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IGVFDS0870PAHS

released
File Set Type
experimental data
Summary
CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in GATA3
Assay Term
bulk RNA-seq assay
Sample Summaries
  • CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Protocols
Aliases
charles-gersbach:cd8-tcells-rna-seq-cas-ko-gata3-rep1
Publications

Sequencing Results (Illumina)

2 items
Read Names
fastq
reads
R1
1
1
2.4 GB
Charles Gersbach, Duke
validated
fastq
reads
R2
1
1
2.5 GB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-12
unspecified
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting transcription start sites in GATA3

Control File Sets

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
CRISPR knockout RNA-seq integrating a non-targeting guide (sgRNA) library
  • charles-gersbach:cd8-tcells-rna-seq-cas-ko-nt-rep1
Charles Gersbach, Duke

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
knockout RNA-seq: fold change over control, transcript quantifications, transcriptome alignments
  • charles-gersbach:cd8-tcells-analysis-set-rna-seq-cas-ko-gata3
Charles Gersbach, Duke

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke