{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS0649NZHX/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS0649NZHX",
"aliases": [
"jesse-engreitz:230523pPPIFEnformer-THP1-Amp_PPIF_promoter-BioRep1-FFrep1-BinB_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM6784MZOT](/in-vitro-systems/IGVFSM6784MZOT/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM6784MZOT/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS0649NZHX](/measurement-sets/IGVFDS0649NZHX/) has sequence file(s) [IGVFFI5539BOBA](/sequence-files/IGVFFI5539BOBA/), [IGVFFI6944IORM](/sequence-files/IGVFFI6944IORM/), [IGVFFI7994RXZI](/sequence-files/IGVFFI7994RXZI/), [IGVFFI3070BYXY](/sequence-files/IGVFFI3070BYXY/), [IGVFFI3837WDLK](/sequence-files/IGVFFI3837WDLK/), [IGVFFI4199KVLO](/sequence-files/IGVFFI4199KVLO/), [IGVFFI8355YJTF](/sequence-files/IGVFFI8355YJTF/), [IGVFFI9143KWLU](/sequence-files/IGVFFI9143KWLU/), [IGVFFI8836QTRR](/sequence-files/IGVFFI8836QTRR/), [IGVFFI7111CSTT](/sequence-files/IGVFFI7111CSTT/), [IGVFFI1184CQVO](/sequence-files/IGVFFI1184CQVO/), [IGVFFI0396DSME](/sequence-files/IGVFFI0396DSME/), [IGVFFI8609TIHW](/sequence-files/IGVFFI8609TIHW/), [IGVFFI0067VSPN](/sequence-files/IGVFFI0067VSPN/), [IGVFFI3761RNPN](/sequence-files/IGVFFI3761RNPN/), [IGVFFI4693FJTS](/sequence-files/IGVFFI4693FJTS/), [IGVFFI3063NIHT](/sequence-files/IGVFFI3063NIHT/), [IGVFFI8959ACSK](/sequence-files/IGVFFI8959ACSK/), [IGVFFI4516KFGA](/sequence-files/IGVFFI4516KFGA/), [IGVFFI6839COMN](/sequence-files/IGVFFI6839COMN/), [IGVFFI4423OXOL](/sequence-files/IGVFFI4423OXOL/), [IGVFFI3289BORC](/sequence-files/IGVFFI3289BORC/), [IGVFFI6736WIXV](/sequence-files/IGVFFI6736WIXV/), [IGVFFI8731DWKI](/sequence-files/IGVFFI8731DWKI/), [IGVFFI6525HRBA](/sequence-files/IGVFFI6525HRBA/), [IGVFFI9503OUYA](/sequence-files/IGVFFI9503OUYA/), [IGVFFI3455ITYP](/sequence-files/IGVFFI3455ITYP/), [IGVFFI6164VNIA](/sequence-files/IGVFFI6164VNIA/), [IGVFFI8902DSVE](/sequence-files/IGVFFI8902DSVE/), [IGVFFI9339SLDF](/sequence-files/IGVFFI9339SLDF/), [IGVFFI0624KKKY](/sequence-files/IGVFFI0624KKKY/), [IGVFFI8586BVYX](/sequence-files/IGVFFI8586BVYX/), [IGVFFI1337EMZN](/sequence-files/IGVFFI1337EMZN/), [IGVFFI9823KTAK](/sequence-files/IGVFFI9823KTAK/), [IGVFFI3654UMQO](/sequence-files/IGVFFI3654UMQO/), [IGVFFI9123LEUC](/sequence-files/IGVFFI9123LEUC/), [IGVFFI8889WEQZ](/sequence-files/IGVFFI8889WEQZ/), [IGVFFI3066LCET](/sequence-files/IGVFFI3066LCET/), [IGVFFI0941MYBB](/sequence-files/IGVFFI0941MYBB/), [IGVFFI2236HMRF](/sequence-files/IGVFFI2236HMRF/), [IGVFFI8619DYIT](/sequence-files/IGVFFI8619DYIT/), [IGVFFI4388AVGJ](/sequence-files/IGVFFI4388AVGJ/), [IGVFFI8239HVHU](/sequence-files/IGVFFI8239HVHU/), [IGVFFI0116NIQJ](/sequence-files/IGVFFI0116NIQJ/), [IGVFFI1929VUST](/sequence-files/IGVFFI1929VUST/), [IGVFFI0381FPNM](/sequence-files/IGVFFI0381FPNM/), [IGVFFI3654HJBV](/sequence-files/IGVFFI3654HJBV/), [IGVFFI1753SSFD](/sequence-files/IGVFFI1753SSFD/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS0649NZHX/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS0649NZHX](/measurement-sets/IGVFDS0649NZHX/) has sequence