{
"@context": "/terms/",
"@id": "/construct-library-sets/IGVFDS9830PJVQ/",
"@type": [
"ConstructLibrarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS9830PJVQ",
"aliases": [
"charles-gersbach:cd8-tcell-ko-nt-grna-construct-lib-set"
],
"applied_to_samples": [
{
"@id": "/primary-cells/IGVFSM1498WFCO/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1498WFCO",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-cas9-ko-nt-rep2"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO7589UJKG/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM6537MPGY/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6537MPGY",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-cas9-ko-nt-rep1"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO8565PQXZ/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
},
{
"@id": "/primary-cells/IGVFSM0383IKGE/",
"@type": [
"PrimaryCell",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0383IKGE",
"aliases": [
"charles-gersbach:cd8-tcells-primary-cell-cas9-ko-nt-rep3"
],
"classifications": [
"primary cell"
],
"donors": [
{
"@id": "/human-donors/IGVFDO4048LCOU/",
"status": "released",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/9fef21f2-9727-4a97-a5a7-c590a637c101/",
"modality": "knockout",
"status": "released",
"summary": "CRISPRko Sp-Cas9"
}
],
"nucleic_acid_delivery": "lentiviral transduction",
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)"
}
],
"audit": {
"INTERNAL_ACTION": [
{
"category": "missing publication",
"detail": "Construct library set [IGVFDS9830PJVQ](/construct-library-sets/IGVFDS9830PJVQ/) has no `publications`. Released and archived file sets are expected to be associated with a publication.",
"level": 30,
"level_name": "INTERNAL_ACTION",
"name": "audit_file_set_missing_publication",
"path": "/construct-library-sets/IGVFDS9830PJVQ/"
}
],
"NOT_COMPLIANT": [
{
"category": "missing control for",
"detail": "Construct library set [IGVFDS9830PJVQ](/construct-library-sets/IGVFDS9830PJVQ/) has no `control_for`. The `control_file_sets` should be patched on the file sets it serves as a control for and this property, `control_for`, will be reverse calculated. File sets that specify a control type are expected to be a control for other file sets.",
"level": 50,
"level_name": "NOT_COMPLIANT",
"name": "audit_control_for_control_type",
"path": "/construct-library-sets/IGVFDS9830PJVQ/"
}
]
},
"average_guide_coverage": 1,
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"control_for": [],
"control_type": "non-targeting",
"creation_timestamp": "2023-11-08T18:25:49.331463+00:00",
"documents": [
"/documents/2850de29-469d-445e-b303-9753b6f878ea/"
],
"file_set_type": "guide library",
"files": [],
"guide_type": "sgRNA",
"input_for": [],
"integrated_content_files": [
{
"@id": "/tabular-files/IGVFFI4223LNAY/",
"accession": "IGVFFI4223LNAY",
"aliases": [
"charles-gersbach:cd8-tcell-ko-nt-grnas-metadata"
],
"content_type": "guide RNA sequences",
"file_format": "tsv",
"status": "released",
"upload_status": "validated"
}
],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"release_timestamp": "2024-10-25T04:18:35.933681+00:00",
"schema_version": "10",
"scope": "control",
"selection_criteria": [
"transcription start sites"
],
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"summary": "non-targeting guide (sgRNA) library",
"tiling_modality": "sparse peaks",
"uuid": "832631f1-2598-4a03-b570-9642433fdd81"
}