IGVFDS0595OBOA

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS0595OBOA/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS0595OBOA",
    "aliases": [
        "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep4-BinA_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM4893GPVV](/in-vitro-systems/IGVFSM4893GPVV/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM4893GPVV/"
            },
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS0595OBOA](/measurement-sets/IGVFDS0595OBOA/) has sequence file(s) [IGVFFI7893OAAA](/sequence-files/IGVFFI7893OAAA/), [IGVFFI5932ENNC](/sequence-files/IGVFFI5932ENNC/), [IGVFFI5316QIUX](/sequence-files/IGVFFI5316QIUX/), [IGVFFI2303GWMJ](/sequence-files/IGVFFI2303GWMJ/), [IGVFFI8972TMMD](/sequence-files/IGVFFI8972TMMD/), [IGVFFI5709UQBE](/sequence-files/IGVFFI5709UQBE/), [IGVFFI2465UXYE](/sequence-files/IGVFFI2465UXYE/), [IGVFFI5139YEUZ](/sequence-files/IGVFFI5139YEUZ/), [IGVFFI8067ILTA](/sequence-files/IGVFFI8067ILTA/), [IGVFFI1562DSMD](/sequence-files/IGVFFI1562DSMD/), [IGVFFI0755EFIB](/sequence-files/IGVFFI0755EFIB/), [IGVFFI4551NIEK](/sequence-files/IGVFFI4551NIEK/), [IGVFFI3897LUSJ](/sequence-files/IGVFFI3897LUSJ/), [IGVFFI1490XWNF](/sequence-files/IGVFFI1490XWNF/), [IGVFFI2195KDOM](/sequence-files/IGVFFI2195KDOM/), [IGVFFI8952ZDMH](/sequence-files/IGVFFI8952ZDMH/), [IGVFFI6281HWPB](/sequence-files/IGVFFI6281HWPB/), [IGVFFI9173DQSG](/sequence-files/IGVFFI9173DQSG/), [IGVFFI3737WUMM](/sequence-files/IGVFFI3737WUMM/), [IGVFFI2180YRNU](/sequence-files/IGVFFI2180YRNU/), [IGVFFI8315JDIR](/sequence-files/IGVFFI8315JDIR/), [IGVFFI8504HKOE](/sequence-files/IGVFFI8504HKOE/), [IGVFFI9175PZYF](/sequence-files/IGVFFI9175PZYF/), [IGVFFI7296ABQX](/sequence-files/IGVFFI7296ABQX/), [IGVFFI9402WGFC](/sequence-files/IGVFFI9402WGFC/), [IGVFFI9572VVCE](/sequence-files/IGVFFI9572VVCE/), [IGVFFI0116FTMZ](/sequence-files/IGVFFI0116FTMZ/), [IGVFFI2341QUEX](/sequence-files/IGVFFI2341QUEX/), [IGVFFI1579LBGJ](/sequence-files/IGVFFI1579LBGJ/), [IGVFFI2533RTEK](/sequence-files/IGVFFI2533RTEK/), [IGVFFI0201HMLK](/sequence-files/IGVFFI0201HMLK/), [IGVFFI8898DYAB](/sequence-files/IGVFFI8898DYAB/), [IGVFFI1186MENK](/sequence-files/IGVFFI1186MENK/), [IGVFFI6877PUCW](/sequence-files/IGVFFI6877PUCW/), [IGVFFI4285PDQM](/sequence-files/IGVFFI4285PDQM/), [IGVFFI6409LIKI](/sequence-files/IGVFFI6409LIKI/), [IGVFFI0271OWCU](/sequence-files/IGVFFI0271OWCU/), [IGVFFI9706ZNBC](/sequence-files/IGVFFI9706ZNBC/), [IGVFFI0470AMOB](/sequence-files/IGVFFI0470AMOB/), [IGVFFI0299PRHE](/sequence-files/IGVFFI0299PRHE/), [IGVFFI3515VQWV](/sequence-files/IGVFFI3515VQWV/), [IGVFFI8202YCKI](/sequence-files/IGVFFI8202YCKI/), [IGVFFI6214RUQQ](/sequence-files/IGVFFI6214RUQQ/), [IGVFFI7859JTGD](/sequence-files/IGVFFI7859JTGD/), [IGVFFI2446OVLW](/sequence-files/IGVFFI2446OVLW/), [IGVFFI5248HHSM](/sequence-files/IGVFFI5248HHSM/), [IGVFFI2811QOMX](/sequence-files/IGVFFI2811QOMX/), [IGVFFI8074JBCF](/sequence-files/IGVFFI8074JBCF/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS0595OBOA/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS0595OBOA](/measurement-sets/IGVFDS0595OBOA/) has sequence file(s): [IGVFFI7893OAAA](/sequence-files/IGVFFI7893OAAA/), [IGVFFI5932ENNC](/sequence-files/IGVFFI5932ENNC/), [IGVFFI5316QIUX](/sequence-files/IGVFFI5316QIUX/), [IGVFFI2303GWMJ](/sequence-files/IGVFFI2303GWMJ/), [IGVFFI8972TMMD](/sequence-files/IGVFFI8972TMMD/), [IGVFFI5709UQBE](/sequence-files/IGVFFI5709UQBE/), [IGVFFI2465UXYE](/sequence-files/IGVFFI2465UXYE/), [IGVFFI5139YEUZ](/sequence-files/IGVFFI5139YEUZ/), [IGVFFI8067ILTA](/sequence-files/IGVFFI8067ILTA/), [IGVFFI1562DSMD](/sequence-files/IGVFFI1562DSMD/), [IGVFFI0755EFIB](/sequence-files/IGVFFI0755EFIB/), [IGVFFI4551NIEK](/sequence-files/IGVFFI4551NIEK/), [IGVFFI3897LUSJ](/sequence-files/IGVFFI3897LUSJ/), [IGVFFI1490XWNF](/sequence-files/IGVFFI1490XWNF/), [IGVFFI2195KDOM](/sequence-files/IGVFFI2195KDOM/), [IGVFFI8952ZDMH](/sequence-files/IGVFFI8952ZDMH/), [IGVFFI6281HWPB](/sequence-files/IGVFFI6281HWPB/), [IGVFFI9173DQSG](/sequence-files/IGVFFI9173DQSG/), [IGVFFI3737WUMM](/sequence-files/IGVFFI3737WUMM/), [IGVFFI2180YRNU](/sequence-files/IGVFFI2180YRNU/), [IGVFFI8315JDIR](/sequence-files/IGVFFI8315JDIR/), [IGVFFI8504HKOE](/sequence-files/IGVFFI8504HKOE/), [IGVFFI9175PZYF](/sequence-files/IGVFFI9175PZYF/), [IGVFFI7296ABQX](/sequence-files/IGVFFI7296ABQX/), [IGVFFI9402WGFC](/sequence-files/IGVFFI9402WGFC/), [IGVFFI9572VVCE](/sequence-files/IGVFFI9572VVCE/), [IGVFFI0116FTMZ](/sequence-files/IGVFFI0116FTMZ/), [IGVFFI2341QUEX](/sequence-files/IGVFFI2341QUEX/), [IGVFFI1579LBGJ](/sequence-files/IGVFFI1579LBGJ/), [IGVFFI2533RTEK](/sequence-files/IGVFFI2533RTEK/), [IGVFFI0201HMLK](/sequence-files/IGVFFI0201HMLK/), [IGVFFI8898DYAB](/sequence-files/IGVFFI8898DYAB/), [IGVFFI1186MENK](/sequence-files/IGVFFI1186MENK/), [IGVFFI6877PUCW](/sequence-files/IGVFFI6877PUCW/), [IGVFFI4285PDQM](/sequence-files/IGVFFI4285PDQM/), [IGVFFI6409LIKI](/sequence-files/IGVFFI6409LIKI/), [IGVFFI0271OWCU](/sequence-files/IGVFFI0271OWCU/), [IGVFFI9706ZNBC](/sequence-files/IGVFFI9706ZNBC/), [IGVFFI0470AMOB](/sequence-files/IGVFFI0470AMOB/), [IGVFFI0299PRHE](/sequence-files/IGVFFI0299PRHE/), [IGVFFI3515VQWV](/sequence-files/IGVFFI3515VQWV/), [IGVFFI8202YCKI](/sequence-files/IGVFFI8202YCKI/), [IGVFFI6214RUQQ](/sequence-files/IGVFFI6214RUQQ/), [IGVFFI7859JTGD](/sequence-files/IGVFFI7859JTGD/), [IGVFFI2446OVLW](/sequence-files/IGVFFI2446OVLW/), [IGVFFI5248HHSM](/sequence-files/IGVFFI5248HHSM/), [IGVFFI2811QOMX](/sequence-files/IGVFFI2811QOMX/), [IGVFFI8074JBCF](/sequence-files/IGVFFI8074JBCF/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS0595OBOA/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS0595OBOA](/measurement-sets/IGVFDS0595OBOA/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS0595OBOA/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
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            "@id": "/measurement-sets/IGVFDS4869FXYY/",
            "accession": "IGVFDS4869FXYY",
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                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:48:08.053217+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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        },
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            "@id": "/sequence-files/IGVFFI0755EFIB/",
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            "s3_uri": "s3://igvf-files/2023/12/13/fd2c6579-53de-467a-82bc-598fa1220819/IGVFFI8074JBCF.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep4_BinA-PCRrep11_S155_I2_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS6299IJHG/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:08:39.221643+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM4893GPVV/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM4893GPVV",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep4-BinA"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "released",
            "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T09:52:29.009771+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "9294f0be-c60c-45ef-b177-3b9616138751"
}