file(s): [IGVFFI5539BOBA](/sequence-files/IGVFFI5539BOBA/), [IGVFFI6944IORM](/sequence-files/IGVFFI6944IORM/), [IGVFFI7994RXZI](/sequence-files/IGVFFI7994RXZI/), [IGVFFI3070BYXY](/sequence-files/IGVFFI3070BYXY/), [IGVFFI3837WDLK](/sequence-files/IGVFFI3837WDLK/), [IGVFFI4199KVLO](/sequence-files/IGVFFI4199KVLO/), [IGVFFI8355YJTF](/sequence-files/IGVFFI8355YJTF/), [IGVFFI9143KWLU](/sequence-files/IGVFFI9143KWLU/), [IGVFFI8836QTRR](/sequence-files/IGVFFI8836QTRR/), [IGVFFI7111CSTT](/sequence-files/IGVFFI7111CSTT/), [IGVFFI1184CQVO](/sequence-files/IGVFFI1184CQVO/), [IGVFFI0396DSME](/sequence-files/IGVFFI0396DSME/), [IGVFFI8609TIHW](/sequence-files/IGVFFI8609TIHW/), [IGVFFI0067VSPN](/sequence-files/IGVFFI0067VSPN/), [IGVFFI3761RNPN](/sequence-files/IGVFFI3761RNPN/), [IGVFFI4693FJTS](/sequence-files/IGVFFI4693FJTS/), [IGVFFI3063NIHT](/sequence-files/IGVFFI3063NIHT/), [IGVFFI8959ACSK](/sequence-files/IGVFFI8959ACSK/), [IGVFFI4516KFGA](/sequence-files/IGVFFI4516KFGA/), [IGVFFI6839COMN](/sequence-files/IGVFFI6839COMN/), [IGVFFI4423OXOL](/sequence-files/IGVFFI4423OXOL/), [IGVFFI3289BORC](/sequence-files/IGVFFI3289BORC/), [IGVFFI6736WIXV](/sequence-files/IGVFFI6736WIXV/), [IGVFFI8731DWKI](/sequence-files/IGVFFI8731DWKI/), [IGVFFI6525HRBA](/sequence-files/IGVFFI6525HRBA/), [IGVFFI9503OUYA](/sequence-files/IGVFFI9503OUYA/), [IGVFFI3455ITYP](/sequence-files/IGVFFI3455ITYP/), [IGVFFI6164VNIA](/sequence-files/IGVFFI6164VNIA/), [IGVFFI8902DSVE](/sequence-files/IGVFFI8902DSVE/), [IGVFFI9339SLDF](/sequence-files/IGVFFI9339SLDF/), [IGVFFI0624KKKY](/sequence-files/IGVFFI0624KKKY/), [IGVFFI8586BVYX](/sequence-files/IGVFFI8586BVYX/), [IGVFFI1337EMZN](/sequence-files/IGVFFI1337EMZN/), [IGVFFI9823KTAK](/sequence-files/IGVFFI9823KTAK/), [IGVFFI3654UMQO](/sequence-files/IGVFFI3654UMQO/), [IGVFFI9123LEUC](/sequence-files/IGVFFI9123LEUC/), [IGVFFI8889WEQZ](/sequence-files/IGVFFI8889WEQZ/), [IGVFFI3066LCET](/sequence-files/IGVFFI3066LCET/), [IGVFFI0941MYBB](/sequence-files/IGVFFI0941MYBB/), [IGVFFI2236HMRF](/sequence-files/IGVFFI2236HMRF/), [IGVFFI8619DYIT](/sequence-files/IGVFFI8619DYIT/), [IGVFFI4388AVGJ](/sequence-files/IGVFFI4388AVGJ/), [IGVFFI8239HVHU](/sequence-files/IGVFFI8239HVHU/), [IGVFFI0116NIQJ](/sequence-files/IGVFFI0116NIQJ/), [IGVFFI1929VUST](/sequence-files/IGVFFI1929VUST/), [IGVFFI0381FPNM](/sequence-files/IGVFFI0381FPNM/), [IGVFFI3654HJBV](/sequence-files/IGVFFI3654HJBV/), [IGVFFI1753SSFD](/sequence-files/IGVFFI1753SSFD/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS0649NZHX/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS0649NZHX](/measurement-sets/IGVFDS0649NZHX/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS0649NZHX/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
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{
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"accession": "IGVFDS5128CXIS",
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],
"status": "released"
}
],
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"donors": [
{
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"accession": "IGVFDO7359LVPI",
"aliases": [
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],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
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"title": "Michael Montgomery"
},
"submitted_files_timestamp": "2023-12-13T08:16:35.901241+00:00",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
"targeted_genes": [
{
"@id": "/genes/ENSG00000108179/",
"geneid": "ENSG00000108179",
"name": "peptidylprolyl isomerase F",
"status": "released",
"symbol": "PPIF",
"synonyms": [
"rotamase F",
"CYP3",
"cyclophilin F",
"mitochondrial cyclophilin",
"peptidyl-prolyl cis-trans isomerase, mitochondrial",
"CypD",
"Cyp-D",
"cyclophilin 3",
"cyclophilin D",
"CyP-M",
"peptidyl-prolyl cis-trans isomerase F, mitochondrial",
"PPIase F"
]
}
],
"uuid": "a2463aed-33e7-4921-ab0f-f8782a40144c"
}