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IGVFDS0707NWZU

released
File Set Type
experimental data
Control Type
low FACS signal
Summary
low FACS signal CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Protocols
Aliases
charles-gersbach:jurkats-measurement-set-crispra-vp64-il2ra-low-bin-rep2
Publications
Attribution
Charles Gersbach, Duke

Sequencing Results (Illumina)

1 item
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Read Names
Associated seqspec Files
Sequence Specification Document
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
R1
2
000000000-JFKPJ
1
27 MB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_Jurkat
male
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting transcription start sites in IL2RA

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
CRISPR activation FACS screen targeting IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA with low FACS signal control
  • charles-gersbach:jurkats-analysis-set-crispra-vp64-il2ra
Charles Gersbach, Duke

File Sets Controlled by This Measurement Set

1 item
Accession
Type
Summary
Aliases
Lab
MeasurementSet
CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
  • charles-gersbach:jurkats-measurement-set-crispra-vp64-il2ra-high-bin-rep2
Charles Gersbach, Duke