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IGVFFI8536YCHC
IGVFFI8536YCHC
released
validated
Json view
File Set
IGVFDS0238VKNF
(CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
seqspec of IGVFFI6761ZNFE, IGVFFI6787BWWZ
File Format
yaml
Content Type
seqspec
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispra-gex-rep3-rep3-fastq-lane1_seqspec
md5sum
6770da6a1a0eb419434b4b159d6acc1b
Content MD5sum
e0be52c1e976a059854c20064487018d
File Size
982 B
Submitted File Name
/datacommons/gersbachlab/Ruhi/IGVFseqspec/CRISPRaD3_S6nS12_L001.rna.yaml.gz
seqspec File Of
2 items
Accession
File Format
Content Type
Sequencing Run
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
IGVFFI6761ZNFE
fastq
reads
3
Illumina NovaSeq 6000
1
4.6 GB
Charles Gersbach, Duke
validated
IGVFFI6787BWWZ
fastq
reads
3
Illumina NovaSeq 6000
1
11 GB
Charles Gersbach, Duke
validated
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM2105CLXS
PrimaryCell
CD8-positive, alpha-beta memory T cell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
Attribution
Award
HG012053
Principal Investigator(s)
Charles Gersbach
,
Gregory Crawford
,
Tim Reddy
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke