IGVFDS1310KDLX

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

MYC

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS7857SYCK guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR1_Rep1_S3

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR1_Rep2_S58

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR2_Rep1_S9

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR2_Rep2_S64

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR3_Rep1_S15

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR3_Rep2_S70

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR4_Rep1_S21

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR4_Rep2_S76

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4577TQPO

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS4252ROMJ

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI2043YWNR, IGVFFI4506MWVS, IGVFFI1890PKGY, IGVFFI7334BOPO, IGVFFI5042RRSN, IGVFFI9404PGPV, IGVFFI8568LLGQ, IGVFFI0242VPKF

jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR1_Rep1_S3
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR1_Rep2_S58
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR2_Rep1_S9
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR2_Rep2_S64
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR3_Rep1_S15
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR3_Rep2_S70
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR4_Rep1_S21
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR4_Rep2_S76

Jesse Engreitz, Stanford

IGVFDS5951VSHM

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI2212HXUF