IGVFDS0333TAKU

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS0333TAKU/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS0333TAKU",
    "aliases": [
        "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep4-BinA_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM7887QAEK](/in-vitro-systems/IGVFSM7887QAEK/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM7887QAEK/"
            },
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS0333TAKU](/measurement-sets/IGVFDS0333TAKU/) has sequence file(s) [IGVFFI4575KAIW](/sequence-files/IGVFFI4575KAIW/), [IGVFFI3707PDQM](/sequence-files/IGVFFI3707PDQM/), [IGVFFI1462PDEY](/sequence-files/IGVFFI1462PDEY/), [IGVFFI8000AXTW](/sequence-files/IGVFFI8000AXTW/), [IGVFFI5701UGPX](/sequence-files/IGVFFI5701UGPX/), [IGVFFI5051HZOM](/sequence-files/IGVFFI5051HZOM/), [IGVFFI1431VMMF](/sequence-files/IGVFFI1431VMMF/), [IGVFFI1426BWAB](/sequence-files/IGVFFI1426BWAB/), [IGVFFI0174USAR](/sequence-files/IGVFFI0174USAR/), [IGVFFI6204GUIT](/sequence-files/IGVFFI6204GUIT/), [IGVFFI4569XYJP](/sequence-files/IGVFFI4569XYJP/), [IGVFFI5274TVBZ](/sequence-files/IGVFFI5274TVBZ/), [IGVFFI6991FPKD](/sequence-files/IGVFFI6991FPKD/), [IGVFFI1064UYZV](/sequence-files/IGVFFI1064UYZV/), [IGVFFI6196BAOW](/sequence-files/IGVFFI6196BAOW/), [IGVFFI2848WEPJ](/sequence-files/IGVFFI2848WEPJ/), [IGVFFI8297FNWK](/sequence-files/IGVFFI8297FNWK/), [IGVFFI6720ZEEX](/sequence-files/IGVFFI6720ZEEX/), [IGVFFI8793NYKD](/sequence-files/IGVFFI8793NYKD/), [IGVFFI9654RPED](/sequence-files/IGVFFI9654RPED/), [IGVFFI3476MBUB](/sequence-files/IGVFFI3476MBUB/), [IGVFFI8627SPVH](/sequence-files/IGVFFI8627SPVH/), [IGVFFI3261KWLF](/sequence-files/IGVFFI3261KWLF/), [IGVFFI1845WBIW](/sequence-files/IGVFFI1845WBIW/), [IGVFFI9831JAXI](/sequence-files/IGVFFI9831JAXI/), [IGVFFI9036LSPR](/sequence-files/IGVFFI9036LSPR/), [IGVFFI8707LFXT](/sequence-files/IGVFFI8707LFXT/), [IGVFFI5662SNOZ](/sequence-files/IGVFFI5662SNOZ/), [IGVFFI2646YWCX](/sequence-files/IGVFFI2646YWCX/), [IGVFFI8822TWFI](/sequence-files/IGVFFI8822TWFI/), [IGVFFI4918WGKK](/sequence-files/IGVFFI4918WGKK/), [IGVFFI4920SLPO](/sequence-files/IGVFFI4920SLPO/), [IGVFFI5731OQND](/sequence-files/IGVFFI5731OQND/), [IGVFFI2921IEOK](/sequence-files/IGVFFI2921IEOK/), [IGVFFI6766RHAF](/sequence-files/IGVFFI6766RHAF/), [IGVFFI4367KBDD](/sequence-files/IGVFFI4367KBDD/), [IGVFFI4920UPLF](/sequence-files/IGVFFI4920UPLF/), [IGVFFI0222FTDC](/sequence-files/IGVFFI0222FTDC/), [IGVFFI8249RMDX](/sequence-files/IGVFFI8249RMDX/), [IGVFFI7099BDRG](/sequence-files/IGVFFI7099BDRG/), [IGVFFI3774EFGR](/sequence-files/IGVFFI3774EFGR/), [IGVFFI6945HKHO](/sequence-files/IGVFFI6945HKHO/), [IGVFFI5007IUMS](/sequence-files/IGVFFI5007IUMS/), [IGVFFI4398XITN](/sequence-files/IGVFFI4398XITN/), [IGVFFI0352WGND](/sequence-files/IGVFFI0352WGND/), [IGVFFI1562GJLN](/sequence-files/IGVFFI1562GJLN/), [IGVFFI5316SRFE](/sequence-files/IGVFFI5316SRFE/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS0333TAKU/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS0333TAKU](/measurement-sets/IGVFDS0333TAKU/) has sequence