IGVFDS0022UYQK

released
{
    "@context": "/terms/",
    "@id": "/measurement-sets/IGVFDS0022UYQK/",
    "@type": [
        "MeasurementSet",
        "FileSet",
        "Item"
    ],
    "accession": "IGVFDS0022UYQK",
    "aliases": [
        "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep3-BinB_AmpliconSequencing"
    ],
    "assay_term": {
        "@id": "/assay-terms/OBI_0003661/",
        "status": "released",
        "term_name": "in vitro CRISPR screen using flow cytometry"
    },
    "audit": {
        "INTERNAL_ACTION": [
            {
                "category": "missing sequencing kit",
                "detail": "Measurement set [IGVFDS0022UYQK](/measurement-sets/IGVFDS0022UYQK/) has sequence file(s) [IGVFFI1089XLDA](/sequence-files/IGVFFI1089XLDA/), [IGVFFI0691IQPZ](/sequence-files/IGVFFI0691IQPZ/), [IGVFFI9755SPNW](/sequence-files/IGVFFI9755SPNW/), [IGVFFI6614UOWJ](/sequence-files/IGVFFI6614UOWJ/), [IGVFFI4048MNSW](/sequence-files/IGVFFI4048MNSW/), [IGVFFI1420YJRZ](/sequence-files/IGVFFI1420YJRZ/), [IGVFFI7697UDFW](/sequence-files/IGVFFI7697UDFW/), [IGVFFI5432MJFD](/sequence-files/IGVFFI5432MJFD/), [IGVFFI2627NGVH](/sequence-files/IGVFFI2627NGVH/), [IGVFFI5304XHYN](/sequence-files/IGVFFI5304XHYN/), [IGVFFI7823CFZV](/sequence-files/IGVFFI7823CFZV/), [IGVFFI9091AGBC](/sequence-files/IGVFFI9091AGBC/), [IGVFFI8133DDNI](/sequence-files/IGVFFI8133DDNI/), [IGVFFI0053VRWL](/sequence-files/IGVFFI0053VRWL/), [IGVFFI1017UTUR](/sequence-files/IGVFFI1017UTUR/), [IGVFFI1611WXTW](/sequence-files/IGVFFI1611WXTW/), [IGVFFI5967UVLA](/sequence-files/IGVFFI5967UVLA/), [IGVFFI1649WGHW](/sequence-files/IGVFFI1649WGHW/), [IGVFFI4219FXDQ](/sequence-files/IGVFFI4219FXDQ/), [IGVFFI7107ANPB](/sequence-files/IGVFFI7107ANPB/), [IGVFFI6200KIOA](/sequence-files/IGVFFI6200KIOA/), [IGVFFI5120XPHJ](/sequence-files/IGVFFI5120XPHJ/), [IGVFFI4619WOCL](/sequence-files/IGVFFI4619WOCL/), [IGVFFI4252MKOG](/sequence-files/IGVFFI4252MKOG/), [IGVFFI1026SBLA](/sequence-files/IGVFFI1026SBLA/), [IGVFFI9037SEFD](/sequence-files/IGVFFI9037SEFD/), [IGVFFI9983YJQH](/sequence-files/IGVFFI9983YJQH/), [IGVFFI2222FCSX](/sequence-files/IGVFFI2222FCSX/), [IGVFFI1632XCGX](/sequence-files/IGVFFI1632XCGX/), [IGVFFI0905HGFV](/sequence-files/IGVFFI0905HGFV/), [IGVFFI1356PVVE](/sequence-files/IGVFFI1356PVVE/), [IGVFFI1450AZDX](/sequence-files/IGVFFI1450AZDX/), [IGVFFI0549QXSE](/sequence-files/IGVFFI0549QXSE/), [IGVFFI2288HNPG](/sequence-files/IGVFFI2288HNPG/), [IGVFFI7102ATDM](/sequence-files/IGVFFI7102ATDM/), [IGVFFI2693EXGY](/sequence-files/IGVFFI2693EXGY/), [IGVFFI9603YDUB](/sequence-files/IGVFFI9603YDUB/), [IGVFFI6523ULUC](/sequence-files/IGVFFI6523ULUC/), [IGVFFI8004NHDR](/sequence-files/IGVFFI8004NHDR/), [IGVFFI6892WDZV](/sequence-files/IGVFFI6892WDZV/), [IGVFFI2082SYOE](/sequence-files/IGVFFI2082SYOE/), [IGVFFI1074HOXI](/sequence-files/IGVFFI1074HOXI/), [IGVFFI5752SSZF](/sequence-files/IGVFFI5752SSZF/), [IGVFFI5436ZQLU](/sequence-files/IGVFFI5436ZQLU/), [IGVFFI0664MQGJ](/sequence-files/IGVFFI0664MQGJ/), [IGVFFI9288MPPA](/sequence-files/IGVFFI9288MPPA/), [IGVFFI5980OVLF](/sequence-files/IGVFFI5980OVLF/), [IGVFFI0915TPPR](/sequence-files/IGVFFI0915TPPR/) which lack specification of a `sequencing_kit`. Sequence files should specify a sequencing kit.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_inconsistent_sequencing_kit",
                "path": "/measurement-sets/IGVFDS0022UYQK/"
            },
            {
                "category": "missing sequence specification",
                "detail": "Measurement set [IGVFDS0022UYQK](/measurement-sets/IGVFDS0022UYQK/) has sequence file(s): [IGVFFI1089XLDA](/sequence-files/IGVFFI1089XLDA/), [IGVFFI0691IQPZ](/sequence-files/IGVFFI0691IQPZ/), [IGVFFI9755SPNW](/sequence-files/IGVFFI9755SPNW/), [IGVFFI6614UOWJ](/sequence-files/IGVFFI6614UOWJ/), [IGVFFI4048MNSW](/sequence-files/IGVFFI4048MNSW/), [IGVFFI1420YJRZ](/sequence-files/IGVFFI1420YJRZ/), [IGVFFI7697UDFW](/sequence-files/IGVFFI7697UDFW/), [IGVFFI5432MJFD](/sequence-files/IGVFFI5432MJFD/), [IGVFFI2627NGVH](/sequence-files/IGVFFI2627NGVH/), [IGVFFI5304XHYN](/sequence-files/IGVFFI5304XHYN/), [IGVFFI7823CFZV](/sequence-files/IGVFFI7823CFZV/), [IGVFFI9091AGBC](/sequence-files/IGVFFI9091AGBC/), [IGVFFI8133DDNI](/sequence-files/IGVFFI8133DDNI/), [IGVFFI0053VRWL](/sequence-files/IGVFFI0053VRWL/), [IGVFFI1017UTUR](/sequence-files/IGVFFI1017UTUR/), [IGVFFI1611WXTW](/sequence-files/IGVFFI1611WXTW/), [IGVFFI5967UVLA](/sequence-files/IGVFFI5967UVLA/), [IGVFFI1649WGHW](/sequence-files/IGVFFI1649WGHW/), [IGVFFI4219FXDQ](/sequence-files/IGVFFI4219FXDQ/), [IGVFFI7107ANPB](/sequence-files/IGVFFI7107ANPB/), [IGVFFI6200KIOA](/sequence-files/IGVFFI6200KIOA/), [IGVFFI5120XPHJ](/sequence-files/IGVFFI5120XPHJ/), [IGVFFI4619WOCL](/sequence-files/IGVFFI4619WOCL/), [IGVFFI4252MKOG](/sequence-files/IGVFFI4252MKOG/), [IGVFFI1026SBLA](/sequence-files/IGVFFI1026SBLA/), [IGVFFI9037SEFD](/sequence-files/IGVFFI9037SEFD/), [IGVFFI9983YJQH](/sequence-files/IGVFFI9983YJQH/), [IGVFFI2222FCSX](/sequence-files/IGVFFI2222FCSX/), [IGVFFI1632XCGX](/sequence-files/IGVFFI1632XCGX/), [IGVFFI0905HGFV](/sequence-files/IGVFFI0905HGFV/), [IGVFFI1356PVVE](/sequence-files/IGVFFI1356PVVE/), [IGVFFI1450AZDX](/sequence-files/IGVFFI1450AZDX/), [IGVFFI0549QXSE](/sequence-files/IGVFFI0549QXSE/), [IGVFFI2288HNPG](/sequence-files/IGVFFI2288HNPG/), [IGVFFI7102ATDM](/sequence-files/IGVFFI7102ATDM/), [IGVFFI2693EXGY](/sequence-files/IGVFFI2693EXGY/), [IGVFFI9603YDUB](/sequence-files/IGVFFI9603YDUB/), [IGVFFI6523ULUC](/sequence-files/IGVFFI6523ULUC/), [IGVFFI8004NHDR](/sequence-files/IGVFFI8004NHDR/), [IGVFFI6892WDZV](/sequence-files/IGVFFI6892WDZV/), [IGVFFI2082SYOE](/sequence-files/IGVFFI2082SYOE/), [IGVFFI1074HOXI](/sequence-files/IGVFFI1074HOXI/), [IGVFFI5752SSZF](/sequence-files/IGVFFI5752SSZF/), [IGVFFI5436ZQLU](/sequence-files/IGVFFI5436ZQLU/), [IGVFFI0664MQGJ](/sequence-files/IGVFFI0664MQGJ/), [IGVFFI9288MPPA](/sequence-files/IGVFFI9288MPPA/), [IGVFFI5980OVLF](/sequence-files/IGVFFI5980OVLF/), [IGVFFI0915TPPR](/sequence-files/IGVFFI0915TPPR/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_missing_seqspec",
                "path": "/measurement-sets/IGVFDS0022UYQK/"
            },
            {
                "category": "mismatched status",
                "detail": "Released in vitro system [IGVFSM9112SDPK](/in-vitro-systems/IGVFSM9112SDPK/) has in progress subobject construct library set [IGVFDS2385DHZO](/construct-library-sets/IGVFDS2385DHZO/).",
                "level": 30,
                "level_name": "INTERNAL_ACTION",
                "name": "audit_item_status",
                "path": "/in-vitro-systems/IGVFSM9112SDPK/"
            }
        ],
        "WARNING": [
            {
                "category": "missing auxiliary set",
                "detail": "Measurement set [IGVFDS0022UYQK](/measurement-sets/IGVFDS0022UYQK/) has no cell sorting `auxiliary_sets`. CRISPR-based measurement sets that utilize flow cytometry are expected to link to a cell sorting auxiliary set.",
                "level": 40,
                "level_name": "WARNING",
                "name": "audit_missing_auxiliary_set",
                "path": "/measurement-sets/IGVFDS0022UYQK/"
            }
        ]
    },
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization",
        "contact_pi": {
            "@id": "/users/6c75dbfa-635e-4a7a-8ec8-9e51e9c380a4/",
            "title": "Jesse Engreitz"
        },
        "title": "Stanford Center for Connecting DNA Variants to Function and Phenotype"
    },
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            "@id": "/measurement-sets/IGVFDS4869FXYY/",
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                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-Jurkat-PE2_Exp2_NegControl_AmpliconSequencing"
            ],
            "status": "released"
        }
    ],
    "control_for": [],
    "creation_timestamp": "2023-12-13T08:48:13.523199+00:00",
    "donors": [
        {
            "@id": "/human-donors/IGVFDO1080XFGV/",
            "accession": "IGVFDO1080XFGV",
            "aliases": [
                "igvf:donor_of_Jurkat"
            ],
            "sex": "male",
            "status": "released",
            "taxa": "Homo sapiens"
        }
    ],
    "externally_hosted": false,
