IGVFFI8004NCEQ

{
    "@context": "/terms/",
    "@id": "/configuration-files/IGVFFI8004NCEQ/",
    "@type": [
        "ConfigurationFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI8004NCEQ",
    "aliases": [
        "charles-gersbach:cd8-tcells-perturb-seq-crispra-grna-rep2-rep2-fastq-lane1_seqspec"
    ],
    "assay_titles": [
        "Perturb-seq"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG012053/",
        "component": "functional characterization"
    },
    "content_md5sum": "b9b3bc8c8e27dd4aeb871031b99dca97",
    "content_type": "seqspec",
    "creation_timestamp": "2024-09-20T16:49:50.926062+00:00",
    "file_format": "yaml",
    "file_set": {
        "@id": "/auxiliary-sets/IGVFDS0993OMZM/",
        "accession": "IGVFDS0993OMZM",
        "file_set_type": "gRNA sequencing",
        "samples": [
            {
                "@id": "/primary-cells/IGVFSM6223YCQV/",
                "accession": "IGVFSM6223YCQV",
                "classifications": [
                    "primary cell"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/2c49eb31-f10b-47b7-b260-4f720014ad79/",
                        "modality": "activation",
                        "summary": "CRISPRa Sa-dCas9-VP64"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/CL_0000909/",
                        "term_name": "CD8-positive, alpha-beta memory T cell"
                    }
                ],
                "summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)",
                "taxa": "Homo sapiens"
            }
        ],
        "summary": "gRNA sequencing for activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes"
    },
    "file_size": 1079,
    "gene_list_for": [],
    "href": "/configuration-files/IGVFFI8004NCEQ/@@download/IGVFFI8004NCEQ.yaml.gz",
    "input_file_for": [],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/charles-gersbach/",
        "title": "Charles Gersbach, Duke"
    },
    "loci_list_for": [],
    "md5sum": "f1f64843e0479c6f92db2ce620ae9a91",
    "release_timestamp": "2024-10-18T19:49:57.362066+00:00",
    "s3_uri": "s3://igvf-files/2024/09/20/3d85a074-3a87-4fd0-94f3-ba61685ec3a5/IGVFFI8004NCEQ.yaml.gz",
    "schema_version": "7",
    "seqspec_of": [
        "/sequence-files/IGVFFI5776QDOU/",
        "/sequence-files/IGVFFI2156GTTL/"
    ],
    "status": "released",
    "submitted_by": {
        "@id": "/users/3782ce69-0e9f-4b46-91f9-4578139785c7/",
        "title": "Ruhi Rai"
    },
    "submitted_file_name": "/datacommons/gersbachlab/Ruhi/IGVFseqspec/CRISPRaD2_S5nS11_L001.crispr.yaml.gz",
    "summary": "seqspec of IGVFFI5776QDOU, IGVFFI2156GTTL",
    "upload_status": "validated",
    "uuid": "3d85a074-3a87-4fd0-94f3-ba61685ec3a5"
}