file(s): [IGVFFI4575KAIW](/sequence-files/IGVFFI4575KAIW/), [IGVFFI3707PDQM](/sequence-files/IGVFFI3707PDQM/), [IGVFFI1462PDEY](/sequence-files/IGVFFI1462PDEY/), [IGVFFI8000AXTW](/sequence-files/IGVFFI8000AXTW/), [IGVFFI5701UGPX](/sequence-files/IGVFFI5701UGPX/), [IGVFFI5051HZOM](/sequence-files/IGVFFI5051HZOM/), [IGVFFI1431VMMF](/sequence-files/IGVFFI1431VMMF/), [IGVFFI1426BWAB](/sequence-files/IGVFFI1426BWAB/), [IGVFFI0174USAR](/sequence-files/IGVFFI0174USAR/), [IGVFFI6204GUIT](/sequence-files/IGVFFI6204GUIT/), [IGVFFI4569XYJP](/sequence-files/IGVFFI4569XYJP/), [IGVFFI5274TVBZ](/sequence-files/IGVFFI5274TVBZ/), [IGVFFI6991FPKD](/sequence-files/IGVFFI6991FPKD/), [IGVFFI1064UYZV](/sequence-files/IGVFFI1064UYZV/), [IGVFFI6196BAOW](/sequence-files/IGVFFI6196BAOW/), [IGVFFI2848WEPJ](/sequence-files/IGVFFI2848WEPJ/), [IGVFFI8297FNWK](/sequence-files/IGVFFI8297FNWK/), [IGVFFI6720ZEEX](/sequence-files/IGVFFI6720ZEEX/), [IGVFFI8793NYKD](/sequence-files/IGVFFI8793NYKD/), [IGVFFI9654RPED](/sequence-files/IGVFFI9654RPED/), [IGVFFI3476MBUB](/sequence-files/IGVFFI3476MBUB/), [IGVFFI8627SPVH](/sequence-files/IGVFFI8627SPVH/), [IGVFFI3261KWLF](/sequence-files/IGVFFI3261KWLF/), [IGVFFI1845WBIW](/sequence-files/IGVFFI1845WBIW/), [IGVFFI9831JAXI](/sequence-files/IGVFFI9831JAXI/), [IGVFFI9036LSPR](/sequence-files/IGVFFI9036LSPR/), [IGVFFI8707LFXT](/sequence-files/IGVFFI8707LFXT/), [IGVFFI5662SNOZ](/sequence-files/IGVFFI5662SNOZ/), [IGVFFI2646YWCX](/sequence-files/IGVFFI2646YWCX/), [IGVFFI8822TWFI](/sequence-files/IGVFFI8822TWFI/), [IGVFFI4918WGKK](/sequence-files/IGVFFI4918WGKK/), [IGVFFI4920SLPO](/sequence-files/IGVFFI4920SLPO/), [IGVFFI5731OQND](/sequence-files/IGVFFI5731OQND/), [IGVFFI2921IEOK](/sequence-files/IGVFFI2921IEOK/), [IGVFFI6766RHAF](/sequence-files/IGVFFI6766RHAF/), [IGVFFI4367KBDD](/sequence-files/IGVFFI4367KBDD/), [IGVFFI4920UPLF](/sequence-files/IGVFFI4920UPLF/), [IGVFFI0222FTDC](/sequence-files/IGVFFI0222FTDC/), [IGVFFI8249RMDX](/sequence-files/IGVFFI8249RMDX/), [IGVFFI7099BDRG](/sequence-files/IGVFFI7099BDRG/), [IGVFFI3774EFGR](/sequence-files/IGVFFI3774EFGR/), [IGVFFI6945HKHO](/sequence-files/IGVFFI6945HKHO/), [IGVFFI5007IUMS](/sequence-files/IGVFFI5007IUMS/), [IGVFFI4398XITN](/sequence-files/IGVFFI4398XITN/), [IGVFFI0352WGND](/sequence-files/IGVFFI0352WGND/), [IGVFFI1562GJLN](/sequence-files/IGVFFI1562GJLN/), [IGVFFI5316SRFE](/sequence-files/IGVFFI5316SRFE/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS0333TAKU/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS0333TAKU](/measurement-sets/IGVFDS0333TAKU/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS0333TAKU/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
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            "@id": "/measurement-sets/IGVFDS4869FXYY/",
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            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:48:14.810527+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep4-BinA"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "released",
            "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T10:24:16.058647+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "f3a508a6-9a40-40e7-8808-1b68515c8341"
}