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IGVFFI9179ZFTQ

released
validated
File Set
IGVFDS4623YYCA(mixed CRISPR FlowFISH screen sorted on the expression of KITLG integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
seqspec of IGVFFI4644CRZB
File Format
yaml
Content Type
seqspec
Aliases
jesse-engreitz:seqspecfile_CRUDO_KITLG-Auxin0hrs-FF2-BinF-PCR3_S114
md5sum
f69f82e9606ce55f5c79d1d592fc109a
Content MD5sum
c55eab61f24f90b8ebb56138817da34f
File Size
903 B
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.Auxin0hrs-FF2-BinF-PCR3_S114.yaml
Checkfiles Version
Legacy version of checkfiles

seqspec File Of

1 item
Accession
File Format
Content Type
Sequencing Run
Read Names
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
1
TCTGGCGA:ATTG
22 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford