IGVFDS0329UHSG

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS0329UHSG/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS0329UHSG",
    "aliases": [
        "jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep1-BinA_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "assay_slims": [
            "CRISPR screens"
        ],
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing publication",
                "detail": "Measurement set [IGVFDS0329UHSG](/measurement-sets/IGVFDS0329UHSG/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_file_set_missing_publication",
                "path": "/measurement-sets/IGVFDS0329UHSG/"
            },
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS0329UHSG](/measurement-sets/IGVFDS0329UHSG/) has sequence file(s) [IGVFFI8058IJZH](/sequence-files/IGVFFI8058IJZH/), [IGVFFI1689MKOK](/sequence-files/IGVFFI1689MKOK/), [IGVFFI2015TTTJ](/sequence-files/IGVFFI2015TTTJ/), [IGVFFI6068UEFK](/sequence-files/IGVFFI6068UEFK/), [IGVFFI6632LIMD](/sequence-files/IGVFFI6632LIMD/), [IGVFFI7530SUEE](/sequence-files/IGVFFI7530SUEE/), [IGVFFI1869QKXY](/sequence-files/IGVFFI1869QKXY/), [IGVFFI6252FOFU](/sequence-files/IGVFFI6252FOFU/) which lack specification of a `sequencing_kit`. Sequence files in a file set associated wtih bulk data should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS0329UHSG/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS0329UHSG](/measurement-sets/IGVFDS0329UHSG/) has sequence file(s): [IGVFFI8058IJZH](/sequence-files/IGVFFI8058IJZH/), [IGVFFI1689MKOK](/sequence-files/IGVFFI1689MKOK/), [IGVFFI2015TTTJ](/sequence-files/IGVFFI2015TTTJ/), [IGVFFI6068UEFK](/sequence-files/IGVFFI6068UEFK/), [IGVFFI6632LIMD](/sequence-files/IGVFFI6632LIMD/), [IGVFFI7530SUEE](/sequence-files/IGVFFI7530SUEE/), [IGVFFI1869QKXY](/sequence-files/IGVFFI1869QKXY/), [IGVFFI6252FOFU](/sequence-files/IGVFFI6252FOFU/) which do not have any `seqspecs`. Sequence files in a file set associated with bulk data are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS0329UHSG/"
            },
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM9398KLPK](/in-vitro-systems/IGVFSM9398KLPK/) has in progress subobject construct library set [IGVFDS6377KNCH](/construct-library-sets/IGVFDS6377KNCH/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM9398KLPK/"
            },
            {
                "category": "missing publication",
                "detail": "In vitro system [IGVFSM9398KLPK](/in-vitro-systems/IGVFSM9398KLPK/) has no `publications`. Released and archived samples are expected to be associated with a publication.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_sample_missing_publication",
                "path": "/in-vitro-systems/IGVFSM9398KLPK/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS0329UHSG](/measurement-sets/IGVFDS0329UHSG/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS0329UHSG/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
    "construct_library_sets": [
        {
            "@id": "/construct-library-sets/IGVFDS6377KNCH/",
            "accession": "IGVFDS6377KNCH",
            "file_set_type": "guide library",
            "status": "in progress",
            "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
        }
    ],
    "control_file_sets": [
        {
            "@id": "/measurement-sets/IGVFDS9477ARWI/",
            "accession": "IGVFDS9477ARWI",
            "aliases": [
                "jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing",
                "jesse-engreitz:220829_8merInsertion-Jurkat_Stimulation-Amp_PPIF_promoter-Jurkat-PE2_Exp1_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-11-30T22:47:30.261281+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
    "files": [
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            "@id": "/sequence-files/IGVFFI8058IJZH/",
            "accession": "IGVFFI8058IJZH",
            "aliases": [
                "jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep1-BinA-PCRrep2_R1"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2023-12-05T18:50:18.096467+00:00",
            "file_format": "fastq",
            "file_size": 7761331,
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            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF_S7-BioRep3-FFRep1-BinA-PCRrep2_S85_R1_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI1689MKOK/",
            "accession": "IGVFFI1689MKOK",
            "aliases": [
                "jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep1-BinA-PCRrep2_I1"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2023-12-05T19:15:58.018277+00:00",
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            "sequencing_platform": {
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                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF_S7-BioRep3-FFRep1-BinA-PCRrep2_S85_I1_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI2015TTTJ/",
            "accession": "IGVFFI2015TTTJ",
            "aliases": [
                "jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep1-BinA-PCRrep1_R1"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2023-12-05T18:43:59.204136+00:00",
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            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF_S7-BioRep3-FFRep1-BinA-PCRrep1_S29_R1_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI6068UEFK/",
            "accession": "IGVFFI6068UEFK",
            "aliases": [
                "jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep1-BinA-PCRrep1_I2"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2023-12-05T19:22:14.662491+00:00",
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            "sequencing_platform": {
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                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF_S7-BioRep3-FFRep1-BinA-PCRrep1_S29_I2_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI6632LIMD/",
            "accession": "IGVFFI6632LIMD",
            "aliases": [
                "jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep1-BinA-PCRrep2_I2"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2023-12-05T19:28:33.654314+00:00",
            "file_format": "fastq",
            "file_size": 3151947,
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            "sequencing_platform": {
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                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF_S7-BioRep3-FFRep1-BinA-PCRrep2_S85_I2_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI7530SUEE/",
            "accession": "IGVFFI7530SUEE",
            "aliases": [
                "jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep1-BinA-PCRrep2_R2"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2023-12-05T19:03:22.888474+00:00",
            "file_format": "fastq",
            "file_size": 14083616,
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            "sequencing_platform": {
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            },
            "status": "released",
            "submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF_S7-BioRep3-FFRep1-BinA-PCRrep2_S85_R2_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI1869QKXY/",
            "accession": "IGVFFI1869QKXY",
            "aliases": [
                "jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep1-BinA-PCRrep1_I1"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2023-12-05T19:09:42.548668+00:00",
            "file_format": "fastq",
            "file_size": 3231751,
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            "sequencing_platform": {
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            },
            "status": "released",
            "submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF_S7-BioRep3-FFRep1-BinA-PCRrep1_S29_I1_001.fastq.gz",
            "upload_status": "validated"
        },
        {
            "@id": "/sequence-files/IGVFFI6252FOFU/",
            "accession": "IGVFFI6252FOFU",
            "aliases": [
                "jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep1-BinA-PCRrep1_R2"
            ],
            "content_type": "reads",
            "controlled_access": false,
            "creation_timestamp": "2023-12-05T18:56:45.900881+00:00",
            "file_format": "fastq",
            "file_size": 12921148,
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            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "220829_pPPIF_JurkatVFF-Jurkat-PE2A-BFP-pPPIF-Amp_pPPIF_S7-BioRep3-FFRep1-BinA-PCRrep1_S29_R2_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS9162RHFP/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:04:40.853030+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM9398KLPK/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM9398KLPK",
            "aliases": [
                "jesse-engreitz:220829_8merInsertion-Jurkat_NoStimulation-Amp_PPIF_promoter-BioRep3-FFrep1-BinA"
            ],
            "classifications": [
                "cell line"
            ],
            "institutional_certificates": [],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "released",
            "summary": "Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 0-10% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "26",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-05T18:43:59.204136+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "PPIase F",
                "peptidylprolyl isomerase F",
                "Cyp-D",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIF",
                "CyP-M",
                "cyclophilin F",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "cyclophilin 3",
                "cyclophilin D",
                "rotamase F",
                "mitochondrial cyclophilin",
                "CypD",
                "CYP3"
            ]
        }
    ],
    "uuid": "44dc3d34-c896-4171-ba4f-c1a273787cf1"
}