IGVFDS1330GUQX

{
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        "@id": "/assay-terms/OBI_0003660/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using single-cell RNA-seq"
    },
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        "INTERNAL_ACTION": [
            {
                "category": "NTR term ID",
                "detail": "Sample term for [NTR_0000753](/sample-terms/NTR_0000753/) has been newly requested. Ontology terms with a newly requested term ID are expected to have it updated following its addition to the appropriate ontology database.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_ntr_term_id",
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            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS1330GUQX](/measurement-sets/IGVFDS1330GUQX/) has sequence file(s): [IGVFFI7172LWWA](/sequence-files/IGVFFI7172LWWA/), [IGVFFI6581WIWZ](/sequence-files/IGVFFI6581WIWZ/), [IGVFFI6490BHDI](/sequence-files/IGVFFI6490BHDI/), [IGVFFI4138XCVE](/sequence-files/IGVFFI4138XCVE/), [IGVFFI0917LUUZ](/sequence-files/IGVFFI0917LUUZ/), [IGVFFI5283HPSZ](/sequence-files/IGVFFI5283HPSZ/), [IGVFFI5098UBYC](/sequence-files/IGVFFI5098UBYC/), [IGVFFI0679LZWL](/sequence-files/IGVFFI0679LZWL/), [IGVFFI6069FNXL](/sequence-files/IGVFFI6069FNXL/), [IGVFFI7971UKDC](/sequence-files/IGVFFI7971UKDC/), [IGVFFI7253CFAI](/sequence-files/IGVFFI7253CFAI/), [IGVFFI5111RYBQ](/sequence-files/IGVFFI5111RYBQ/), [IGVFFI2236RSMA](/sequence-files/IGVFFI2236RSMA/), [IGVFFI0457VHVR](/sequence-files/IGVFFI0457VHVR/), [IGVFFI1277WLJZ](/sequence-files/IGVFFI1277WLJZ/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS1330GUQX/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Auxiliary set [IGVFDS4288VPSU](/auxiliary-sets/IGVFDS4288VPSU/) has sequence file(s): [IGVFFI0292YEIE](/sequence-files/IGVFFI0292YEIE/), [IGVFFI9133PDUV](/sequence-files/IGVFFI9133PDUV/), [IGVFFI9889IEMK](/sequence-files/IGVFFI9889IEMK/), [IGVFFI6242AIOG](/sequence-files/IGVFFI6242AIOG/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
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            "title": "Jesse Engreitz"
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    "creation_timestamp": "2024-01-18T03:10:03.437656+00:00",
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            "submitted_file_name": "/oak/stanford/groups/engreitz/Users/kangh/TeloHAEC_Perturb-seq_2kG/210616_Broad_fastq/scRNAseq_2kG_2PMDox1_3_d_ATCTTTAG_L001_R2_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI1277WLJZ/",
            "accession": "IGVFFI1277WLJZ",
            "aliases": [
                "jesse-engreitz:sequence_file-teloHAEC_2kG_library-NovaSeq1-scRNAseq_2kG_2PMDox1_3_S1_L001_I1_001.fastq.gz"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2023-12-20T23:06:49.430834+00:00",
            "file_format": "fastq",
            "file_size": 2684269934,
            "href": "/sequence-files/IGVFFI1277WLJZ/@@download/IGVFFI1277WLJZ.fastq.gz",
            "s3_uri": "s3://igvf-files/2023/12/20/02754ea3-e367-40de-a311-21fcf5a739b9/IGVFFI1277WLJZ.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008637/",
                "status": "released",
                "term_name": "Illumina NovaSeq 6000"
            },
            "status": "preview",
            "submitted_file_name": "/oak/stanford/groups/engreitz/Users/kangh/TeloHAEC_Perturb-seq_2kG/210528_Broad_fastq/scRNAseq_2kG_2PMDox1_3_S1_L001_I1_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS7943IIWZ/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 86015c12-b9a6-43ba-9601-5d75aab4d39c was removed from this measurement set.",
    "preferred_assay_title": "Perturb-seq",
    "protocols": [
        "https://www.protocols.io/view/systematic-perturb-seq-to-discover-endothelial-cel-261ged73yv47/v1"
    ],
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM9151FQCE/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM9151FQCE",
            "aliases": [
                "jesse-engreitz:in_vitro_systems-scRNAseq_2kG_2PMDox1_3"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS1298TUYG/",
                    "accession": "IGVFDS1298TUYG",
                    "file_set_type": "guide library",
                    "status": "preview",
                    "summary": "guide (sgRNA) library targeting genes in many genomic loci"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
                    "modality": "interference",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/NTR_0000753/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:TeloHAEC"
                    ],
                    "status": "released",
                    "summary": "TeloHAEC",
                    "term_name": "TeloHAEC"
                }
            ],
            "status": "preview",
            "summary": "TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "preview",
    "submitted_by": {
        "@id": "/users/05fa0dc9-0e33-427c-9d1f-82393edc4a03/",
        "title": "Helen Kang"
    },
    "submitted_files_timestamp": "2023-12-20T23:00:22.655644+00:00",
    "summary": "CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci",
    "uuid": "478b1a64-b096-4085-9489-9f6cfb6d98d5"
}