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INDEXED
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MeasurementSet
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IGVFDS0508ERUQ
IGVFDS0508ERUQ
released
Json view
File Set Type
experimental data
Summary
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with a guide library
Construct Library Sets
IGVFDS7581DNXF
Guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
Protocols
https://www.protocols.io/view/methods-from-multiplex-single-cell-crispra-screeni-c5zvy766
Aliases
jay-shendure:CRISPRa_singlecell_K562_VPR_10Xlane2_exprs
Publications
Multiplex, single-cell CRISPRa screening for cell type specific regulatory elements
Sequencing Results (Illumina)
6 items
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI0662BBNP
fastq
reads
R2
1
Illumina NextSeq 2000
AAAHG2HHV
2
6.6 GB
Jay Shendure, UW
validated
IGVFFI1594NIYR
fastq
reads
R2
1
Illumina NextSeq 2000
AAAHG2HHV
1
6.7 GB
Jay Shendure, UW
validated
IGVFFI2492BPYV
fastq
reads
R1
1
Illumina NextSeq 2000
AAAHG2HHV
2
3.1 GB
Jay Shendure, UW
validated
IGVFFI2976CARS
fastq
reads
R1
1
Illumina NextSeq 2000
AAAHG2HHV
1
3.1 GB
Jay Shendure, UW
validated
IGVFFI5956RRFZ
fastq
reads
I1
1
Illumina NextSeq 2000
AAAHG2HHV
1
906 MB
Jay Shendure, UW
validated
IGVFFI8600XJSP
fastq
reads
I1
1
Illumina NextSeq 2000
AAAHG2HHV
2
898 MB
Jay Shendure, UW
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM9542NUGS
InVitroSystem
K562
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with a guide library
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO9208RPQQ
igvf:donor_of_K562
female
Homo sapiens
File Sets Using This Measurement Set as an Input
1 item
Accession
Type
Summary
Aliases
Lab
IGVFDS9078ZWQH
AnalysisSet
principal analysis of scCRISPR screen data
jay-shendure:analysis_set_CRISPRa_K562
Jay Shendure, UW
Auxiliary Datasets
1 item
Accession
Type
Summary
Files
Aliases
Lab
IGVFDS4834SKZS
AuxiliarySet
gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
IGVFFI6797WWMA
,
IGVFFI4295KZWO
,
IGVFFI3777QEJF
,
IGVFFI1773NVFV
,
IGVFFI2340WJKO
,
IGVFFI2735DIDC
jay-shendure:CRISPRa_singlecell_K562_VPR_10Xlane2_CS1
Jay Shendure, UW
Attribution
Award
HG011966
Principal Investigator(s)
Jay Shendure
,
Nadav Ahituv
,
Martin Kircher
Contact P.I.
Jay Shendure
Lab
Jay Shendure, UW