{
"@context": "/terms/",
"@id": "/construct-library-sets/IGVFDS8953DFJK/",
"@type": [
"ConstructLibrarySet",
"FileSet",
"Item"
],
"accession": "IGVFDS8953DFJK",
"aliases": [
"jesse-engreitz:construct_library_NOS3_CRISPRi_FF"
],
"applied_to_samples": [
{
"@id": "/in-vitro-systems/IGVFSM6792NCMN/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6792NCMN",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep1_binA_PCR1_S113"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin A for 0-25% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM4227JQMA/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4227JQMA",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep2_binD_PCR1_S124"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM8817SMTB/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM8817SMTB",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep2_binA_PCR2_S201"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin A for 0-25% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM9488LHID/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM9488LHID",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep1_binB_PCR1_S114"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin B for 26-50% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM4639DMNG/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4639DMNG",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep1_binC_PCR1_S115"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM0325PTVL/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0325PTVL",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep1_binD_PCR1_S116"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM4675JUYE/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4675JUYE",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep1_binA_PCR2_S193"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin A for 0-25% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM3988VBUB/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3988VBUB",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep1_binB_PCR2_S194"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin B for 26-50% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM4347MQFL/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4347MQFL",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep2_binD_PCR1_S140"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM3948PYPE/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3948PYPE",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep1_binC_PCR2_S195"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM6512KVQX/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6512KVQX",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep2_binA_PCR1_S121"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin A for 0-25% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM4464SPLF/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4464SPLF",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep2_binB_PCR1_S122"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin B for 26-50% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM7665HTFX/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7665HTFX",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep2_binC_PCR1_S123"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM3569XFKY/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3569XFKY",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep2_binD_PCR2_S204"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM6909JYCD/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6909JYCD",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep2_binC_PCR2_S219"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM1237FNBR/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1237FNBR",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep1_binD_PCR2_S196"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM7992INYA/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7992INYA",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep1_binB_PCR1_S130"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin B for 26-50% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM0912IPPR/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0912IPPR",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep1_binB_PCR2_S210"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin B for 26-50% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM7471WJWF/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7471WJWF",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep1_binC_PCR2_S211"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM4345IGSA/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4345IGSA",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep2_binB_PCR2_S202"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin B for 26-50% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM1476YHNC/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1476YHNC",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep1_binA_PCR1_S129"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin A for 0-25% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM5205HIZS/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM5205HIZS",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep1_binA_PCR2_S209"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin A for 0-25% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM4987QNQG/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4987QNQG",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep2_binC_PCR2_S203"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM3812UYWH/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3812UYWH",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep1_binC_PCR1_S131"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM0634QOST/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0634QOST",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep1_binD_PCR1_S132"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM4978VPUD/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM4978VPUD",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep1_binD_PCR2_S212"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM5877PFFV/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM5877PFFV",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep2_binB_PCR1_S138"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin B for 26-50% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM0180ODAW/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0180ODAW",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep2_binA_PCR2_S217"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin A for 0-25% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM3079JHCQ/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM3079JHCQ",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep2_binA_PCR1_S137"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin A for 0-25% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM0249YRUJ/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0249YRUJ",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep2_binC_PCR1_S139"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM8559QOCC/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM8559QOCC",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep2_binB_PCR2_S218"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin B for 26-50% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM7134FIHW/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7134FIHW",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep2_binD_PCR2_S220"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM1085KVBF/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1085KVBF",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM7589OEYV/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM7589OEYV",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep2"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM0897QVAK/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0897QVAK",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R1_FFrep1"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM0672KXTP/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM0672KXTP",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM1034FQDF/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM1034FQDF",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep1"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
},
{
"@id": "/in-vitro-systems/IGVFSM6082DZRY/",
"@type": [
"InVitroSystem",
"Biosample",
"Sample",
"Item"
],
"accession": "IGVFSM6082DZRY",
"aliases": [
"jesse-engreitz:in-vitro-system_NOS3_CRISPRi_FF_R2_FFrep2"
],
"classifications": [
"cell line"
],
"donors": [
{
"@id": "/human-donors/IGVFDO3459DFTW/",
"taxa": "Homo sapiens"
}
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library"
}
],
"audit": {
"NOT_COMPLIANT": [
{
"category": "missing sequence specification",
"detail": "Construct library set [IGVFDS8953DFJK](/construct-library-sets/IGVFDS8953DFJK/) has sequence file(s): [IGVFFI9158VFIB](/sequence-files/IGVFFI9158VFIB/), [IGVFFI7019XQBX](/sequence-files/IGVFFI7019XQBX/) which do not have any `seqspecs`. Sequence files in a file set are expected to link to a sequence specification file.",
"level": 50,
"level_name": "NOT_COMPLIANT",
"name": "audit_missing_seqspec",
"path": "/construct-library-sets/IGVFDS8953DFJK/"
}
],
"WARNING": [
{
"category": "missing analysis",
"detail": "Construct library set [IGVFDS8953DFJK](/construct-library-sets/IGVFDS8953DFJK/) is a raw data set with files, but is not listed in any `input_file_sets` for any analysis sets. Raw data sets with files are expected to be associated with at least one analysis set.",
"level": 40,
"level_name": "WARNING",
"name": "audit_input_for",
"path": "/construct-library-sets/IGVFDS8953DFJK/"
}
]
},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"control_for": [],
"creation_timestamp": "2024-04-22T20:38:53.029366+00:00",
"documents": [
"/documents/475af277-70a7-4734-97c5-c6fffea795c6/",
"/documents/a1610beb-0637-4d13-b2ef-def0c3afaca2/"
],
"file_set_type": "guide library",
"files": [
{
"@id": "/sequence-files/IGVFFI9158VFIB/",
"accession": "IGVFFI9158VFIB",
"content_type": "reads",
"file_format": "fastq",
"upload_status": "validated"
},
{
"@id": "/sequence-files/IGVFFI7019XQBX/",
"accession": "IGVFFI7019XQBX",
"content_type": "reads",
"file_format": "fastq",
"upload_status": "validated"
}
],
"guide_type": "sgRNA",
"input_for": [],
"integrated_content_files": [
{
"@id": "/tabular-files/IGVFFI5833LNWN/",
"accession": "IGVFFI5833LNWN",
"content_type": "guide RNA sequences",
"file_format": "tsv",
"upload_status": "validated"
}
],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"release_timestamp": "2024-06-22T00:20:31.427620+00:00",
"schema_version": "10",
"scope": "genes",
"selection_criteria": [
"sequence variants",
"transcription start sites"
],
"small_scale_gene_list": [
{
"@id": "/genes/ENSG00000164867/",
"geneid": "ENSG00000164867",
"name": "nitric oxide synthase 3",
"symbol": "NOS3",
"synonyms": [
"cNOS",
"constitutive NOS",
"EC-NOS",
"nitric oxide synthase, endothelial",
"eNOS",
"ECNOS",
"NOS type III",
"NOSIII",
"nitric oxide synthase 3 (endothelial cell)",
"endothelial NOS"
]
}
],
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"submitted_files_timestamp": "2024-04-23T17:06:41.920606+00:00",
"summary": "Guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3",
"tiling_modality": "sparse peaks",
"uuid": "c55aec63-39c5-426d-9599-af5782669fc7"
}