{
"@context": "/terms/",
"@id": "/in-vitro-systems/IGVFSM6721RTTV/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6721RTTV",
"age": "unknown",
"aliases": [
"charles-gersbach:jurkats-in-vitro-system-crispra-vp64x2-il2ra-rep2"
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "mismatched status",
"detail": "Released construct library set [IGVFDS8183QSJO](/construct-library-sets/IGVFDS8183QSJO/) has in progress subobject tabular file [IGVFFI3477BKFD](/tabular-files/IGVFFI3477BKFD/).",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_item_status",
"path": "/construct-library-sets/IGVFDS8183QSJO/"
}
],
"WARNING": [
{
"category": "missing files",
"detail": "Construct library set [IGVFDS8183QSJO](/construct-library-sets/IGVFDS8183QSJO/) has no `files`. File sets are expected to have files.",
"level": 40,
"level_name": "WARNING",
"name": "audit_no_files",
"path": "/construct-library-sets/IGVFDS8183QSJO/"
}
]
},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"classifications": [
"cell line"
],
"construct_library_sets": [
{
"@id": "/construct-library-sets/IGVFDS8183QSJO/",
"accession": "IGVFDS8183QSJO",
"file_set_type": "guide library"
}
],
"creation_timestamp": "2023-11-08T19:29:38.787321+00:00",
"demultiplexed_to": [],
"donors": [
"/human-donors/IGVFDO2560LMUL/"
],
"file_sets": [
{
"@id": "/curated-sets/IGVFDS3467ONBF/",
"accession": "IGVFDS3467ONBF",
"aliases": [
"charles-gersbach:jurkat-il2ra-grnas-set"
],
"file_set_type": "guide RNAs",
"lab": {
"title": "Charles Gersbach, Duke"
},
"status": "in progress",
"summary": "Homo sapiens GRCh38 custom guide RNAs"
}
],
"institutional_certificates": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"modifications": [
{
"@id": "/crispr-modifications/4c532ce1-11aa-41be-9757-13923c19248b/",
"cas": "dCas9",
"cas_species": "Staphylococcus aureus (Sa)",
"fused_domain": "2xVP64",
"modality": "activation",
"status": "released",
"summary": "CRISPRa Sa-dCas9-2xVP64"
}
],
"moi": 0.4,
"multiplexed_in": [],
"notes": "The \"classification\" of this in vitro system was moved into the array property \"classifications.\" This sample's modifications originally included Modification /modifications/8c0f2c0a-c2c0-4d14-98fc-e1551c72667c/ which was deleted and replaced with a Crispr Modification igvf:replacement_of_8c0f2c0a-c2c0-4d14-98fc-e1551c72667c.",
"nucleic_acid_delivery": "lentiviral transduction",
"origin_of": [],
"parts": [],
"pooled_in": [],
"publications": [
{
"@id": "/publications/6cc80a53-4ca1-4bd4-9942-f0f335b7ec20/",
"publication_identifiers": [
"PMID:37945901",
"doi:10.1038/s41588-023-01554-0",
"PMCID:PMC10703699"
]
}
],
"release_timestamp": "2024-11-18T21:08:59.200509+00:00",
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002796/",
"term_name": "Jurkat"
}
],
"schema_version": "23",
"sex": "unspecified",
"sorted_fractions": [
"/in-vitro-systems/IGVFSM6964ODEE/",
"/in-vitro-systems/IGVFSM2429HKWG/"
],
"sources": [
{
"@id": "/sources/stem-cell-technologies/",
"title": "STEMCELL Technologies"
}
],
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"summary": "Jurkat cell line, Homo sapiens modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens",
"uuid": "bdf0a241-12bf-4aa4-bd15-10e7e477e63b",
"virtual": false
}