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Jurkat — IGVFSM6721RTTV

released
Summary
Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Sample Terms
Classification
cell line
Sex
unspecified
Age
unknown
Taxa
Homo sapiens
Sources
stem-cell-technologies
Aliases
charles-gersbach:jurkats-in-vitro-system-crispra-vp64x2-il2ra-rep2
Publications

Donors

1 item
Accession
Aliases
Sex
Taxa
  • charles-gersbach:jurkat-tcells-donor-5
unspecified
Homo sapiens

File Sets

1 item
Accession
Summary
Aliases
Lab
Homo sapiens GRCh38 custom guide RNAs
  • charles-gersbach:jurkat-il2ra-grnas-set
Charles Gersbach, Duke

Modifications

1 item
Summary
Modality
activation

Sorted Fractions of Sample

2 items
Sample Terms
Summary
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
InVitroSystem
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke