OBI_0003661

Ian Whaling

in vitro CRISPR screen using flow cytometry

Stanford Center for Connecting DNA Variants to Function and Phenotype

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-10% expression of PPIF) modified with a guide library

THP-1 cell line, male, Homo sapiens modified with a guide library

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 11-20% expression of PPIF) modified with a guide library

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 35-45% expression of PPIF) modified with a guide library

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) modified with a guide library

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 80-90% expression of PPIF) modified with a guide library

THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 90-100% expression of PPIF) modified with a guide library

principal analysis of Variant FlowFISH data

Guide (sgRNA) library targeting sequence variants in a genomic locus

prime editing Variant FlowFISH integrating a guide (sgRNA) library targeting sequence variants in a genomic locus