IGVFDS1396XIPC

{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS1396XIPC/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS1396XIPC",
    "aliases": [
        "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep1-FFrep3-BinD_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS1396XIPC](/measurement-sets/IGVFDS1396XIPC/) has sequence file(s) [IGVFFI5958XAYH](/sequence-files/IGVFFI5958XAYH/), [IGVFFI7160KUNG](/sequence-files/IGVFFI7160KUNG/), [IGVFFI7303IABX](/sequence-files/IGVFFI7303IABX/), [IGVFFI8426ZHMW](/sequence-files/IGVFFI8426ZHMW/), [IGVFFI4049GRTH](/sequence-files/IGVFFI4049GRTH/), [IGVFFI3816GCXD](/sequence-files/IGVFFI3816GCXD/), [IGVFFI0197ZDDZ](/sequence-files/IGVFFI0197ZDDZ/), [IGVFFI4181IYRY](/sequence-files/IGVFFI4181IYRY/), [IGVFFI8493XOVH](/sequence-files/IGVFFI8493XOVH/), [IGVFFI7287TFKX](/sequence-files/IGVFFI7287TFKX/), [IGVFFI9281ZLNH](/sequence-files/IGVFFI9281ZLNH/), [IGVFFI8774MQWM](/sequence-files/IGVFFI8774MQWM/), [IGVFFI0276SFMS](/sequence-files/IGVFFI0276SFMS/), [IGVFFI1871WLJI](/sequence-files/IGVFFI1871WLJI/), [IGVFFI7196EBQD](/sequence-files/IGVFFI7196EBQD/), [IGVFFI0506YTCH](/sequence-files/IGVFFI0506YTCH/), [IGVFFI2038OPEA](/sequence-files/IGVFFI2038OPEA/), [IGVFFI8817ENHK](/sequence-files/IGVFFI8817ENHK/), [IGVFFI6857FDZJ](/sequence-files/IGVFFI6857FDZJ/), [IGVFFI5002RGSX](/sequence-files/IGVFFI5002RGSX/), [IGVFFI8187HKZX](/sequence-files/IGVFFI8187HKZX/), [IGVFFI5359EENI](/sequence-files/IGVFFI5359EENI/), [IGVFFI5623MHQH](/sequence-files/IGVFFI5623MHQH/), [IGVFFI6634CWVO](/sequence-files/IGVFFI6634CWVO/), [IGVFFI8480EMDE](/sequence-files/IGVFFI8480EMDE/), [IGVFFI1499NEDD](/sequence-files/IGVFFI1499NEDD/), [IGVFFI9129UAJH](/sequence-files/IGVFFI9129UAJH/), [IGVFFI1435KWRB](/sequence-files/IGVFFI1435KWRB/), [IGVFFI7348TUSQ](/sequence-files/IGVFFI7348TUSQ/), [IGVFFI8804QLRI](/sequence-files/IGVFFI8804QLRI/), [IGVFFI7702TBET](/sequence-files/IGVFFI7702TBET/), [IGVFFI3037MBXC](/sequence-files/IGVFFI3037MBXC/), [IGVFFI3033JQZS](/sequence-files/IGVFFI3033JQZS/), [IGVFFI8556NNND](/sequence-files/IGVFFI8556NNND/), [IGVFFI8572ZPBP](/sequence-files/IGVFFI8572ZPBP/), [IGVFFI5422MSQB](/sequence-files/IGVFFI5422MSQB/), [IGVFFI2041FHBO](/sequence-files/IGVFFI2041FHBO/), [IGVFFI3012UPRA](/sequence-files/IGVFFI3012UPRA/), [IGVFFI7808BINH](/sequence-files/IGVFFI7808BINH/), [IGVFFI9153FVUD](/sequence-files/IGVFFI9153FVUD/), [IGVFFI6195VDMM](/sequence-files/IGVFFI6195VDMM/), [IGVFFI9932CRAN](/sequence-files/IGVFFI9932CRAN/), [IGVFFI4120TGLK](/sequence-files/IGVFFI4120TGLK/), [IGVFFI6236KMOB](/sequence-files/IGVFFI6236KMOB/), [IGVFFI6909NJMF](/sequence-files/IGVFFI6909NJMF/), [IGVFFI8669FHFY](/sequence-files/IGVFFI8669FHFY/), [IGVFFI4731UJZD](/sequence-files/IGVFFI4731UJZD/), [IGVFFI7501XOGW](/sequence-files/IGVFFI7501XOGW/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS1396XIPC/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS1396XIPC](/measurement-sets/IGVFDS1396XIPC/) has sequence file(s): [IGVFFI5958XAYH](/sequence-files/IGVFFI5958XAYH/), [IGVFFI7160KUNG](/sequence-files/IGVFFI7160KUNG/), [IGVFFI7303IABX](/sequence-files/IGVFFI7303IABX/), [IGVFFI8426ZHMW](/sequence-files/IGVFFI8426ZHMW/), [IGVFFI4049GRTH](/sequence-files/IGVFFI4049GRTH/), [IGVFFI3816GCXD](/sequence-files/IGVFFI3816GCXD/), [IGVFFI0197ZDDZ](/sequence-files/IGVFFI0197ZDDZ/), [IGVFFI4181IYRY](/sequence-files/IGVFFI4181IYRY/), [IGVFFI8493XOVH](/sequence-files/IGVFFI8493XOVH/), [IGVFFI7287TFKX](/sequence-files/IGVFFI7287TFKX/), [IGVFFI9281ZLNH](/sequence-files/IGVFFI9281ZLNH/), [IGVFFI8774MQWM](/sequence-files/IGVFFI8774MQWM/), [IGVFFI0276SFMS](/sequence-files/IGVFFI0276SFMS/), [IGVFFI1871WLJI](/sequence-files/IGVFFI1871WLJI/), [IGVFFI7196EBQD](/sequence-files/IGVFFI7196EBQD/), [IGVFFI0506YTCH](/sequence-files/IGVFFI0506YTCH/), [IGVFFI2038OPEA](/sequence-files/IGVFFI2038OPEA/), [IGVFFI8817ENHK](/sequence-files/IGVFFI8817ENHK/), [IGVFFI6857FDZJ](/sequence-files/IGVFFI6857FDZJ/), [IGVFFI5002RGSX](/sequence-files/IGVFFI5002RGSX/), [IGVFFI8187HKZX](/sequence-files/IGVFFI8187HKZX/), [IGVFFI5359EENI](/sequence-files/IGVFFI5359EENI/), [IGVFFI5623MHQH](/sequence-files/IGVFFI5623MHQH/), [IGVFFI6634CWVO](/sequence-files/IGVFFI6634CWVO/), [IGVFFI8480EMDE](/sequence-files/IGVFFI8480EMDE/), [IGVFFI1499NEDD](/sequence-files/IGVFFI1499NEDD/), [IGVFFI9129UAJH](/sequence-files/IGVFFI9129UAJH/), [IGVFFI1435KWRB](/sequence-files/IGVFFI1435KWRB/), [IGVFFI7348TUSQ](/sequence-files/IGVFFI7348TUSQ/), [IGVFFI8804QLRI](/sequence-files/IGVFFI8804QLRI/), [IGVFFI7702TBET](/sequence-files/IGVFFI7702TBET/), [IGVFFI3037MBXC](/sequence-files/IGVFFI3037MBXC/), [IGVFFI3033JQZS](/sequence-files/IGVFFI3033JQZS/), [IGVFFI8556NNND](/sequence-files/IGVFFI8556NNND/), [IGVFFI8572ZPBP](/sequence-files/IGVFFI8572ZPBP/), [IGVFFI5422MSQB](/sequence-files/IGVFFI5422MSQB/), [IGVFFI2041FHBO](/sequence-files/IGVFFI2041FHBO/), [IGVFFI3012UPRA](/sequence-files/IGVFFI3012UPRA/), [IGVFFI7808BINH](/sequence-files/IGVFFI7808BINH/), [IGVFFI9153FVUD](/sequence-files/IGVFFI9153FVUD/), [IGVFFI6195VDMM](/sequence-files/IGVFFI6195VDMM/), [IGVFFI9932CRAN](/sequence-files/IGVFFI9932CRAN/), [IGVFFI4120TGLK](/sequence-files/IGVFFI4120TGLK/), [IGVFFI6236KMOB](/sequence-files/IGVFFI6236KMOB/), [IGVFFI6909NJMF](/sequence-files/IGVFFI6909NJMF/), [IGVFFI8669FHFY](/sequence-files/IGVFFI8669FHFY/), [IGVFFI4731UJZD](/sequence-files/IGVFFI4731UJZD/), [IGVFFI7501XOGW](/sequence-files/IGVFFI7501XOGW/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS1396XIPC/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS1396XIPC](/measurement-sets/IGVFDS1396XIPC/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS1396XIPC/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
    "control_file_sets": [
        {
            "@id": "/measurement-sets/IGVFDS4103BJLT/",
            "accession": "IGVFDS4103BJLT",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-THP1-PE2_Exp4_NegControl_AmpliconSequencing"
            ],
            "status": "preview"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2024-11-25T23:52:54.954181+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO7359LVPI/",
            "accession": "IGVFDO7359LVPI",
            "aliases": [
                "igvf:donor_of_THP1"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
    "files": [
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            "@id": "/sequence-files/IGVFFI5958XAYH/",
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            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2024-11-26T06:43:03.398662+00:00",
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                "status": "released",
                "term_name": "Illumina NovaSeq X"
            },
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            "upload_status": "validated"
        },
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            "@id": "/sequence-files/IGVFFI7160KUNG/",
            "accession": "IGVFFI7160KUNG",
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            "upload_status": "validated"
        },
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            "@id": "/sequence-files/IGVFFI7303IABX/",
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        },
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            "content_type": "reads",
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            "creation_timestamp": "2024-11-26T06:49:06.729770+00:00",
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            },
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        },
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            "@id": "/sequence-files/IGVFFI0197ZDDZ/",
            "accession": "IGVFFI0197ZDDZ",
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            ],
