IGVFFI0296HFSP

{
    "@context": "/terms/",
    "@id": "/matrix-files/IGVFFI0296HFSP/",
    "@type": [
        "MatrixFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI0296HFSP",
    "aliases": [
        "charles-gersbach:cd8-tcells-perturb-seq-10x-5prime-mm-crispri-rep1"
    ],
    "analysis_step_version": "/analysis-step-versions/fbd5e8fb-4206-4b43-92d6-922cdfe1338b/",
    "assay_titles": [
        "Perturb-seq"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG012053/",
        "component": "functional characterization"
    },
    "content_md5sum": "56f1371041624147e07cb088e1c920da",
    "content_summary": "gene by gene expression by CRISPR guide capture in filtered feature barcode matrix",
    "content_type": "filtered feature barcode matrix",
    "creation_timestamp": "2024-09-22T17:45:10.874265+00:00",
    "derived_from": [
        "/sequence-files/IGVFFI2739KXTR/",
        "/sequence-files/IGVFFI1822KONB/",
        "/sequence-files/IGVFFI7019QDVE/",
        "/sequence-files/IGVFFI7645VURG/",
        "/sequence-files/IGVFFI1399GORD/",
        "/sequence-files/IGVFFI7384WZTF/",
        "/sequence-files/IGVFFI6523JOQO/",
        "/sequence-files/IGVFFI7716LYLL/",
        "/sequence-files/IGVFFI5465GDKG/",
        "/sequence-files/IGVFFI1875HEKG/",
        "/sequence-files/IGVFFI3480BTJU/",
        "/sequence-files/IGVFFI5492CFOS/",
        "/sequence-files/IGVFFI4222KPGA/",
        "/sequence-files/IGVFFI7250XPAW/",
        "/sequence-files/IGVFFI9522NHQX/",
        "/sequence-files/IGVFFI5512GZXV/"
    ],
    "file_format": "tar",
    "file_set": {
        "@id": "/analysis-sets/IGVFDS3319RFFN/",
        "accession": "IGVFDS3319RFFN",
        "file_set_type": "intermediate analysis",
        "samples": [
            {
                "@id": "/primary-cells/IGVFSM3795JDHP/",
                "accession": "IGVFSM3795JDHP",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM2747RJFY/",
                "accession": "IGVFSM2747RJFY",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            },
            {
                "@id": "/primary-cells/IGVFSM5189UBOI/",
                "accession": "IGVFSM5189UBOI",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
                        "modality": "interference",
                        "summary": "CRISPRi Sa-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            }
        ],
        "summary": "interference Perturb-seq: filtered feature barcode matrix"
    },
    "file_size": 382505366,
    "gene_list_for": [],
    "href": "/matrix-files/IGVFFI0296HFSP/@@download/IGVFFI0296HFSP.tar.gz",
    "input_file_for": [
        "/tabular-files/IGVFFI5198JCCU/"
    ],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/charles-gersbach/",
        "title": "Charles Gersbach, Duke"
    },
    "loci_list_for": [],
    "md5sum": "016c46d0c12fab6e465c32a04c1c577b",
    "principal_dimension": "gene",
    "reference_files": [
        "/reference-files/IGVFFI0653VCGH/"
    ],
    "release_timestamp": "2024-10-18T19:50:08.516814+00:00",
    "s3_uri": "s3://igvf-files/2024/09/22/b9df5b53-ddfc-4aba-aa69-5f572c717d2a/IGVFFI0296HFSP.tar.gz",
    "schema_version": "7",
    "secondary_dimensions": [
        "gene expression",
        "CRISPR guide capture"
    ],
    "status": "released",
    "submitted_by": {
        "@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
        "title": "Alejandro Barrera"
    },
    "submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/processed_data/AIM_2_T_cell_project_1/CRISPRi_D1_10x.tar.gz",
    "summary": "gene by gene expression by CRISPR guide capture in filtered feature barcode matrix",
    "upload_status": "validated",
    "uuid": "b9df5b53-ddfc-4aba-aa69-5f572c717d2a"
}