    "file_set_type": "experimental data",
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            "s3_uri": "s3://igvf-files/2023/12/13/877466c0-0fc9-48a4-9879-5e97243d1963/IGVFFI0915TPPR.fastq.gz",
            "sequencing_platform": {
                "@id": "/platform-terms/EFO_0008566/",
                "status": "released",
                "term_name": "Illumina NextSeq 550"
            },
            "status": "released",
            "submitted_file_name": "230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep2-FFRep3_BinB-PCRrep4_S304_I2_001.fastq.gz",
            "upload_status": "validated"
        }
    ],
    "input_for": [
        "/analysis-sets/IGVFDS6299IJHG/"
    ],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "notes": "The readout 2e41e624-f6c7-4f0c-a07e-bc36cefdca39 was removed from this measurement set. This measurement set previously used Variant FlowFISH as a preferred_assay_title, but this enum is now removed. So it has been defaulted to Variant-EFFECTS.",
    "preferred_assay_title": "Variant-EFFECTS",
    "protocols": [
        "https://www.protocols.io/view/flowfish-with-primeflow-36wgq3q4olk5/v1"
    ],
    "release_timestamp": "2023-12-20T23:08:36.278760+00:00",
    "samples": [
        {
            "@id": "/in-vitro-systems/IGVFSM9112SDPK/",
            "@type": [
                "InVitroSystem",
                "Biosample",
                "Sample",
                "Item"
            ],
            "accession": "IGVFSM9112SDPK",
            "aliases": [
                "jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep2-FFrep3-BinB"
            ],
            "classifications": [
                "cell line"
            ],
            "construct_library_sets": [
                {
                    "@id": "/construct-library-sets/IGVFDS2385DHZO/",
                    "accession": "IGVFDS2385DHZO",
                    "file_set_type": "guide library",
                    "status": "in progress",
                    "summary": "guide (sgRNA) library targeting sequence variants in a genomic locus"
                }
            ],
            "modifications": [
                {
                    "@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
                    "modality": "prime editing",
                    "status": "released"
                }
            ],
            "sample_terms": [
                {
                    "@id": "/sample-terms/EFO_0002796/",
                    "@type": [
                        "SampleTerm",
                        "OntologyTerm",
                        "Item"
                    ],
                    "aliases": [
                        "igvf:Jurkat"
                    ],
                    "status": "released",
                    "summary": "Jurkat",
                    "term_name": "Jurkat"
                }
            ],
            "status": "released",
            "summary": "Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
            "taxa": "Homo sapiens"
        }
    ],
    "schema_version": "23",
    "status": "released",
    "submitted_by": {
        "@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
        "title": "Michael Montgomery"
    },
    "submitted_files_timestamp": "2023-12-13T10:16:32.277787+00:00",
    "summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus",
    "targeted_genes": [
        {
            "@id": "/genes/ENSG00000108179/",
            "geneid": "ENSG00000108179",
            "name": "peptidylprolyl isomerase F",
            "status": "released",
            "symbol": "PPIF",
            "synonyms": [
                "rotamase F",
                "CYP3",
                "cyclophilin F",
                "mitochondrial cyclophilin",
                "peptidyl-prolyl cis-trans isomerase, mitochondrial",
                "CypD",
                "Cyp-D",
                "cyclophilin 3",
                "cyclophilin D",
                "CyP-M",
                "peptidyl-prolyl cis-trans isomerase F, mitochondrial",
                "PPIase F"
            ]
        }
    ],
    "uuid": "0fc35b87-87fb-4ba3-b0ea-1ff17b222048"
}