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IGVFFI8734RNZH

released
validated
File Set
IGVFDS7994GDON(gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in many genes)
Summary
seqspec of IGVFFI0515QEWR, IGVFFI0944TCGC
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:hPA-Seqspec-perturbseq-10xlane1-CRISPR-L1
md5sum
43df6f875cfe988eedcc3484c77d1ada
Content MD5sum
492ac7505b5fad8b4d2d001a4eb2447b
File Size
1.4 KB
Submitted File Name
/work/rr151/SamAIM2neuro_data/AstrocytehPA-Seqspec-10xlane1-crispr-L1.yaml.gz
Checkfiles Version
v20
Attribution
Charles Gersbach, Duke

seqspec File Of

2 items
Read Names
fastq
reads
4
Read 1
1
1.0 GB
Charles Gersbach, Duke
validated
fastq
reads
4
Read 2
1
1.5 GB
Charles Gersbach, Duke
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
Homo sapiens astrocyte cell treated with 10 ng/mL Neurotrophin-3, 20 ng/mL Brain-Derived Neurotrophic Factor, modified with CRISPRa Sp-dCas9-2xVP64-2A-Puro, transduced (lentivirus) with a guide library