{
"@context": "/terms/",
"@id": "/measurement-sets/IGVFDS0410OHWU/",
"@type": [
"MeasurementSet",
"FileSet",
"Item"
],
"accession": "IGVFDS0410OHWU",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep3-BinC_AmpliconSequencing"
],
"assay_term": {
"@id": "/assay-terms/OBI_0003661/",
"assay_slims": [
"CRISPR screens"
],
"status": "released",
"term_name": "in vitro CRISPR screen using flow cytometry"
},
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released in vitro system [IGVFSM1041JPLJ](/in-vitro-systems/IGVFSM1041JPLJ/) has in progress subobject construct library set [IGVFDS6377KNCH](/construct-library-sets/IGVFDS6377KNCH/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/in-vitro-systems/IGVFSM1041JPLJ/"
},
{
"category": "missing publication",
"detail": "In vitro system [IGVFSM1041JPLJ](/in-vitro-systems/IGVFSM1041JPLJ/) has no `publications`. Released and archived samples are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_sample_missing_publication",
"path": "/in-vitro-systems/IGVFSM1041JPLJ/"
},
{
"category": "missing publication",
"detail": "Measurement set [IGVFDS0410OHWU](/measurement-sets/IGVFDS0410OHWU/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/measurement-sets/IGVFDS0410OHWU/"
},
{
"category": "missing sequencing kit",
"detail": "Measurement set [IGVFDS0410OHWU](/measurement-sets/IGVFDS0410OHWU/) has sequence file(s) [IGVFFI7265PKAK](/sequence-files/IGVFFI7265PKAK/), [IGVFFI6622EKZI](/sequence-files/IGVFFI6622EKZI/), [IGVFFI8593MYJO](/sequence-files/IGVFFI8593MYJO/), [IGVFFI3490PUBV](/sequence-files/IGVFFI3490PUBV/), [IGVFFI8253BOBU](/sequence-files/IGVFFI8253BOBU/), [IGVFFI1840YCSE](/sequence-files/IGVFFI1840YCSE/), [IGVFFI7179SMLX](/sequence-files/IGVFFI7179SMLX/), [IGVFFI5271OGTA](/sequence-files/IGVFFI5271OGTA/) which lack specification of a `sequencing_kit`. Sequence files in a file set associated wtih bulk data should specify a sequencing kit.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_inconsistent_sequencing_kit",
"path": "/measurement-sets/IGVFDS0410OHWU/"
},
{
"category": "missing sequence specification",
"detail": "Measurement set [IGVFDS0410OHWU](/measurement-sets/IGVFDS0410OHWU/) has sequence file(s): [IGVFFI7265PKAK](/sequence-files/IGVFFI7265PKAK/), [IGVFFI6622EKZI](/sequence-files/IGVFFI6622EKZI/), [IGVFFI8593MYJO](/sequence-files/IGVFFI8593MYJO/), [IGVFFI3490PUBV](/sequence-files/IGVFFI3490PUBV/), [IGVFFI8253BOBU](/sequence-files/IGVFFI8253BOBU/), [IGVFFI1840YCSE](/sequence-files/IGVFFI1840YCSE/), [IGVFFI7179SMLX](/sequence-files/IGVFFI7179SMLX/), [IGVFFI5271OGTA](/sequence-files/IGVFFI5271OGTA/) which do not have any `seqspecs`. Sequence files in a file set associated with bulk data are expected to link to a sequence specification file.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_missing_seqspec",
"path": "/measurement-sets/IGVFDS0410OHWU/"
}
],
"WARNING": [
{
"category": "missing auxiliary set",
"detail": "Measurement set [IGVFDS0410OHWU](/measurement-sets/IGVFDS0410OHWU/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_missing_auxiliary_set",
"path": "/measurement-sets/IGVFDS0410OHWU/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization",
"contact_pi": {
"@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
"title": "Jesse Engreitz"
},
"title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
},
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS6377KNCH/",
"accession": "IGVFDS6377KNCH",
"file_set_type": "guide library",
"status": "in progress",
"summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
}
],
"control_file_sets": [
{
"@id": "/measurement-sets/IGVFDS6385JXFZ/",
"accession": "IGVFDS6385JXFZ",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-THP1-PE2_Exp3_NegControl_AmpliconSequencing"
],
"status": "released"
}
],
"control_for": [],
"creation_timestamp": "2023-11-30T22:46:56.561758+00:00",
"donors": [
{
"@id": "/human-donors/IGVFDO7359LVPI/",
"accession": "IGVFDO7359LVPI",
"aliases": [
"igvf:donor_of_THP1"
],
"sex": "male",
"status": "released",
"taxa": "Homo sapiens"
}
],
"externally_hosted": false,
"file_set_type": "experimental data",
"files": [
{
"@id": "/sequence-files/IGVFFI7265PKAK/",
"accession": "IGVFFI7265PKAK",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep3-BinC-PCRrep2_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T18:53:33.669465+00:00",
"file_format": "fastq",
"file_size": 17594088,
"href": "/sequence-files/IGVFFI7265PKAK/@@download/IGVFFI7265PKAK.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/a761147e-b0f8-4f40-9bea-4a86565326f5/IGVFFI7265PKAK.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep2-FFRep3-BinC-PCRrep2_S134_R2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI6622EKZI/",
"accession": "IGVFFI6622EKZI",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep3-BinC-PCRrep1_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T18:34:20.336797+00:00",
"file_format": "fastq",
"file_size": 8446205,
"href": "/sequence-files/IGVFFI6622EKZI/@@download/IGVFFI6622EKZI.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/9252f1b9-f1d1-4bc2-84b8-34cb2d9d2f70/IGVFFI6622EKZI.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep2-FFRep3-BinC-PCRrep1_S38_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8593MYJO/",
