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IGVFDS0886UBIB

released
File Set Type
experimental data
Summary
CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in many genes
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
  • Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library
Protocols
Aliases
charles-gersbach:hPA-measurement-set-perturbseq-10xlane1-GEX
Attribution
Charles Gersbach, Duke

Sequencing Results (Illumina)

4 items
Read Names
fastq
reads
R1
4
Read 1
2
3.4 GB
Charles Gersbach, Duke
validated
fastq
reads
R2
4
Read 2
2
8.3 GB
Charles Gersbach, Duke
validated
fastq
reads
R1
4
Read 1
1
3.4 GB
Charles Gersbach, Duke
validated
fastq
reads
R2
4
Read 2
1
8.3 GB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
PrimaryCell
Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:hPA-donor-34969
unspecified
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting transcription start sites in many genes

Auxiliary Datasets

1 item
Accession
Type
Summary
Files
Aliases
Lab
AuxiliarySet
gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in many genes
  • charles-gersbach:hPA-auxiliary-set-perturbseq-10xlane1-CRISPR
Charles Gersbach, Duke