{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS1069GOGI/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS1069GOGI",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinC_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM5829EVGX](/in-vitro-systems/IGVFSM5829EVGX/) has in progress subobject construct library set [IGVFDS6377KNCH](/construct-library-sets/IGVFDS6377KNCH/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM5829EVGX/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS1069GOGI](/measurement-sets/IGVFDS1069GOGI/) has sequence file(s) [IGVFFI9728ZRAO](/sequence-files/IGVFFI9728ZRAO/), [IGVFFI1460HVUS](/sequence-files/IGVFFI1460HVUS/), [IGVFFI9306SZIE](/sequence-files/IGVFFI9306SZIE/), [IGVFFI8737PUPF](/sequence-files/IGVFFI8737PUPF/), [IGVFFI2250TASX](/sequence-files/IGVFFI2250TASX/), [IGVFFI2367HZCG](/sequence-files/IGVFFI2367HZCG/), [IGVFFI6540JCCM](/sequence-files/IGVFFI6540JCCM/), [IGVFFI6810XKIW](/sequence-files/IGVFFI6810XKIW/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS1069GOGI/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS1069GOGI](/measurement-sets/IGVFDS1069GOGI/) has sequence file(s): [IGVFFI9728ZRAO](/sequence-files/IGVFFI9728ZRAO/), [IGVFFI1460HVUS](/sequence-files/IGVFFI1460HVUS/), [IGVFFI9306SZIE](/sequence-files/IGVFFI9306SZIE/), [IGVFFI8737PUPF](/sequence-files/IGVFFI8737PUPF/), [IGVFFI2250TASX](/sequence-files/IGVFFI2250TASX/), [IGVFFI2367HZCG](/sequence-files/IGVFFI2367HZCG/), [IGVFFI6540JCCM](/sequence-files/IGVFFI6540JCCM/), [IGVFFI6810XKIW](/sequence-files/IGVFFI6810XKIW/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS1069GOGI/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS1069GOGI](/measurement-sets/IGVFDS1069GOGI/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS1069GOGI/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"control_file_sets": [
{
"@id": "/measurement-sets/IGVFDS9477ARWI/",
"accession": "IGVFDS9477ARWI",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing",
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing"
],
"status": "released"
}
],
"control_for": [],
"creation_timestamp": "2023-11-30T22:48:28.201661+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO1080XFGV/",
"accession": "IGVFDO1080XFGV",
"aliases": [
"igvf:donor_of_Jurkat"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
"@id": "/sequence-files/IGVFFI9728ZRAO/",
"accession": "IGVFFI9728ZRAO",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinC-PCRrep2_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:09:26.762563+00:00",
"file_format": "fastq",
"file_size": 8143146,
"href": "/sequence-files/IGVFFI9728ZRAO/@@download/IGVFFI9728ZRAO.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/e8881275-3544-49f0-a568-7afe6e86740a/IGVFFI9728ZRAO.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S18-BioRep3-FFRep2-BinC-PCRrep2_S207_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI1460HVUS/",
"accession": "IGVFFI1460HVUS",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinC-PCRrep2_I1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:26:18.562086+00:00",
"file_format": "fastq",
"file_size": 3669494,
"href": "/sequence-files/IGVFFI1460HVUS/@@download/IGVFFI1460HVUS.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/17f35b99-56eb-498b-9b48-b1e8e4a0917e/IGVFFI1460HVUS.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S18-BioRep3-FFRep2-BinC-PCRrep2_S207_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI9306SZIE/",
"accession": "IGVFFI9306SZIE",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinC-PCRrep1_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:01:12.622031+00:00",
"file_format": "fastq",
"file_size": 3130031,
"href": "/sequence-files/IGVFFI9306SZIE/@@download/IGVFFI9306SZIE.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/95006399-0d09-4390-9b5c-dd3ca685b937/IGVFFI9306SZIE.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S18-BioRep3-FFRep2-BinC-PCRrep1_S150_I2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8737PUPF/",
"accession": "IGVFFI8737PUPF",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinC-PCRrep2_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:34:31.794055+00:00",
"file_format": "fastq",
"file_size": 3433259,
