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IGVFFI7209PXFH

released
validation exempted
File Set
IGVFDS1261RWQY(mixed CRISPR FlowFISH screen sorted on the expression of ITPRID2 integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
seqspec of IGVFFI4418OJED
File Format
yaml
Content Type
seqspec
Aliases
jesse-engreitz:seqspecfile_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR4-Rep1_S89
md5sum
7f1c559996c1184122ab76b0ec049e98
Content MD5sum
b62885b7cdaea3713bf938d845aac95e
File Size
913 B
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/240410_IGVF_upload/seqspec/guideseq.out.SSFA2-Auxin6hrs-FF1-BinE-PCR4-Rep1_S89.yaml
Checkfiles Version
No version due to exemption
Attribution
Jesse Engreitz, Stanford

seqspec File Of

1 item
Accession
File Format
Content Type
Sequencing Run
Read Names
Sequencing Platform
Flowcell ID
Lane
Index
File Size
Lab
Upload Status
fastq
reads
1
TTACGCAC:GCCA
20 MB
Jesse Engreitz, Stanford
validated

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) HCT116 cell line (sorting details: FACS bin E for 80-90% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library