IGVFDS0238VKNF

released

File Set Type

experimental data

Summary

activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes

Assay Term

in vitro CRISPR screen using single-cell RNA-seq

Sample Summaries

CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Construct Library Sets

IGVFDS0821YMMV Guide (sgRNA) library targeting transcription start sites in 24 genes

Protocols

Aliases

charles-gersbach:cd8-tcells-perturb-seq-crispra-gex-rep3

Publications

Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens

Sequencing Results (Illumina)

8 items

IGVFFI4462ZXLC

fastq

reads

IGVFFI6012DHNO

Illumina NovaSeq 6000

4.6 GB

Charles Gersbach, Duke

validated

IGVFFI6398QCVG

fastq

reads

IGVFFI0994QEPT

Illumina NovaSeq 6000

4.6 GB

Charles Gersbach, Duke

validated

IGVFFI6606ARBS

fastq

reads

IGVFFI0994QEPT

Illumina NovaSeq 6000

11 GB

Charles Gersbach, Duke

validated

IGVFFI6761ZNFE

fastq

reads

IGVFFI8536YCHC

Illumina NovaSeq 6000

4.6 GB

Charles Gersbach, Duke

validated

IGVFFI6787BWWZ

fastq

reads

IGVFFI8536YCHC

Illumina NovaSeq 6000

11 GB

Charles Gersbach, Duke

validated

IGVFFI7249VOBK

fastq

reads

IGVFFI5760CKQR

Illumina NovaSeq 6000

11 GB

Charles Gersbach, Duke

validated

IGVFFI7868FNQC

fastq

reads

IGVFFI5760CKQR

Illumina NovaSeq 6000

4.6 GB

Charles Gersbach, Duke

validated

IGVFFI8501DMZJ

fastq

reads

IGVFFI6012DHNO

Illumina NovaSeq 6000

11 GB

Charles Gersbach, Duke

validated

Attribution

Award

HG012053

Principal Investigator(s)

Charles Gersbach, Gregory Crawford, Tim Reddy

Contact P.I.

Charles Gersbach

Lab

Charles Gersbach, Duke

IGVFDS0238VKNF

Sequencing Results (Illumina)

Samples

Donors

File Sets Using This Measurement Set as an Input

Auxiliary Datasets

Attribution