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            "creation_timestamp": "2024-11-26T06:50:16.970825+00:00",
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            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI4181IYRY/",
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            "content_type": "reads",
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            "upload_status": "validated"
        },
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            },
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            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI7287TFKX/",
            "accession": "IGVFFI7287TFKX",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep1-FFrep3-BinD-PCRrep4_R1"
            ],
            "content_type": "reads",
            "controlled_access": false,
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            "sequencing_platform": {
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                "term_name": "Illumina NovaSeq X"
            },
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            "submitted_file_name": "240426S024-Enhancer-THP1-PPIF_Enhancer_val_guides-Amp_PPIF_enhancer-BioRep1-FFrep3-BinD-PCRrep9_S555_I2_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI4731UJZD/",
            "accession": "IGVFFI4731UJZD",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep1-FFrep3-BinD-PCRrep2_I2"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2024-11-26T16:46:39.900007+00:00",
            "file_format": "fastq",
            "file_size": 6989540,
            "href": "/sequence-files/IGVFFI4731UJZD/@@download/IGVFFI4731UJZD.fastq.gz",
            "s3_uri": "s3://igvf-files/2024/11/26/cf92e0cd-ecc7-491b-a531-c1fa47db28fe/IGVFFI4731UJZD.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0022840/",
                "status": "released",
                "term_name": "Illumina NovaSeq X"
            },
            "status": "preview",
            "submitted_file_name": "240426S024-Enhancer-THP1-PPIF_Enhancer_val_guides-Amp_PPIF_enhancer-BioRep1-FFrep3-BinD-PCRrep2_S548_I2_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI7501XOGW/",
            "accession": "IGVFFI7501XOGW",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep1-FFrep3-BinD-PCRrep11_I2"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2024-11-26T17:08:19.588446+00:00",
            "file_format": "fastq",
            "file_size": 7192406,
            "href": "/sequence-files/IGVFFI7501XOGW/@@download/IGVFFI7501XOGW.fastq.gz",
            "s3_uri": "s3://igvf-files/2024/11/26/b540a6ac-5ace-4799-9e7b-3be61f9fc5c4/IGVFFI7501XOGW.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0022840/",
                "status": "released",
                "term_name": "Illumina NovaSeq X"
            },
            "status": "preview",
            "submitted_file_name": "240426S024-Enhancer-THP1-PPIF_Enhancer_val_guides-Amp_PPIF_enhancer-BioRep1-FFrep3-BinD-PCRrep11_S557_I2_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS4833ZRTZ/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM4077CGNC/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM4077CGNC",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep1-FFrep3-BinD"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS4955GEYO/",
                    "accession": "IGVFDS4955GEYO",
                    "file_set_type": "guide library",
                    "status": "preview",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
                    "status": "released",
                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "preview",
            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "preview",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2024-11-26T06:41:29.437505+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "86e7a45e-13f0-4cba-b924-949c1865d053"
}