IGVFDS0674KOZU

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

ITPRID2

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Construct Library Sets

IGVFDS5413RKIA guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinF-PCR1-Rep1_S6

jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinF-PCR2-Rep1_S12

jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinF-PCR3-Rep1_S60

jesse-engreitz:measurement_CRUDO_SSFA2-Auxin0hrs-FF1-BinF-PCR4-Rep1_S66

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4326ZGNE

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS1369IMLU

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI1019WGYS, IGVFFI8263MWWS, IGVFFI3575WNTZ, IGVFFI9696NSTM

jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF1-BinF-PCR1-Rep1_S6
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF1-BinF-PCR2-Rep1_S12
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF1-BinF-PCR3-Rep1_S60
jesse-engreitz:auxiliary_CRUDO_SSFA2-Auxin0hrs-FF1-BinF-PCR4-Rep1_S66

Jesse Engreitz, Stanford

IGVFDS1546MEZJ

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI1231XMQS