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INDEXED
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MeasurementSet
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IGVFDS1675WFHV
IGVFDS1675WFHV
released
Json view
File Set Type
experimental data
Summary
activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Construct Library Sets
IGVFDS0821YMMV
Guide (sgRNA) library targeting transcription start sites in 24 genes
Protocols
https://www.protocols.io/view/perturb-seq-characterizing-regulators-of-t-cell-fu-c5s9y6h6
https://www.protocols.io/view/crispra-tiling-screens-c5rfy53n
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispra-gex-rep2
Publications
Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens
Sequencing Results (Illumina)
8 items
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI1766LEES
fastq
reads
R2
2
IGVFFI1392XJCR
Illumina NovaSeq 6000
4
16 GB
Charles Gersbach, Duke
validated
IGVFFI2784HYOZ
fastq
reads
R2
2
IGVFFI1530GXXJ
Illumina NovaSeq 6000
2
16 GB
Charles Gersbach, Duke
validated
IGVFFI2833ZOSW
fastq
reads
R2
2
IGVFFI4753EPLN
Illumina NovaSeq 6000
3
16 GB
Charles Gersbach, Duke
validated
IGVFFI3561WXFX
fastq
reads
R1
2
IGVFFI4753EPLN
Illumina NovaSeq 6000
3
6.3 GB
Charles Gersbach, Duke
validated
IGVFFI4210ZDGH
fastq
reads
R2
2
IGVFFI9602EQCE
Illumina NovaSeq 6000
1
16 GB
Charles Gersbach, Duke
validated
IGVFFI4347FRPM
fastq
reads
R1
2
IGVFFI9602EQCE
Illumina NovaSeq 6000
1
6.3 GB
Charles Gersbach, Duke
validated
IGVFFI5263FBFP
fastq
reads
R1
2
IGVFFI1530GXXJ
Illumina NovaSeq 6000
2
6.3 GB
Charles Gersbach, Duke
validated
IGVFFI6604BYNW
fastq
reads
R1
2
IGVFFI1392XJCR
Illumina NovaSeq 6000
4
6.3 GB
Charles Gersbach, Duke
validated
Samples
Donors
File Sets Using This Measurement Set as an Input
Auxiliary Datasets
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke