{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS1172UDGN/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS1172UDGN",
"aliases": [
"jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep4-BinB_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM2245JSDZ](/in-vitro-systems/IGVFSM2245JSDZ/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM2245JSDZ/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS1172UDGN](/measurement-sets/IGVFDS1172UDGN/) has sequence file(s) [IGVFFI3640YJSS](/sequence-files/IGVFFI3640YJSS/), [IGVFFI8771FLNW](/sequence-files/IGVFFI8771FLNW/), [IGVFFI3612MOAU](/sequence-files/IGVFFI3612MOAU/), [IGVFFI2026RHMG](/sequence-files/IGVFFI2026RHMG/), [IGVFFI8198ZIIB](/sequence-files/IGVFFI8198ZIIB/), [IGVFFI3523ULQE](/sequence-files/IGVFFI3523ULQE/), [IGVFFI6770DQYL](/sequence-files/IGVFFI6770DQYL/), [IGVFFI0333BUMF](/sequence-files/IGVFFI0333BUMF/), [IGVFFI4488IECY](/sequence-files/IGVFFI4488IECY/), [IGVFFI6287TGAF](/sequence-files/IGVFFI6287TGAF/), [IGVFFI8624GIYY](/sequence-files/IGVFFI8624GIYY/), [IGVFFI7590PJPJ](/sequence-files/IGVFFI7590PJPJ/), [IGVFFI5808RXES](/sequence-files/IGVFFI5808RXES/), [IGVFFI6962NKZS](/sequence-files/IGVFFI6962NKZS/), [IGVFFI7353RBVY](/sequence-files/IGVFFI7353RBVY/), [IGVFFI1416BSKI](/sequence-files/IGVFFI1416BSKI/), [IGVFFI7203TDLW](/sequence-files/IGVFFI7203TDLW/), [IGVFFI6307KOIB](/sequence-files/IGVFFI6307KOIB/), [IGVFFI0564DKNZ](/sequence-files/IGVFFI0564DKNZ/), [IGVFFI9572YJIK](/sequence-files/IGVFFI9572YJIK/), [IGVFFI9016DIMT](/sequence-files/IGVFFI9016DIMT/), [IGVFFI8402LRYY](/sequence-files/IGVFFI8402LRYY/), [IGVFFI2702PLXY](/sequence-files/IGVFFI2702PLXY/), [IGVFFI6255DYQD](/sequence-files/IGVFFI6255DYQD/), [IGVFFI5098OFWN](/sequence-files/IGVFFI5098OFWN/), [IGVFFI6181JWBD](/sequence-files/IGVFFI6181JWBD/), [IGVFFI3731QSTU](/sequence-files/IGVFFI3731QSTU/), [IGVFFI4515EDPH](/sequence-files/IGVFFI4515EDPH/), [IGVFFI0615PMXK](/sequence-files/IGVFFI0615PMXK/), [IGVFFI5576VURX](/sequence-files/IGVFFI5576VURX/), [IGVFFI9193XIUQ](/sequence-files/IGVFFI9193XIUQ/), [IGVFFI6167BHUI](/sequence-files/IGVFFI6167BHUI/), [IGVFFI7277RRGS](/sequence-files/IGVFFI7277RRGS/), [IGVFFI1650GDGZ](/sequence-files/IGVFFI1650GDGZ/), [IGVFFI1336UIDV](/sequence-files/IGVFFI1336UIDV/), [IGVFFI3083HLUY](/sequence-files/IGVFFI3083HLUY/), [IGVFFI3885EHXH](/sequence-files/IGVFFI3885EHXH/), [IGVFFI3943UOHX](/sequence-files/IGVFFI3943UOHX/), [IGVFFI8329CJTH](/sequence-files/IGVFFI8329CJTH/), [IGVFFI9818WNAB](/sequence-files/IGVFFI9818WNAB/), [IGVFFI6372RCHG](/sequence-files/IGVFFI6372RCHG/), [IGVFFI8421BWDD](/sequence-files/IGVFFI8421BWDD/), [IGVFFI9705KMKE](/sequence-files/IGVFFI9705KMKE/), [IGVFFI0636VCKN](/sequence-files/IGVFFI0636VCKN/), [IGVFFI6700YJXZ](/sequence-files/IGVFFI6700YJXZ/), [IGVFFI6444PXQP](/sequence-files/IGVFFI6444PXQP/), [IGVFFI5136DVDD](/sequence-files/IGVFFI5136DVDD/), [IGVFFI6952ZANA](/sequence-files/IGVFFI6952ZANA/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS1172UDGN/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS1172UDGN](/measurement-sets/IGVFDS1172UDGN/) has sequence file(s): [IGVFFI3640YJSS](/sequence-files/IGVFFI3640YJSS/), [IGVFFI8771FLNW](/sequence-files/IGVFFI8771FLNW/), [IGVFFI3612MOAU](/sequence-files/IGVFFI3612MOAU/), [IGVFFI2026RHMG](/sequence-files/IGVFFI2026RHMG/), [IGVFFI8198ZIIB](/sequence-files/IGVFFI8198ZIIB/), [IGVFFI3523ULQE](/sequence-files/IGVFFI3523ULQE/), [IGVFFI6770DQYL](/sequence-files/IGVFFI6770DQYL/), [IGVFFI0333BUMF](/sequence-files/IGVFFI0333BUMF/), [IGVFFI4488IECY](/sequence-files/IGVFFI4488IECY/), [IGVFFI6287TGAF](/sequence-files/IGVFFI6287TGAF/), [IGVFFI8624GIYY](/sequence-files/IGVFFI8624GIYY/), [IGVFFI7590PJPJ](/sequence-files/IGVFFI7590PJPJ/), [IGVFFI5808RXES](/sequence-files/IGVFFI5808RXES/), [IGVFFI6962NKZS](/sequence-files/IGVFFI6962NKZS/), [IGVFFI7353RBVY](/sequence-files/IGVFFI7353RBVY/), [IGVFFI1416BSKI](/sequence-files/IGVFFI1416BSKI/), [IGVFFI7203TDLW](/sequence-files/IGVFFI7203TDLW/), [IGVFFI6307KOIB](/sequence-files/IGVFFI6307KOIB/), [IGVFFI0564DKNZ](/sequence-files/IGVFFI0564DKNZ/), [IGVFFI9572YJIK](/sequence-files/IGVFFI9572YJIK/), [IGVFFI9016DIMT](/sequence-files/IGVFFI9016DIMT/), [IGVFFI8402LRYY](/sequence-files/IGVFFI8402LRYY/), [IGVFFI2702PLXY](/sequence-files/IGVFFI2702PLXY/), [IGVFFI6255DYQD](/sequence-files/IGVFFI6255DYQD/), [IGVFFI5098OFWN](/sequence-files/IGVFFI5098OFWN/), [IGVFFI6181JWBD](/sequence-files/IGVFFI6181JWBD/), [IGVFFI3731QSTU](/sequence-files/IGVFFI3731QSTU/), [IGVFFI4515EDPH](/sequence-files/IGVFFI4515EDPH/), [IGVFFI0615PMXK](/sequence-files/IGVFFI0615PMXK/), [IGVFFI5576VURX](/sequence-files/IGVFFI5576VURX/), [IGVFFI9193XIUQ](/sequence-files/IGVFFI9193XIUQ/), [IGVFFI6167BHUI](/sequence-files/IGVFFI6167BHUI/), [IGVFFI7277RRGS](/sequence-files/IGVFFI7277RRGS/), [IGVFFI1650GDGZ](/sequence-files/IGVFFI1650GDGZ/), [IGVFFI1336UIDV](/sequence-files/IGVFFI1336UIDV/), [IGVFFI3083HLUY](/sequence-files/IGVFFI3083HLUY/), [IGVFFI3885EHXH](/sequence-files/IGVFFI3885EHXH/), [IGVFFI3943UOHX](/sequence-files/IGVFFI3943UOHX/), [IGVFFI8329CJTH](/sequence-files/IGVFFI8329CJTH/), [IGVFFI9818WNAB](/sequence-files/IGVFFI9818WNAB/), [IGVFFI6372RCHG](/sequence-files/IGVFFI6372RCHG/), [IGVFFI8421BWDD](/sequence-files/IGVFFI8421BWDD/), [IGVFFI9705KMKE](/sequence-files/IGVFFI9705KMKE/), [IGVFFI0636VCKN](/sequence-files/IGVFFI0636VCKN/), [IGVFFI6700YJXZ](/sequence-files/IGVFFI6700YJXZ/), [IGVFFI6444PXQP](/sequence-files/IGVFFI6444PXQP/), [IGVFFI5136DVDD](/sequence-files/IGVFFI5136DVDD/), [IGVFFI6952ZANA](/sequence-files/IGVFFI6952ZANA/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS1172UDGN/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS1172UDGN](/measurement-sets/IGVFDS1172UDGN/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS1172UDGN/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
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],
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}
],
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{
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"aliases": [
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],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
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},
"submitted_files_timestamp": "2023-12-13T09:52:38.769473+00:00",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
"targeted_genes": [
{
"@id": "/genes/ENSG00000108179/",
"geneid": "ENSG00000108179",
"name": "peptidylprolyl isomerase F",
"status": "released",
"symbol": "PPIF",
"synonyms": [
"rotamase F",
"CYP3",
"cyclophilin F",
"mitochondrial cyclophilin",
"peptidyl-prolyl cis-trans isomerase, mitochondrial",
"CypD",
"Cyp-D",
"cyclophilin 3",
"cyclophilin D",
"CyP-M",
"peptidyl-prolyl cis-trans isomerase F, mitochondrial",
"PPIase F"
]
}
],
"uuid": "12e17935-c33d-464f-85bb-36b5016bc7e4"
}