"href": "/sequence-files/IGVFFI8737PUPF/@@download/IGVFFI8737PUPF.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/c20b6ee0-fd05-4828-af58-6b404266ab6f/IGVFFI8737PUPF.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S18-BioRep3-FFRep2-BinC-PCRrep2_S207_I2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI2250TASX/",
"accession": "IGVFFI2250TASX",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinC-PCRrep2_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T20:17:54.663521+00:00",
"file_format": "fastq",
"file_size": 15138382,
"href": "/sequence-files/IGVFFI2250TASX/@@download/IGVFFI2250TASX.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/ad803041-1cbf-4c6e-a4a8-8eb8debc399a/IGVFFI2250TASX.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S18-BioRep3-FFRep2-BinC-PCRrep2_S207_R2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI2367HZCG/",
"accession": "IGVFFI2367HZCG",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinC-PCRrep1_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:44:43.296670+00:00",
"file_format": "fastq",
"file_size": 13898325,
"href": "/sequence-files/IGVFFI2367HZCG/@@download/IGVFFI2367HZCG.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/a7b4620a-7406-4e5c-9d7c-6d82b35b440c/IGVFFI2367HZCG.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S18-BioRep3-FFRep2-BinC-PCRrep1_S150_R2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI6540JCCM/",
"accession": "IGVFFI6540JCCM",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinC-PCRrep1_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:36:15.668449+00:00",
"file_format": "fastq",
"file_size": 7561080,
"href": "/sequence-files/IGVFFI6540JCCM/@@download/IGVFFI6540JCCM.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/5074b023-edd3-4c28-b5cf-c3deb334ebf9/IGVFFI6540JCCM.fastq.gz",
"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S18-BioRep3-FFRep2-BinC-PCRrep1_S150_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI6810XKIW/",
"accession": "IGVFFI6810XKIW",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinC-PCRrep1_I1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:53:05.294322+00:00",
"file_format": "fastq",
"file_size": 3229649,
"href": "/sequence-files/IGVFFI6810XKIW/@@download/IGVFFI6810XKIW.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/fd0732d9-e0e6-48cc-ba3f-8716869caaff/IGVFFI6810XKIW.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF2_S18-BioRep3-FFRep2-BinC-PCRrep1_S150_I1_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS1863TJOE/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
"preferred_assay_title": "Variant-EFFECTS",
"protocols": [
"https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
],
"release_timestamp": "2023-12-20T23:10:55.905152+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM5829EVGX/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM5829EVGX",
"aliases": [
"jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-BioRep3-FFrep2-BinC"
],
"classifications": [
"cell line"
],
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS6377KNCH/",
"accession": "IGVFDS6377KNCH",
"file_set_type": "guide library",
"status": "in progress",
"summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
}
],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:Jurkat"
],
"status": "released",
"summary": "Jurkat",
"term_name": "Jurkat"
}
],
"status": "released",
"summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 35-45% expression of PPIF) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
"taxa": "Homo sapiens",
"treatments": [
{
"@id": "/treatments/df02c0d4-f796-4f77-bfbc-388cdbfcc615/",
"status": "released",
"treatment_term_name": "T-cell surface glycoprotein CD3 gamma chain"
},
{
"@id": "/treatments/42b3c61d-38de-4bd2-bb39-33c125a417cb/",
"status": "released",
"treatment_term_name": "phorbol 13-acetate 12-myristate"
}
]
}
],
"schema_version": "23",
"status": "released",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_files_timestamp": "2023-12-05T19:36:15.668449+00:00",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
"targeted_genes": [
{
"@id": "/genes/ENSG00000108179/",
"geneid": "ENSG00000108179",
"name": "peptidylprolyl isomerase F",
"status": "released",
"symbol": "PPIF",
"synonyms": [
"rotamase F",
"CYP3",
"cyclophilin F",
"mitochondrial cyclophilin",
"peptidyl-prolyl cis-trans isomerase, mitochondrial",
"CypD",
"Cyp-D",
"cyclophilin 3",
"cyclophilin D",
"CyP-M",
"peptidyl-prolyl cis-trans isomerase F, mitochondrial",
"PPIase F"
]
}
],
"uuid": "dbaeb7b4-e521-4e92-b782-9daa5d441699"
}