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INDEXED
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ConstructLibrarySet
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IGVFDS9830PJVQ
IGVFDS9830PJVQ
released
Json view
File Set Type
guide library
Control Type
non-targeting
Summary
non-targeting guide (sgRNA) library
Aliases
charles-gersbach:cd8-tcell-ko-nt-grna-construct-lib-set
Publications
Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens
Attribution
Charles Gersbach, Duke
(
HG012053
—
Charles Gersbach
)
Guide Library Details
Library Construct Scope
control
Selection Criteria
transcription start sites
Guide Type
sgRNA
Tiling Modality
sparse peaks
Average Guide Coverage
1
Integrated Content Files
1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
IGVFFI7982RBWP
tsv
guide RNA sequences
GRCh38 guide RNA sequences
Charles Gersbach, Duke
200 B
validated
File Sets Controlled by This Construct Library Set
2 items
Accession
Type
Summary
Aliases
Lab
IGVFDS2398ZXBQ
ConstructLibrarySet
guide (sgRNA) library targeting transcription start sites in GATA3
charles-gersbach:cd8-tcell-ko-gata3-grna-construct-lib-set
Charles Gersbach, Duke
IGVFDS7189YPQQ
ConstructLibrarySet
guide (sgRNA) library targeting transcription start sites in ZNF217
charles-gersbach:cd8-tcell-ko-znf217-grna-construct-lib-set
Charles Gersbach, Duke
Applied to Samples
3 items
Accession
Type
Sample Terms
Summary
IGVFSM0383IKGE
PrimaryCell
CD8-positive, alpha-beta memory T cell
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
IGVFSM1498WFCO
PrimaryCell
CD8-positive, alpha-beta memory T cell
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
IGVFSM6537MPGY
PrimaryCell
CD8-positive, alpha-beta memory T cell
Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
Documents
1 item
Page
Description
Type
Download
Standardized File Format
Preview
SpCas9-gRNA-hUbC-mCherry-2A-PuroR Map
plasmid map
pSM73-SpCas9-gRNA-hUbC-mCherry-2A-PuroR Map.pdf
Download pSM73-SpCas9-gRNA-hUbC-mCherry-2A-PuroR Map.pdf
Download pSM73-SpCas9-gRNA-hUbC-mCherry-2A-PuroR Map.pdf