Home

IGVFDS0071OLHI

released
File Set Type
experimental data
Summary
CRISPR interference FlowFISH screen sorted on the expression of TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus
Assay Term
in vitro CRISPR screen using flow cytometry
Targeted Genes
Sample Summaries
  • TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin for 51-75% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
Protocols
Aliases
jesse-engreitz:measurement_set_eRZ10_teloHAEC_TLNRD1_enhancer_CRISPRi_FlowFISH_Rep1_bin3

Sequencing Results (Illumina)

1 item
Accession
File Format
Content Type
Illumina Read Type
Sequencing Run
Read Names
Associated seqspec Files
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
R1
1
67 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Associated Construct Library Sets
Summary
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin for 51-75% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Donors

1 item
Accession
Aliases
Sex
Taxa
  • igvf:donor_of_teloHAEC
female
Homo sapiens

Associated Construct Library Sets

1 item
Accession
Summary
Library Design Files
guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus

File Sets Using This Measurement Set as an Input

1 item
Accession
Type
Summary
Aliases
Lab
AnalysisSet
CRISPR interference FlowFISH screen targeting TLNRD1
  • jesse-engreitz:analysis_set_eRZ10_teloHAEC_TLNRD1_enhancer_CRISPRi_FlowFISH_analysis
Jesse Engreitz, Stanford

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford