IGVFDS1310KDLX

released
File Set Type
experimental data
Summary
mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci
Assay Term
Targeted Genes
Sample Summaries
  • HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Protocols
Aliases
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR1_Rep1_S3
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR1_Rep2_S58
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR2_Rep1_S9
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR2_Rep2_S64
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR3_Rep1_S15
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR3_Rep2_S70
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR4_Rep1_S21
jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinC-PCR4_Rep2_S76

Sequencing Results

8 items
fastq
reads
1
TGTCGGAT:TCTC
32 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TCGGAATG:TCTC
14 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GCACATCT:TGCG
29 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
ACCAACTG:TGCG
32 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TCGGAATG:TGCG
39 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
ACCAACTG:TCTC
20 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
TGTCGGAT:TGCG
51 MB
Jesse Engreitz, Stanford
validated
fastq
reads
1
GCACATCT:TCTC
10.0 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_HCT116
male
Homo sapiens

Auxiliary Datasets

1 item
Accession
Type
Summary
Files
Aliases
Lab
AuxiliarySet
cell sorting for mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen sorted on the expression of MYC integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets
  • jesse-engreitz:auxiliary_sortparams_CRUDO_MYC-Auxin0hrs-FF1
Jesse Engreitz, Stanford

Documents

1 item
Page
Description
Type
Download
Preview
overview of experimental design
experimental protocol

Attribution