IGVFDS1298BFAZ

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

MYC

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS7857SYCK guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR1_Rep1_S1

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR1_Rep2_S56

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR2_Rep1_S7

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR2_Rep2_S62

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR3_Rep1_S13

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR3_Rep2_S68

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR4_Rep1_S19

jesse-engreitz:measurement_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR4_Rep2_S74

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3086DQMC

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS2805FQAD

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI8310ZXDQ, IGVFFI9473RAYZ, IGVFFI7962MDES, IGVFFI1423QMWB, IGVFFI7230YSEB, IGVFFI5243KTWV, IGVFFI8079GYLE, IGVFFI5803HMJL

jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR1_Rep1_S1
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR1_Rep2_S56
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR2_Rep1_S7
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR2_Rep2_S62
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR3_Rep1_S13
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR3_Rep2_S68
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR4_Rep1_S19
jesse-engreitz:auxiliary_CRUDO_MYC-Auxin0hrs-FF1-BinA-PCR4_Rep2_S74

Jesse Engreitz, Stanford

IGVFDS5951VSHM

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI2212HXUF