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IGVFDS0385ESBQ

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File Set Type
experimental data
Summary
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
  • TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Construct Library Sets
  • IGVFDS1298TUYG guide (sgRNA) library targeting genes in many genomic loci
Protocols
Aliases
jesse-engreitz:measurement_set-scRNAseq_2kG_2PMDox1_6

Sequencing Results (Illumina)

3 items
fastq
reads
R1
1
11 GB
Jesse Engreitz, Stanford
validated
fastq
reads
R2
1
14 GB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
3.0 GB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_teloHAEC
female
Homo sapiens

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
interference Perturb-seq
  • jesse-engreitz:analysis_set_TeloHAEC_Perturb-seq_2kG
Jesse Engreitz, Stanford

Auxiliary Datasets

1 item
Accession
Type
Summary
Files
Aliases
Lab
AuxiliarySet
gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci
  • jesse-engreitz:auxiliary_sets-scRNAseq_2kG_2PMDox1_6
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford