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IGVFDS7581DNXF

released
File Set Type
guide library
Summary
guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
Aliases
jay-shendure:construct_library_CRISPRa_Chardon-McDiarmid
Publications
Attribution
Jay Shendure, UW

Guide Library Details

Library Construct Scope
loci
Selection Criteria
TF binding sites, candidate cis-regulatory elements
Large Scale Loci List
IGVFFI2463QNJD
Guide Type
sgRNA

Integrated Content Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
guide RNA sequences
GRCh38 guide RNA sequences
Jay Shendure, UW
12 KB
validated

Applied to Samples

11 items
Sample Terms
Summary
InVitroSystem
Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane1) modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library
InVitroSystem
Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane1) modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane1) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane3) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library
InVitroSystem
Homo sapiens (female) K562 cell line modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane4) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library
InVitroSystem
Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane2) modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library
InVitroSystem
Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane2) modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library
InVitroSystem
Homo sapiens (female) K562 cell line modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library

Documents

2 items
Page
Preview
Plasmid map for scCRISPRa Piggybac expression vector
plasmid map
Plasmid map for scCRISPRa Piggybac expression vector
plasmid map