"accession": "IGVFFI8593MYJO",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep3-BinC-PCRrep1_I1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T18:59:51.357533+00:00",
"file_format": "fastq",
"file_size": 2864872,
"href": "/sequence-files/IGVFFI8593MYJO/@@download/IGVFFI8593MYJO.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/9783b3ee-9c97-4e8e-9533-ca547f3a5f92/IGVFFI8593MYJO.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep2-FFRep3-BinC-PCRrep1_S38_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI3490PUBV/",
"accession": "IGVFFI3490PUBV",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep3-BinC-PCRrep1_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:12:13.152470+00:00",
"file_format": "fastq",
"file_size": 2780752,
"href": "/sequence-files/IGVFFI3490PUBV/@@download/IGVFFI3490PUBV.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/558e7404-88c3-4a5a-969b-a9fd16aa4bd7/IGVFFI3490PUBV.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep2-FFRep3-BinC-PCRrep1_S38_I2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI8253BOBU/",
"accession": "IGVFFI8253BOBU",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep3-BinC-PCRrep2_I1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:06:03.523053+00:00",
"file_format": "fastq",
"file_size": 2943917,
"href": "/sequence-files/IGVFFI8253BOBU/@@download/IGVFFI8253BOBU.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/5093d0f4-f76b-4c4d-bf79-90ff358f8d96/IGVFFI8253BOBU.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep2-FFRep3-BinC-PCRrep2_S134_I1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI1840YCSE/",
"accession": "IGVFFI1840YCSE",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep3-BinC-PCRrep1_R2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T18:47:02.167739+00:00",
"file_format": "fastq",
"file_size": 16525144,
"href": "/sequence-files/IGVFFI1840YCSE/@@download/IGVFFI1840YCSE.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/14ea0c21-59fe-4e0d-be01-8c55bd44b582/IGVFFI1840YCSE.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep2-FFRep3-BinC-PCRrep1_S38_R2_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI7179SMLX/",
"accession": "IGVFFI7179SMLX",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep3-BinC-PCRrep2_R1"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T18:40:37.566852+00:00",
"file_format": "fastq",
"file_size": 8558461,
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"s3_uri": "s3://igvf-files/2023/12/05/e6ba5b43-23f2-4ad1-9d5e-64cf23ebad27/IGVFFI7179SMLX.fastq.gz",
"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep2-FFRep3-BinC-PCRrep2_S134_R1_001.fastq.gz",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI5271OGTA/",
"accession": "IGVFFI5271OGTA",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep3-BinC-PCRrep2_I2"
],
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T19:18:23.558425+00:00",
"file_format": "fastq",
"file_size": 2874607,
"href": "/sequence-files/IGVFFI5271OGTA/@@download/IGVFFI5271OGTA.fastq.gz",
"s3_uri": "s3://igvf-files/2023/12/05/a719b471-3af8-48b2-8d5f-3b528a2afa75/IGVFFI5271OGTA.fastq.gz",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"status": "released",
"submitted_file_name": "220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep2-FFRep3-BinC-PCRrep2_S134_I2_001.fastq.gz",
"upload_status": "validated"
}
],
"input_for": [
"/analysis-sets/IGVFDS5056OAGR/"
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
"preferred_assay_title": "Variant-EFFECTS",
"protocols": [
"https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
],
"release_timestamp": "2023-12-20T23:10:09.365340+00:00",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM1041JPLJ/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1041JPLJ",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep3-BinC"
],
"classifications": [
"cell line"
],
"institutional_certificates": [],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0001253/",
"@type": [
"SampleTerm",
"OntologyTerm",
"Item"
],
"aliases": [
"igvf:THP-1"
],
"status": "released",
"summary": "THP-1",
"term_name": "THP-1"
}
],
"status": "released",
"summary": "Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 35-45% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library",
"taxa": "Homo sapiens"
}
],
"schema_version": "26",
"status": "released",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_files_timestamp": "2023-12-05T18:34:20.336797+00:00",
"summary": "CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
"targeted_genes": [
{
"@id": "/genes/ENSG00000108179/",
"geneid": "ENSG00000108179",
"name": "peptidylprolyl isomerase F",
"status": "released",
"symbol": "PPIF",
"synonyms": [
"PPIase F",
"peptidylprolyl isomerase F",
"Cyp-D",
"peptidyl-prolyl cis-trans isomerase F, mitochondrial",
"PPIF",
"CyP-M",
"cyclophilin F",
"peptidyl-prolyl cis-trans isomerase, mitochondrial",
"cyclophilin 3",
"cyclophilin D",
"rotamase F",
"mitochondrial cyclophilin",
"CypD",
"CYP3"
]
}
],
"uuid": "dd1fabe5-60b9-4413-b226-591ab0034a41"
}