IGVFDS1319XVLL

{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS1319XVLL/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS1319XVLL",
    "aliases": [
        "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep1-FFrep2-BinA_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS1319XVLL](/measurement-sets/IGVFDS1319XVLL/) has sequence file(s) [IGVFFI6157RLIA](/sequence-files/IGVFFI6157RLIA/), [IGVFFI0416YRLS](/sequence-files/IGVFFI0416YRLS/), [IGVFFI1886CXGZ](/sequence-files/IGVFFI1886CXGZ/), [IGVFFI9419YLFW](/sequence-files/IGVFFI9419YLFW/), [IGVFFI1999EXLH](/sequence-files/IGVFFI1999EXLH/), [IGVFFI0425TAES](/sequence-files/IGVFFI0425TAES/), [IGVFFI7039BUWJ](/sequence-files/IGVFFI7039BUWJ/), [IGVFFI0950CPRT](/sequence-files/IGVFFI0950CPRT/), [IGVFFI2628GCOT](/sequence-files/IGVFFI2628GCOT/), [IGVFFI1546NHZH](/sequence-files/IGVFFI1546NHZH/), [IGVFFI7710LIFV](/sequence-files/IGVFFI7710LIFV/), [IGVFFI9674BACC](/sequence-files/IGVFFI9674BACC/), [IGVFFI6508GSWD](/sequence-files/IGVFFI6508GSWD/), [IGVFFI6180DGKA](/sequence-files/IGVFFI6180DGKA/), [IGVFFI9206AUFI](/sequence-files/IGVFFI9206AUFI/), [IGVFFI4044PBRP](/sequence-files/IGVFFI4044PBRP/), [IGVFFI7554YPPS](/sequence-files/IGVFFI7554YPPS/), [IGVFFI8777MXXY](/sequence-files/IGVFFI8777MXXY/), [IGVFFI9241FNZY](/sequence-files/IGVFFI9241FNZY/), [IGVFFI3503BPKN](/sequence-files/IGVFFI3503BPKN/), [IGVFFI0019XPES](/sequence-files/IGVFFI0019XPES/), [IGVFFI0576ECSI](/sequence-files/IGVFFI0576ECSI/), [IGVFFI8033WMRT](/sequence-files/IGVFFI8033WMRT/), [IGVFFI5873SDKS](/sequence-files/IGVFFI5873SDKS/), [IGVFFI9724EAAQ](/sequence-files/IGVFFI9724EAAQ/), [IGVFFI9492AZKJ](/sequence-files/IGVFFI9492AZKJ/), [IGVFFI2564NSJV](/sequence-files/IGVFFI2564NSJV/), [IGVFFI8009HURM](/sequence-files/IGVFFI8009HURM/), [IGVFFI6909ADER](/sequence-files/IGVFFI6909ADER/), [IGVFFI4092GCVZ](/sequence-files/IGVFFI4092GCVZ/), [IGVFFI5700GLSH](/sequence-files/IGVFFI5700GLSH/), [IGVFFI9956YVYV](/sequence-files/IGVFFI9956YVYV/), [IGVFFI3712MJHT](/sequence-files/IGVFFI3712MJHT/), [IGVFFI6519RCQL](/sequence-files/IGVFFI6519RCQL/), [IGVFFI2843CVQX](/sequence-files/IGVFFI2843CVQX/), [IGVFFI5396LVCV](/sequence-files/IGVFFI5396LVCV/), [IGVFFI3451KSGC](/sequence-files/IGVFFI3451KSGC/), [IGVFFI2873DIIR](/sequence-files/IGVFFI2873DIIR/), [IGVFFI9949DCGR](/sequence-files/IGVFFI9949DCGR/), [IGVFFI0464IKFP](/sequence-files/IGVFFI0464IKFP/), [IGVFFI3201AANW](/sequence-files/IGVFFI3201AANW/), [IGVFFI3189ONQJ](/sequence-files/IGVFFI3189ONQJ/), [IGVFFI7767YDDR](/sequence-files/IGVFFI7767YDDR/), [IGVFFI3962IEJM](/sequence-files/IGVFFI3962IEJM/), [IGVFFI6761LLIA](/sequence-files/IGVFFI6761LLIA/), [IGVFFI3435YJPI](/sequence-files/IGVFFI3435YJPI/), [IGVFFI9435OMZV](/sequence-files/IGVFFI9435OMZV/), [IGVFFI6700IZYS](/sequence-files/IGVFFI6700IZYS/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS1319XVLL/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS1319XVLL](/measurement-sets/IGVFDS1319XVLL/) has sequence file(s): [IGVFFI6157RLIA](/sequence-files/IGVFFI6157RLIA/), [IGVFFI0416YRLS](/sequence-files/IGVFFI0416YRLS/), [IGVFFI1886CXGZ](/sequence-files/IGVFFI1886CXGZ/), [IGVFFI9419YLFW](/sequence-files/IGVFFI9419YLFW/), [IGVFFI1999EXLH](/sequence-files/IGVFFI1999EXLH/), [IGVFFI0425TAES](/sequence-files/IGVFFI0425TAES/), [IGVFFI7039BUWJ](/sequence-files/IGVFFI7039BUWJ/), [IGVFFI0950CPRT](/sequence-files/IGVFFI0950CPRT/), [IGVFFI2628GCOT](/sequence-files/IGVFFI2628GCOT/), [IGVFFI1546NHZH](/sequence-files/IGVFFI1546NHZH/), [IGVFFI7710LIFV](/sequence-files/IGVFFI7710LIFV/), [IGVFFI9674BACC](/sequence-files/IGVFFI9674BACC/), [IGVFFI6508GSWD](/sequence-files/IGVFFI6508GSWD/), [IGVFFI6180DGKA](/sequence-files/IGVFFI6180DGKA/), [IGVFFI9206AUFI](/sequence-files/IGVFFI9206AUFI/), [IGVFFI4044PBRP](/sequence-files/IGVFFI4044PBRP/), [IGVFFI7554YPPS](/sequence-files/IGVFFI7554YPPS/), [IGVFFI8777MXXY](/sequence-files/IGVFFI8777MXXY/), [IGVFFI9241FNZY](/sequence-files/IGVFFI9241FNZY/), [IGVFFI3503BPKN](/sequence-files/IGVFFI3503BPKN/), [IGVFFI0019XPES](/sequence-files/IGVFFI0019XPES/), [IGVFFI0576ECSI](/sequence-files/IGVFFI0576ECSI/), [IGVFFI8033WMRT](/sequence-files/IGVFFI8033WMRT/), [IGVFFI5873SDKS](/sequence-files/IGVFFI5873SDKS/), [IGVFFI9724EAAQ](/sequence-files/IGVFFI9724EAAQ/), [IGVFFI9492AZKJ](/sequence-files/IGVFFI9492AZKJ/), [IGVFFI2564NSJV](/sequence-files/IGVFFI2564NSJV/), [IGVFFI8009HURM](/sequence-files/IGVFFI8009HURM/), [IGVFFI6909ADER](/sequence-files/IGVFFI6909ADER/), [IGVFFI4092GCVZ](/sequence-files/IGVFFI4092GCVZ/), [IGVFFI5700GLSH](/sequence-files/IGVFFI5700GLSH/), [IGVFFI9956YVYV](/sequence-files/IGVFFI9956YVYV/), [IGVFFI3712MJHT](/sequence-files/IGVFFI3712MJHT/), [IGVFFI6519RCQL](/sequence-files/IGVFFI6519RCQL/), [IGVFFI2843CVQX](/sequence-files/IGVFFI2843CVQX/), [IGVFFI5396LVCV](/sequence-files/IGVFFI5396LVCV/), [IGVFFI3451KSGC](/sequence-files/IGVFFI3451KSGC/), [IGVFFI2873DIIR](/sequence-files/IGVFFI2873DIIR/), [IGVFFI9949DCGR](/sequence-files/IGVFFI9949DCGR/), [IGVFFI0464IKFP](/sequence-files/IGVFFI0464IKFP/), [IGVFFI3201AANW](/sequence-files/IGVFFI3201AANW/), [IGVFFI3189ONQJ](/sequence-files/IGVFFI3189ONQJ/), [IGVFFI7767YDDR](/sequence-files/IGVFFI7767YDDR/), [IGVFFI3962IEJM](/sequence-files/IGVFFI3962IEJM/), [IGVFFI6761LLIA](/sequence-files/IGVFFI6761LLIA/), [IGVFFI3435YJPI](/sequence-files/IGVFFI3435YJPI/), [IGVFFI9435OMZV](/sequence-files/IGVFFI9435OMZV/), [IGVFFI6700IZYS](/sequence-files/IGVFFI6700IZYS/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS1319XVLL/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS1319XVLL](/measurement-sets/IGVFDS1319XVLL/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS1319XVLL/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
    "control_file_sets": [
        {
            "@id": "/measurement-sets/IGVFDS4103BJLT/",
            "accession": "IGVFDS4103BJLT",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-THP1-PE2_Exp4_NegControl_AmpliconSequencing"
            ],
            "status": "preview"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2024-11-25T23:52:26.064614+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO7359LVPI/",
            "accession": "IGVFDO7359LVPI",
            "aliases": [
                "igvf:donor_of_THP1"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2024-11-26T06:32:48.321840+00:00",
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                "term_name": "Illumina NovaSeq X"
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            "upload_status": "validated"
        },
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            "@id": "/sequence-files/IGVFFI0416YRLS/",
            "accession": "IGVFFI0416YRLS",
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        },
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            "@id": "/sequence-files/IGVFFI0425TAES/",
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            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI1546NHZH/",
            "accession": "IGVFFI1546NHZH",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep1-FFrep2-BinA-PCRrep1_R1"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2024-11-26T06:25:15.129639+00:00",
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            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI7710LIFV/",
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            "href": "/sequence-files/IGVFFI9435OMZV/@@download/IGVFFI9435OMZV.fastq.gz",
            "s3_uri": "s3://igvf-files/2024/11/26/f78b6a7e-52c5-41dc-9a95-6f75cbc0f1d8/IGVFFI9435OMZV.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0022840/",
                "status": "released",
                "term_name": "Illumina NovaSeq X"
            },
            "status": "preview",
            "submitted_file_name": "240426S024-Enhancer-THP1-PPIF_Enhancer_val_guides-Amp_PPIF_enhancer-BioRep1-FFrep2-BinA-PCRrep11_S473_I2_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI6700IZYS/",
            "accession": "IGVFFI6700IZYS",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep1-FFrep2-BinA-PCRrep3_I2"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2024-11-26T16:18:04.243493+00:00",
            "file_format": "fastq",
            "file_size": 5990704,
            "href": "/sequence-files/IGVFFI6700IZYS/@@download/IGVFFI6700IZYS.fastq.gz",
            "s3_uri": "s3://igvf-files/2024/11/26/4fe035ca-cf77-415e-8591-955921bfabd0/IGVFFI6700IZYS.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0022840/",
                "status": "released",
                "term_name": "Illumina NovaSeq X"
            },
            "status": "preview",
            "submitted_file_name": "240426S024-Enhancer-THP1-PPIF_Enhancer_val_guides-Amp_PPIF_enhancer-BioRep1-FFrep2-BinA-PCRrep3_S465_I2_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS4833ZRTZ/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM7478MTAF/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM7478MTAF",
            "aliases": [
                "jesse-engreitz:240426S024-PPIFenhancervalidation-THP1_Amp_PPIF_enhancer-BioRep1-FFrep2-BinA"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS4955GEYO/",
                    "accession": "IGVFDS4955GEYO",
                    "file_set_type": "guide library",
                    "status": "preview",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0001253/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:THP-1"
                    ],
                    "status": "released",
                    "summary": "THP-1",
                    "term_name": "THP-1"
                }
            ],
            "status": "preview",
            "summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "preview",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2024-11-26T06:25:15.129639+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "570f7eb3-724c-4cae-9ded-d4932102dbf2"
}