files/IGVFFI5138AXYH/), [IGVFFI2712KRCM](/sequence-files/IGVFFI2712KRCM/), [IGVFFI6124INLY](/sequence-files/IGVFFI6124INLY/), [IGVFFI1462ZGBE](/sequence-files/IGVFFI1462ZGBE/), [IGVFFI5365XJLZ](/sequence-files/IGVFFI5365XJLZ/), [IGVFFI4246EPZW](/sequence-files/IGVFFI4246EPZW/), [IGVFFI4861XSVP](/sequence-files/IGVFFI4861XSVP/), [IGVFFI6126TQLZ](/sequence-files/IGVFFI6126TQLZ/), [IGVFFI5649PBDW](/sequence-files/IGVFFI5649PBDW/), [IGVFFI4627ZLDD](/sequence-files/IGVFFI4627ZLDD/), [IGVFFI8143HBMT](/sequence-files/IGVFFI8143HBMT/), [IGVFFI6910FIXP](/sequence-files/IGVFFI6910FIXP/), [IGVFFI8140VKJV](/sequence-files/IGVFFI8140VKJV/), [IGVFFI7017SNEL](/sequence-files/IGVFFI7017SNEL/), [IGVFFI3452KFFK](/sequence-files/IGVFFI3452KFFK/), [IGVFFI8639PCWG](/sequence-files/IGVFFI8639PCWG/), [IGVFFI3652ZRQC](/sequence-files/IGVFFI3652ZRQC/), [IGVFFI6004FALS](/sequence-files/IGVFFI6004FALS/), [IGVFFI3323SYLP](/sequence-files/IGVFFI3323SYLP/), [IGVFFI2857NZTI](/sequence-files/IGVFFI2857NZTI/), [IGVFFI6928SLKD](/sequence-files/IGVFFI6928SLKD/), [IGVFFI9892HLLP](/sequence-files/IGVFFI9892HLLP/), [IGVFFI7986YMMD](/sequence-files/IGVFFI7986YMMD/), [IGVFFI6949EUDY](/sequence-files/IGVFFI6949EUDY/), [IGVFFI6353YGMX](/sequence-files/IGVFFI6353YGMX/), [IGVFFI7751APGF](/sequence-files/IGVFFI7751APGF/), [IGVFFI2518KVBV](/sequence-files/IGVFFI2518KVBV/), [IGVFFI4168VZKE](/sequence-files/IGVFFI4168VZKE/), [IGVFFI5931PPLE](/sequence-files/IGVFFI5931PPLE/), [IGVFFI5447NVNB](/sequence-files/IGVFFI5447NVNB/), [IGVFFI6299BGIJ](/sequence-files/IGVFFI6299BGIJ/), [IGVFFI8602GTIC](/sequence-files/IGVFFI8602GTIC/), [IGVFFI3873TNLY](/sequence-files/IGVFFI3873TNLY/), [IGVFFI0012ASYH](/sequence-files/IGVFFI0012ASYH/), [IGVFFI1740UBUG](/sequence-files/IGVFFI1740UBUG/), [IGVFFI3588NQYI](/sequence-files/IGVFFI3588NQYI/), [IGVFFI4159MCTJ](/sequence-files/IGVFFI4159MCTJ/), [IGVFFI9959BPYD](/sequence-files/IGVFFI9959BPYD/), [IGVFFI6508ZJZV](/sequence-files/IGVFFI6508ZJZV/), [IGVFFI8705TDCF](/sequence-files/IGVFFI8705TDCF/), [IGVFFI2982ALMP](/sequence-files/IGVFFI2982ALMP/), [IGVFFI1964GDMU](/sequence-files/IGVFFI1964GDMU/), [IGVFFI5311ZOMV](/sequence-files/IGVFFI5311ZOMV/), [IGVFFI9447BCCQ](/sequence-files/IGVFFI9447BCCQ/), [IGVFFI4719ZPHD](/sequence-files/IGVFFI4719ZPHD/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS1769XASS/"
            },
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM4017CUIN](/in-vitro-systems/IGVFSM4017CUIN/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM4017CUIN/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS1769XASS](/measurement-sets/IGVFDS1769XASS/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS1769XASS/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
    "control_file_sets": [
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            "@id": "/measurement-sets/IGVFDS4869FXYY/",
            "accession": "IGVFDS4869FXYY",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:48:15.493363+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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            "@id": "/sequence-files/IGVFFI0449QEQC/",
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            "s3_uri": "s3://igvf-files/2023/12/13/cf057181-d656-437b-b963-971ba6a93ab7/IGVFFI4719ZPHD.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep2-FFRep4_BinC-PCRrep9_S369_R2_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS6299IJHG/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:08:18.751949+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM4017CUIN/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM4017CUIN",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep4-BinC"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "released",
            "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T10:24:49.464491+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "767ce0ed-5543-428e-bcfd-b7b95b95afa7"
}