CRISPRi Sp-dCas9-KOX1-KRAB

released
{
    "@context": "/terms/",
    "@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
    "@type": [
        "CrisprModification",
        "Modification",
        "Item"
    ],
    "activated": true,
    "activating_agent_term_id": "CHEBI:50845",
    "activating_agent_term_name": "doxycycline",
    "aliases": [
        "igvf:replacement_of_2ba01755-df67-453b-b047-f7cdba236665",
        "jesse-engreitz:modification-teloHAEC-dCas9-KRAB"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization"
    },
    "biosamples_modified": [
        "/in-vitro-systems/IGVFSM5826EWDE/",
        "/in-vitro-systems/IGVFSM6912FONC/",
        "/in-vitro-systems/IGVFSM9056OSKC/",
        "/in-vitro-systems/IGVFSM7319EZLZ/",
        "/in-vitro-systems/IGVFSM5229SOKC/",
        "/in-vitro-systems/IGVFSM7436UMQD/",
        "/in-vitro-systems/IGVFSM9579BBFC/",
        "/in-vitro-systems/IGVFSM1034FQDF/",
        "/in-vitro-systems/IGVFSM5424HTHI/",
        "/in-vitro-systems/IGVFSM2326VRBK/",
        "/in-vitro-systems/IGVFSM8115WXZU/",
        "/in-vitro-systems/IGVFSM6792NCMN/",
        "/in-vitro-systems/IGVFSM6408QEFG/",
        "/in-vitro-systems/IGVFSM0599MHTG/",
        "/in-vitro-systems/IGVFSM9488LHID/",
        "/in-vitro-systems/IGVFSM8817SMTB/",
        "/in-vitro-systems/IGVFSM1237FNBR/",
        "/in-vitro-systems/IGVFSM2880OLBN/",
        "/in-vitro-systems/IGVFSM6404DFQW/",
        "/in-vitro-systems/IGVFSM6082DZRY/",
        "/in-vitro-systems/IGVFSM1476YHNC/",
        "/in-vitro-systems/IGVFSM6512KVQX/",
        "/in-vitro-systems/IGVFSM7987ARAX/",
        "/in-vitro-systems/IGVFSM6823DARA/",
        "/in-vitro-systems/IGVFSM9750UXVD/",
        "/in-vitro-systems/IGVFSM6969BXDT/",
        "/in-vitro-systems/IGVFSM3814HZQI/",
        "/in-vitro-systems/IGVFSM3700IHHL/",
        "/in-vitro-systems/IGVFSM1061NWCP/",
        "/in-vitro-systems/IGVFSM0862BZMH/",
        "/in-vitro-systems/IGVFSM7408HYIB/",
        "/in-vitro-systems/IGVFSM9054OJLH/",
        "/in-vitro-systems/IGVFSM0914ERRM/",
        "/in-vitro-systems/IGVFSM2486RPXY/",
        "/in-vitro-systems/IGVFSM2756NFXE/",
        "/in-vitro-systems/IGVFSM8121JQAK/",
        "/in-vitro-systems/IGVFSM7992INYA/",
        "/in-vitro-systems/IGVFSM4987QNQG/",
        "/in-vitro-systems/IGVFSM4347MQFL/",
        "/in-vitro-systems/IGVFSM7589OEYV/",
        "/in-vitro-systems/IGVFSM3948PYPE/",
        "/in-vitro-systems/IGVFSM0634QOST/",
        "/in-vitro-systems/IGVFSM2673TSAF/",
        "/in-vitro-systems/IGVFSM2155YTIW/",
        "/in-vitro-systems/IGVFSM9485EHBX/",
        "/in-vitro-systems/IGVFSM0897QVAK/",
        "/in-vitro-systems/IGVFSM3472QIPU/",
        "/in-vitro-systems/IGVFSM0672KXTP/",
        "/in-vitro-systems/IGVFSM4227JQMA/",
        "/in-vitro-systems/IGVFSM7134FIHW/",
        "/in-vitro-systems/IGVFSM9689NFEU/",
        "/in-vitro-systems/IGVFSM3508ZAFL/",
        "/in-vitro-systems/IGVFSM0249YRUJ/",
        "/in-vitro-systems/IGVFSM0180ODAW/",
        "/in-vitro-systems/IGVFSM7471WJWF/",
        "/in-vitro-systems/IGVFSM3812UYWH/",
        "/in-vitro-systems/IGVFSM1575CPXR/",
        "/in-vitro-systems/IGVFSM3104QEEP/",
        "/in-vitro-systems/IGVFSM9362CZWG/",
        "/in-vitro-systems/IGVFSM4878HUHM/",
        "/in-vitro-systems/IGVFSM6467VTYE/",
        "/in-vitro-systems/IGVFSM6909JYCD/",
        "/in-vitro-systems/IGVFSM0912IPPR/",
        "/in-vitro-systems/IGVFSM4464SPLF/",
        "/in-vitro-systems/IGVFSM4675JUYE/",
        "/in-vitro-systems/IGVFSM3569XFKY/",
        "/in-vitro-systems/IGVFSM1085KVBF/",
        "/in-vitro-systems/IGVFSM0325PTVL/",
        "/in-vitro-systems/IGVFSM5205HIZS/",
        "/in-vitro-systems/IGVFSM8559QOCC/",
        "/in-vitro-systems/IGVFSM3988VBUB/",
        "/in-vitro-systems/IGVFSM4639DMNG/",
        "/in-vitro-systems/IGVFSM6643KIQQ/",
        "/in-vitro-systems/IGVFSM3079JHCQ/",
        "/in-vitro-systems/IGVFSM5877PFFV/",
        "/in-vitro-systems/IGVFSM4345IGSA/",
        "/in-vitro-systems/IGVFSM4978VPUD/",
        "/in-vitro-systems/IGVFSM7665HTFX/",
        "/in-vitro-systems/IGVFSM6272EHGA/",
        "/in-vitro-systems/IGVFSM6249JKCM/",
        "/in-vitro-systems/IGVFSM9019CRXH/",
        "/in-vitro-systems/IGVFSM9151FQCE/",
        "/in-vitro-systems/IGVFSM0962TVTG/",
        "/in-vitro-systems/IGVFSM6891FDIO/",
        "/in-vitro-systems/IGVFSM0387IPUP/",
        "/in-vitro-systems/IGVFSM7869DXPV/"
    ],
    "cas": "dCas9",
    "cas_species": "Streptococcus pyogenes (Sp)",
    "creation_timestamp": "2024-06-27T20:48:08.366265+00:00",
    "documents": [
        "/documents/d8ce793f-933b-45ea-9611-1c3f80cb7e11/"
    ],
    "fused_domain": "KOX1-KRAB",
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "modality": "interference",
    "notes": "Fused_domain enum KRAB has been removed.",
    "product_id": "addgene:85449",
    "release_timestamp": "2024-02-07T19:03:01.113073+00:00",
    "schema_version": "4",
    "sources": [
        "/labs/jesse-engreitz/"
    ],
    "status": "released",
    "submitted_by": {
        "@id": "/users/43f2f757-5cbf-490a-9787-a1ee85a4cdcd/",
        "title": "Jennifer Jou"
    },
    "submitter_comment": "This Crispr Modification is an identical replacement of the previously submitted Modification 2ba01755-df67-453b-b047-f7cdba236665. Doxycyline treatment was done only for the purpose of activating the domain",
    "summary": "CRISPRi Sp-dCas9-KOX1-KRAB",
    "uuid": "b4bc725a-8eeb-4180-9789-bece3516c0c1"
}