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IGVFDS0238VKNF

released
File Set Type
experimental data
Summary
CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
Assay Term
in vitro CRISPR screen using single-cell RNA-seq
Sample Summaries
  • CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Construct Library Sets
  • IGVFDS0821YMMV guide (sgRNA) library targeting transcription start sites in 24 genes
Protocols
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispra-gex-rep3
Publications

Sequencing Results (Illumina)

8 items
fastq
reads
R1
3
4
4.6 GB
Charles Gersbach, Duke
validated
fastq
reads
R1
3
3
4.6 GB
Charles Gersbach, Duke
validated
fastq
reads
R2
3
3
11 GB
Charles Gersbach, Duke
validated
fastq
reads
R1
3
1
4.6 GB
Charles Gersbach, Duke
validated
fastq
reads
R2
3
1
11 GB
Charles Gersbach, Duke
validated
fastq
reads
R2
3
2
11 GB
Charles Gersbach, Duke
validated
fastq
reads
R1
3
2
4.6 GB
Charles Gersbach, Duke
validated
fastq
reads
R2
3
4
11 GB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:cd8-tcells-donor-8
unspecified
Homo sapiens

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
activation Perturb-seq: filtered feature barcode matrix
  • charles-gersbach:cd8-tcells-intermediate-analysis-set-perturb-seq-crispra
Charles Gersbach, Duke

Auxiliary Datasets

1 item
Accession
Type
Summary
Files
Aliases
Lab
AuxiliarySet
gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
  • charles-gersbach:cd8-tcells-perturb-seq-crispra-grna-rep3
Charles Gersbach, Duke

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke