IGVFFI8205LBRH

released
validated
1{
2    "@context": "/terms/",
3    "@id": "/configuration-files/IGVFFI8205LBRH/",
4    "@type": [
5        "ConfigurationFile",
6        "File",
7        "Item"
8    ],
9    "accession": "IGVFFI8205LBRH",
10    "aliases": [
11        "jay-shendure:CRISPRa_singlecell_K562_VP64_10Xlane1_seqlane1_exprs_seqspec"
12    ],
13    "assay_titles": [
14        "scCRISPR screen"
15    ],
16    "audit": {},
17    "award": {
18        "@id": "/awards/HG011966/",
19        "component": "functional characterization"
20    },
21    "checkfiles_version": "v20",
22    "content_md5sum": "2b9f15eb912949a24e9c67be260f47a2",
23    "content_type": "seqspec",
24    "creation_timestamp": "2025-04-02T18:25:54.257716+00:00",
25    "derived_manually": false,
26    "file_format": "yaml",
27    "file_set": {
28        "@id": "/measurement-sets/IGVFDS4863BMGZ/",
29        "@type": [
30            "MeasurementSet",
31            "FileSet",
32            "Item"
33        ],
34        "accession": "IGVFDS4863BMGZ",
35        "assay_term": {
36            "@id": "/assay-terms/OBI_0003660/",
37            "term_name": "in vitro CRISPR screen using single-cell RNA-seq"
38        },
39        "data_use_limitation_summaries": [
40            "no certificate"
41        ],
42        "file_set_type": "experimental data",
43        "samples": [
44            {
45                "@id": "/in-vitro-systems/IGVFSM4521QSSO/",
46                "@type": [
47                    "InVitroSystem",
48                    "Biosample",
49                    "Sample",
50                    "Item"
51                ],
52                "accession": "IGVFSM4521QSSO",
53                "classifications": [
54                    "cell line"
55                ],
56                "modifications": [
57                    {
58                        "@id": "/crispr-modifications/ee99bf66-2a5a-4ec6-8380-9434b259afd1/",
59                        "modality": "activation",
60                        "status": "released",
61                        "summary": "CRISPRa Sp-dCas9-VP64"
62                    }
63                ],
64                "sample_terms": [
65                    {
66                        "@id": "/sample-terms/EFO_0002067/",
67                        "status": "released",
68                        "term_name": "K562"
69                    }
70                ],
71                "status": "released",
72                "summary": "Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane1) modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library",
73                "taxa": "Homo sapiens"
74            }
75        ],
76        "status": "released",
77        "summary": "scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
78    },
79    "file_size": 1264,
80    "gene_list_for": [],
81    "href": "/configuration-files/IGVFFI8205LBRH/@@download/IGVFFI8205LBRH.yaml.gz",
82    "input_file_for": [],
83    "integrated_in": [],
84    "lab": {
85        "@id": "/labs/jay-shendure/",
86        "title": "Jay Shendure, UW"
87    },
88    "loci_list_for": [],
89    "md5sum": "11e15f990a56e9019a9223c74f8c8295",
90    "quality_metrics": [],
91    "release_timestamp": "2025-05-20T16:22:35.791259+00:00",
92    "s3_uri": "s3://igvf-files/2025/04/02/218d0936-ff52-4ba6-b543-043842fcdf1a/IGVFFI8205LBRH.yaml.gz",
93    "schema_version": "8",
94    "seqspec_of": [
95        "/sequence-files/IGVFFI0072NENY/",
96        "/sequence-files/IGVFFI3105RHFR/",
97        "/sequence-files/IGVFFI5106LXXU/"
98    ],
99    "status": "released",
100    "submitted_by": {
101        "@id": "/users/73f58e9f-8f5d-4fee-a7cd-dd63c0167e4b/",
102        "title": "Beth Martin"
103    },
104    "submitted_file_name": "/net/shendure/vol1/home/martin91/IGVF/forupload/seqspecs/CRISPRa/CRISPRa_IGVFDS4863BMGZ_1_exprs_seqspec_v3.yaml.gz",
105    "summary": "seqspec of IGVFFI0072NENY, IGVFFI3105RHFR, IGVFFI5106LXXU",
106    "upload_status": "validated",
107    "uuid": "218d0936-ff52-4ba6-b543-043842fcdf1a",
108    "validate_onlist_files": true
109}