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IGVFFI5769PLGK

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS4389OUWU(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
cell by CRISPR guide capture in raw feature barcode matrix
Analysis Step Version
/analysis-step-versions/65c095ee-8330-48d0-bc03-d930326fe393/
File Format
pkl
Content Type
raw feature barcode matrix
Content Summary
cell by CRISPR guide capture in raw feature barcode matrix
Aliases
gary-hon:TF_Perturb_Seq_Pilot_Lenti_CM_PrimaryAnalysis_pkl
md5sum
5f41dff8478740c8d71f66669d15343c
File Size
395 MB
Submitted File Name
/project/GCRB/Hon_lab/s215194/Single_Cell/TF_perturb_seq/_pipeline_reporcessing/_combined_cm/aggr_dataframe/aggr_combined_df_full.pkl
Checkfiles Version
Legacy version of checkfiles
Attribution
Gary Hon, UT

Matrix Details

Principal Dimension
cell
Secondary Dimensions
CRISPR guide capture

Samples

4 items
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW279) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW280) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW278) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW277) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Reference Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
gtf
transcriptome reference
GRCh38 GENCODE 32 transcriptome reference
External Lab, Community
55 MB
validated

Files This File Derives From

128 items
fastq
reads
Gary Hon, UT
717 MB
validated
fastq
reads
Gary Hon, UT
100 MB
validated
fastq
reads
Gary Hon, UT
541 MB
validated
fastq
reads
Gary Hon, UT
262 MB
validated
fastq
reads
Gary Hon, UT
79 MB
validated
fastq
reads
Gary Hon, UT
218 MB
validated
fastq
reads
Gary Hon, UT
97 MB
validated
fastq
reads
Gary Hon, UT
869 MB
validated
fastq
reads
Gary Hon, UT
78 MB
validated
fastq
reads
Gary Hon, UT
89 MB
validated

Files Derived From This File

3 items
h5ad
sparse gene count matrix
cell by gene expression in sparse gene count matrix
Gary Hon, UT
13 GB
validated
csv
unfiltered local differential expression
GRCh38 GENCODE 32 unfiltered local differential expression
Gary Hon, UT
6.8 KB
validated
csv
unfiltered global differential expression
GRCh38 GENCODE 32 unfiltered global differential expression
Gary Hon, UT
734 KB
validated