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HCT116 — IGVFSM5799ZEJH
HCT116 — IGVFSM5799ZEJH
released
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Summary
HCT116 cell line, male, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library
Sample Terms
HCT116
Classification
cell line
Sex
male
Originated From Sample
IGVFSM5760JFFX
Age
unknown
Construct Library Sets
IGVFDS9869EMNE
guide (sgRNA) library targeting accessible genome regions in 29 genomic loci
Taxa
Homo sapiens
Sources
atcc
Aliases
jesse-engreitz:in-vitro-system_CRUDO_TAPseq-Auxin0hrs-Rep1
Donors
1 item
Accession
Aliases
Sex
Taxa
IGVFDO0384OZRW
igvf:donor_of_HCT116
male
Homo sapiens
File Sets
3 items
Accession
Summary
Aliases
Lab
IGVFDS0282GEHF
gRNA sequencing for mixed CRISPR TAP-seq integrating a guide (sgRNA) library targeting accessible genome regions in 29 genomic loci
jesse-engreitz:auxiliary_CRUDO_TAPseq-Auxin0hrs-Rep1
Jesse Engreitz, Stanford
IGVFDS3171OFHP
mixed CRISPR TAP-seq integrating a guide (sgRNA) library targeting accessible genome regions in 29 genomic loci
jesse-engreitz:measurement_CRUDO_TAPseq-Auxin0hrs-Rep1
Jesse Engreitz, Stanford
IGVFDS5308EMCG
degradation, interference TAP-seq: differential element quantifications, guide quantifications
jesse-engreitz:analysis_set_CRUDO_TAPseq
Jesse Engreitz, Stanford
Modifications
2 items
Summary
Modality
Activation
CRISPRi Sp-dCas9-KOX1-KRAB
interference
inactive AID system targeting RAD21
degradation
Documents
2 items
Page
Description
Type
Download
Preview
Generation of inducible CRISPRi line
plasmid map
addgene#85449_TRE-KRAB-dCas9-IRES-BFP.pdf
Download addgene#85449_TRE-KRAB-dCas9-IRES-BFP.pdf
Download addgene#85449_TRE-KRAB-dCas9-IRES-BFP.pdf
Generation of inducible CRISPRi line
plasmid map
takara#631363_pLVX-EF1alpha-Tet3G.pdf
Download takara#631363_pLVX-EF1alpha-Tet3G.pdf
Download takara#631363_pLVX-EF1alpha-Tet3G.pdf
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford