IGVFDS0548YILY

released

File Set Type

experimental data

Summary

mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Assay Term

in vitro CRISPR screen using flow cytometry

Targeted Genes

KITLG

Sample Summaries

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Construct Library Sets

IGVFDS7846UISG guide (sgRNA) library targeting accessible genome regions in many genomic loci

Protocols

https://www.protocols.io/view/crispri-of-regulatory-elements-upon-degron-operati-5jyl82d59l2w/v1

Aliases

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR1_S65

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR2_S71

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR3_S161

jesse-engreitz:measurement_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR4_S167

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4441KRKU

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item

Accession

Aliases

Sex

Taxa

IGVFDO0384OZRW

igvf:donor_of_HCT116

male

Homo sapiens

Auxiliary Datasets

2 items

Files

IGVFDS1005OGXR

AuxiliarySet

gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

IGVFFI2496VSIB, IGVFFI8901VLCQ, IGVFFI9347INON, IGVFFI3706KVDE

jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR1_S65
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR2_S71
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR3_S161
jesse-engreitz:auxiliary_CRUDO_KITLG-Auxin6hrs-FF2-BinE-PCR4_S167

Jesse Engreitz, Stanford

IGVFDS8781ZUPM

AuxiliarySet

cell sorting for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci, ... and 4 more measurement sets

IGVFFI9594ERWN