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IGVFDS8953DFJK

released
File Set Type
guide library
Summary
guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3
Aliases
jesse-engreitz:construct_library_NOS3_CRISPRi_FF

Guide Library Details

Library Construct Scope
genes
Selection Criteria
sequence variants, transcription start sites
Small Scale Gene List
Guide Type
sgRNA
Tiling Modality
sparse peaks

Sequencing Results (Illumina)

2 items
fastq
reads
R1
1
116 MB
Jesse Engreitz, Stanford
validated
fastq
reads
I1
1
38 MB
Jesse Engreitz, Stanford
validated

Integrated Content Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
guide RNA sequences
GRCh38 guide RNA sequences
Jesse Engreitz, Stanford
2.0 KB
validated

Applied to Samples

38 items
Sample Terms
Summary
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin A for 0-25% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin B for 26-50% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Documents

2 items
Page
Preview
sgOpti-blast gRNA plasmid map
plasmid map
sgOpti-blast gRNA plasmid sequence
plasmid map

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford