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IGVFDS1334EHAI

released
File Set Type
experimental data
Summary
mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Construct Library Sets
  • IGVFDS5413RKIA guide (sgRNA) library targeting accessible genome regions in many genomic loci
Protocols
Aliases
jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinD-PCR1-Rep1_S28
jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinD-PCR2-Rep1_S34
jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinD-PCR3-Rep1_S82
jesse-engreitz:measurement_CRUDO_SSFA2-Auxin6hrs-FF1-BinD-PCR4-Rep1_S88

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Auxiliary Datasets

2 items
Files
AuxiliarySet
gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinD-PCR1-Rep1_S28
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinD-PCR2-Rep1_S34
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinD-PCR3-Rep1_S82
  • jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin6hrs-FF1-BinD-PCR4-Rep1_S88
Jesse Engreitz, Stanford
AuxiliarySet
cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_SSFA2-Auxin6hrs-FF1
Jesse Engreitz, Stanford

Documents

1 item
Page
Description
Type
Download
Preview
overview of experimental design
experimental protocol

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford