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CRISPRi Sp-dCas9-KOX1-KRAB

released
Summary
CRISPRi Sp-dCas9-KOX1-KRAB
Modality
interference
Cas
dCas9
Cas Species
Streptococcus pyogenes (Sp)
Fused Domain
KOX1-KRAB
Sources
Jesse Engreitz, Stanford addgene:85449
Aliases
igvf:replacement_of_2ba01755-df67-453b-b047-f7cdba236665
jesse-engreitz:modification-teloHAEC-dCas9-KRAB
Submitter Comment
This Crispr Modification is an identical replacement of the previously submitted Modification 2ba01755-df67-453b-b047-f7cdba236665. Doxycyline treatment was done only for the purpose of activating the domain

Biosamples Modified by this Modification

86 items
Sample Terms
Summary
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin A for 0-25% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
K562 cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin for 0-25% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin B for 26-50% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Documents

1 item
Page
Description
Type
Download
Preview
Addgene Plasmid Map TRE-KRAB-dCas9-IRES-BFP
plasmid map

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford