IGVFDS0934SYRQ

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS0934SYRQ/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS0934SYRQ",
    "aliases": [
        "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep1-BinA_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS0934SYRQ](/measurement-sets/IGVFDS0934SYRQ/) has sequence file(s) [IGVFFI6739SQTK](/sequence-files/IGVFFI6739SQTK/), [IGVFFI0286TBHI](/sequence-files/IGVFFI0286TBHI/), [IGVFFI0574EAGF](/sequence-files/IGVFFI0574EAGF/), [IGVFFI3563PTOE](/sequence-files/IGVFFI3563PTOE/), [IGVFFI2679KRUC](/sequence-files/IGVFFI2679KRUC/), [IGVFFI4558IXGW](/sequence-files/IGVFFI4558IXGW/), [IGVFFI0791CPNC](/sequence-files/IGVFFI0791CPNC/), [IGVFFI2557NBLU](/sequence-files/IGVFFI2557NBLU/), [IGVFFI6943HUNA](/sequence-files/IGVFFI6943HUNA/), [IGVFFI3723KXEY](/sequence-files/IGVFFI3723KXEY/), [IGVFFI6755EXPU](/sequence-files/IGVFFI6755EXPU/), [IGVFFI0170IIJR](/sequence-files/IGVFFI0170IIJR/), [IGVFFI9883GWFX](/sequence-files/IGVFFI9883GWFX/), [IGVFFI8650UVHT](/sequence-files/IGVFFI8650UVHT/), [IGVFFI9502TRDQ](/sequence-files/IGVFFI9502TRDQ/), [IGVFFI4454MJUH](/sequence-files/IGVFFI4454MJUH/), [IGVFFI0860GIXW](/sequence-files/IGVFFI0860GIXW/), [IGVFFI4597JJEN](/sequence-files/IGVFFI4597JJEN/), [IGVFFI9533KEOA](/sequence-files/IGVFFI9533KEOA/), [IGVFFI9311LLRP](/sequence-files/IGVFFI9311LLRP/), [IGVFFI3098AJAP](/sequence-files/IGVFFI3098AJAP/), [IGVFFI8577IGPG](/sequence-files/IGVFFI8577IGPG/), [IGVFFI6256ANPJ](/sequence-files/IGVFFI6256ANPJ/), [IGVFFI3903MZBT](/sequence-files/IGVFFI3903MZBT/), [IGVFFI9452RFBC](/sequence-files/IGVFFI9452RFBC/), [IGVFFI9105DOIH](/sequence-files/IGVFFI9105DOIH/), [IGVFFI3113CFJM](/sequence-files/IGVFFI3113CFJM/), [IGVFFI9464KGND](/sequence-files/IGVFFI9464KGND/), [IGVFFI7100QPVK](/sequence-files/IGVFFI7100QPVK/), [IGVFFI9145JWRO](/sequence-files/IGVFFI9145JWRO/), [IGVFFI2143FXJB](/sequence-files/IGVFFI2143FXJB/), [IGVFFI5003UCQS](/sequence-files/IGVFFI5003UCQS/), [IGVFFI1929ZUTI](/sequence-files/IGVFFI1929ZUTI/), [IGVFFI0100YQYA](/sequence-files/IGVFFI0100YQYA/), [IGVFFI2032BGYI](/sequence-files/IGVFFI2032BGYI/), [IGVFFI4508NSLN](/sequence-files/IGVFFI4508NSLN/), [IGVFFI5287VSRE](/sequence-files/IGVFFI5287VSRE/), [IGVFFI7367KTLU](/sequence-files/IGVFFI7367KTLU/), [IGVFFI8126JGXQ](/sequence-files/IGVFFI8126JGXQ/), [IGVFFI5037ULUU](/sequence-files/IGVFFI5037ULUU/), [IGVFFI6158MJIN](/sequence-files/IGVFFI6158MJIN/), [IGVFFI8544WVQG](/sequence-files/IGVFFI8544WVQG/), [IGVFFI0315TLGN](/sequence-files/IGVFFI0315TLGN/), [IGVFFI1044WEIN](/sequence-files/IGVFFI1044WEIN/), [IGVFFI0076FIBX](/sequence-files/IGVFFI0076FIBX/), [IGVFFI9558AYHA](/sequence-files/IGVFFI9558AYHA/), [IGVFFI2762JLDW](/sequence-files/IGVFFI2762JLDW/), [IGVFFI4823HGER](/sequence-files/IGVFFI4823HGER/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS0934SYRQ/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS0934SYRQ](/measurement-sets/IGVFDS0934SYRQ/) has sequence file(s): [IGVFFI6739SQTK](/sequence-files/IGVFFI6739SQTK/), [IGVFFI0286TBHI](/sequence-files/IGVFFI0286TBHI/), [IGVFFI0574EAGF](/sequence-files/IGVFFI0574EAGF/), [IGVFFI3563PTOE](/sequence-files/IGVFFI3563PTOE/), [IGVFFI2679KRUC](/sequence-files/IGVFFI2679KRUC/), [IGVFFI4558IXGW](/sequence-files/IGVFFI4558IXGW/), [IGVFFI0791CPNC](/sequence-files/IGVFFI0791CPNC/), [IGVFFI2557NBLU](/sequence-files/IGVFFI2557NBLU/), [IGVFFI6943HUNA](/sequence-files/IGVFFI6943HUNA/), [IGVFFI3723KXEY](/sequence-files/IGVFFI3723KXEY/), [IGVFFI6755EXPU](/sequence-files/IGVFFI6755EXPU/), [IGVFFI0170IIJR](/sequence-files/IGVFFI0170IIJR/), [IGVFFI9883GWFX](/sequence-files/IGVFFI9883GWFX/), [IGVFFI8650UVHT](/sequence-files/IGVFFI8650UVHT/), [IGVFFI9502TRDQ](/sequence-files/IGVFFI9502TRDQ/), [IGVFFI4454MJUH](/sequence-files/IGVFFI4454MJUH/), [IGVFFI0860GIXW](/sequence-files/IGVFFI0860GIXW/), [IGVFFI4597JJEN](/sequence-files/IGVFFI4597JJEN/), [IGVFFI9533KEOA](/sequence-files/IGVFFI9533KEOA/), [IGVFFI9311LLRP](/sequence-files/IGVFFI9311LLRP/), [IGVFFI3098AJAP](/sequence-files/IGVFFI3098AJAP/), [IGVFFI8577IGPG](/sequence-files/IGVFFI8577IGPG/), [IGVFFI6256ANPJ](/sequence-files/IGVFFI6256ANPJ/), [IGVFFI3903MZBT](/sequence-files/IGVFFI3903MZBT/), [IGVFFI9452RFBC](/sequence-files/IGVFFI9452RFBC/), [IGVFFI9105DOIH](/sequence-files/IGVFFI9105DOIH/), [IGVFFI3113CFJM](/sequence-files/IGVFFI3113CFJM/), [IGVFFI9464KGND](/sequence-files/IGVFFI9464KGND/), [IGVFFI7100QPVK](/sequence-files/IGVFFI7100QPVK/), [IGVFFI9145JWRO](/sequence-files/IGVFFI9145JWRO/), [IGVFFI2143FXJB](/sequence-files/IGVFFI2143FXJB/), [IGVFFI5003UCQS](/sequence-files/IGVFFI5003UCQS/), [IGVFFI1929ZUTI](/sequence-files/IGVFFI1929ZUTI/), [IGVFFI0100YQYA](/sequence-files/IGVFFI0100YQYA/), [IGVFFI2032BGYI](/sequence-files/IGVFFI2032BGYI/), [IGVFFI4508NSLN](/sequence-files/IGVFFI4508NSLN/), [IGVFFI5287VSRE](/sequence-files/IGVFFI5287VSRE/), [IGVFFI7367KTLU](/sequence-files/IGVFFI7367KTLU/), [IGVFFI8126JGXQ](/sequence-files/IGVFFI8126JGXQ/), [IGVFFI5037ULUU](/sequence-files/IGVFFI5037ULUU/), [IGVFFI6158MJIN](/sequence-files/IGVFFI6158MJIN/), [IGVFFI8544WVQG](/sequence-files/IGVFFI8544WVQG/), [IGVFFI0315TLGN](/sequence-files/IGVFFI0315TLGN/), [IGVFFI1044WEIN](/sequence-files/IGVFFI1044WEIN/), [IGVFFI0076FIBX](/sequence-files/IGVFFI0076FIBX/), [IGVFFI9558AYHA](/sequence-files/IGVFFI9558AYHA/), [IGVFFI2762JLDW](/sequence-files/IGVFFI2762JLDW/), [IGVFFI4823HGER](/sequence-files/IGVFFI4823HGER/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS0934SYRQ/"
            },
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM1176SLYJ](/in-vitro-systems/IGVFSM1176SLYJ/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM1176SLYJ/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS0934SYRQ](/measurement-sets/IGVFDS0934SYRQ/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS0934SYRQ/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
    "control_file_sets": [
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            "@id": "/measurement-sets/IGVFDS4869FXYY/",
            "accession": "IGVFDS4869FXYY",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:48:09.888597+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep2-FFRep1_BinA-PCRrep8_S200_R1_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS6299IJHG/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:08:48.897838+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM1176SLYJ/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM1176SLYJ",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep1-BinA"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "released",
            "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T10:00:25.360792+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "5d35da39-bc37-42ed-9aba-28ac4f2b6e43"
}