HG012053

Ian Whaling

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

gRNA sequencing for activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes

activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes

differential gene expression quantifications

activation Perturb-seq: differential gene expression quantifications

GRCh38 differential gene expression quantifications

gene by gene expression by CRISPR guide capture in filtered feature barcode matrix

activation Perturb-seq: filtered feature barcode matrix