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IGVFDS0508ERUQ

released
File Set Type
experimental data
Summary
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
  • K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library
Protocols
Aliases
jay-shendure:CRISPRa_singlecell_K562_VPR_10Xlane2_exprs
Publications

Sequencing Results (Illumina)

6 items
Read Names
fastq
reads
R2
1
AAAHG2HHV
2
6.6 GB
Jay Shendure, UW
validated
fastq
reads
R2
1
AAAHG2HHV
1
6.7 GB
Jay Shendure, UW
validated
fastq
reads
R1
1
AAAHG2HHV
2
3.1 GB
Jay Shendure, UW
validated
fastq
reads
R1
1
AAAHG2HHV
1
3.1 GB
Jay Shendure, UW
validated
fastq
reads
I1
1
AAAHG2HHV
1
906 MB
Jay Shendure, UW
validated
fastq
reads
I1
1
AAAHG2HHV
2
898 MB
Jay Shendure, UW
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_K562
female
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
scCRISPR activation screen: differential gene expression quantifications, filtered feature barcode matrix
  • jay-shendure:analysis_set_CRISPRa_K562
Jay Shendure, UW

Auxiliary Datasets

1 item
Accession
Type
Summary
Files
Aliases
Lab
AuxiliarySet
gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
  • jay-shendure:CRISPRa_singlecell_K562_VPR_10Xlane2_CS1
Jay Shendure, UW

Attribution

Award
HG011966
Principal Investigator(s)
Contact P.I.
Jay Shendure
Lab
Jay Shendure, UW