{
"@context": "/terms/",
"@id": "/configuration-files/IGVFFI9850DZDZ/",
"@type": [
"ConfigurationFile",
"File",
"Item"
],
"accession": "IGVFFI9850DZDZ",
"aliases": [
"jesse-engreitz:seqspecfile_CRUDO_KITLG-Auxin0hrs-FF4-BinA-PCR2_S43"
],
"assay_titles": [
"CRISPR FlowFISH screen"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "e6791329865dd3c8c10f3bed1c82e44a",
"content_type": "seqspec",
"creation_timestamp": "2024-06-11T22:08:35.351205+00:00",
"file_format": "yaml",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS6495PTGH/",
"accession": "IGVFDS6495PTGH",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM1476GCQU/",
"accession": "IGVFSM1476GCQU",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
},
{
"@id": "/degron-modifications/2e8d10cc-fc98-44d5-a7d9-e2892e16c98a/",
"modality": "degradation",
"status": "released",
"summary": "inactive AID system targeting RAD21"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002824/",
"status": "released",
"term_name": "HCT116"
}
],
"status": "released",
"summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "released",
"summary": "gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
},
"file_size": 903,
"gene_list_for": [],
"href": "/configuration-files/IGVFFI9850DZDZ/@@download/IGVFFI9850DZDZ.yaml.gz",
"input_file_for": [],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"md5sum": "3607ecb0759279e9bad26c02625132f8",
"release_timestamp": "2024-07-10T20:15:38.661998+00:00",
"s3_uri": "s3://igvf-files/2024/06/11/cd0cab8e-0cc9-47c0-bf02-85b08a4ece58/IGVFFI9850DZDZ.yaml.gz",
"schema_version": "7",
"seqspec_of": [
"/sequence-files/IGVFFI1299YDLA/"
],
"status": "released",
"submitted_by": {
"@id": "/users/100f06c6-83a7-4b4d-9dfd-053a1829773b/",
"title": "Benjamin Doughty"
},
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.Auxin0hrs-FF4-BinA-PCR2_S43.yaml",
"summary": "seqspec of IGVFFI1299YDLA",
"upload_status": "validated",
"uuid": "cd0cab8e-0cc9-47c0-bf02-85b08a4ece58"
}